LOCUS Exported 9013 bp ds-DNA circular SYN 12-JUN-2014 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled1 - Sequence SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9013) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 19, 2018 from SnapGene Viewer 4.1.8 http://www.snapgene.com COMMENT New NA Sequence FEATURES Location/Qualifiers source 1..9013 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..172 /label=TPL2/3 /note="TPL2/3" misc_feature 154..156 /label=SD /note="SD" promoter 181..331 /note="MLP" misc_feature 339..341 /label=SA /note="SA" CDS 352..408 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /note="residues deleted (2012-01-10)" /translation="ATMEFQTQVLMSLLLCMSG" CDS 409..416 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /translation="AA" CDS 417..1770 /codon_start=1 /label=PF3D7_0721700 /note="residues deleted (2014-06-12)" /translation="RPALQRKIRQRGQAPGQRVQEAEPRRRQALQPERKHQLRAEEQLR AVRGRQRRAEAAELQRERAQTGAEEKHGQVPLPDQRPGSAVRRVRAERPGQRKGAERDT EEREPLLHNRRPGVQQPEPDQRGR*PHLRPADPGRAGPELRSEQRPGHLRDPEQYRRAP GLILLPRNETGPLPAALPRGPLRQQKRHQEQGYQLPVPSARVPDRRDADHQIRPQVQLQ RRRPGRERTVPRDPDQHPGNRQPAARAERGQLQERAERAAGHPAEYCQGHPLPEGGGPG RAGQRHHQPRGTDPGQQELQQLQREHQEFRVQLRQEQPGHLQHGPVLSGPEPEPVRADP ERRA*QQPADKHCRDQRADQQRRLPDPLHPEAGPGKQPVPDRGRPGQRQEHLRAQGHPE HRDSGVLRRALHLGHHGQPSGRLLLHLRQPGVRYRQVLQLQCEQPGLLRAEP" CDS 1779..2327 /codon_start=1 /label=AscI CD4d3+4 /note="AscI CD4d3+4" /note="residues deleted (2006-03-14)" /translation="VDLHHGL*E*GGVSGVLLPTQPWRGKPAGRVEMEGREGSFFPVLD HLLPKEPKGVCAEVY*QPQVPAVRNAPTHPSDTPGLPSVCWFWQPDPDSGQRDTVSGSE PGGDESDSARQQHFDL*GDGTHLTQDETDLEAGESGGQGLQAGESDSSAGP*SRGVAMS TE*R*RGQDGLQDPGFIQRV" misc_feature complement(1999..2018) misc_feature 1999..2018 CDS 2328..3330 /codon_start=1 /label=L1 - COMP - L2 - Blac /note="L1 - COMP - L2 - Blac" /note="residues deleted (2010-08-05)" /translation="EFRRRLRRRYRWRRPSPTDASRTPGD*CGAARRERALATAGQGDH LPEEYGDGM*RLRSRQKPASTSTSGPTPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP* EFSPRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWH DSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKR RA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**I WSR*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*AL" misc_feature 3331..3443 /label=Flag-His /note="Flag-His" 3'UTR 3457..3551 /label=polyA /note="polyA" misc_feature complement(3619..3638) rep_origin 3848..5714 /label=OriP /note="OriP" CDS 5946..6806 /codon_start=1 /label=AmpR /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 6951..7624 /label=pUC origin /note="pUC origin" promoter 8254..8842 /note="CMV promoter" TATA_signal 8807..8812 /note="TATA box" misc_feature 8846..8865 /label=TPL1 /note="TPL1" misc_feature 8883..9013 /label=TPL2/3 /note="TPL2/3" ORIGIN 1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg 61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta 121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt 181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca 241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg 301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg 361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccgtc 421 cggccctaca aagaaagata cggcaacgtg gccaagcacc tggacaacga gttcaagaag 481 cggaaccccg acggcggcaa gcccttcaac ctgaaagaaa acatcaacta cgtgcggaag 541 aacaactacg agctgtacgt ggacgccaac ggcgagctga agcagcagaa ctacaacgtg 601 aacgagccca gacaggtgct gaagaaaaac atggacaagt tccgctacct gatcaacgac 661 ctggaagtgc tgttcggcgt gtacgagctg aacgcccggg acaaagaaaa ggtgctgaga 721 gagacactga agaaagagag cctctgcttc acaatcgccg gcctggtgtc caacagcctg 781 aaccagatca acgtggaaga tagccccatc tacggcctgc tgatccaggg cgagcgggac 841 cggaactacg aagtgaacaa cgccctggac atctacgtga tcctgagcaa tatcgccgtg 901 cccctgggct cattcttctt ccccgaaatg aaacgggccc cttaccagct gccctacctc 961 gtgggccact acgacagcag aagcggcatc aagaacaagg ctatcagcta cctgtgccct 1021 ctgcccgagt tcctgatcga cgtgatgcgg accaccaaat acggcctcaa gttcaacttc 1081 aacggcgccg acctggacgt gaaagaactg ttccccgtga ccctgaccaa caccctggaa 1141 accgccagcc tgctgcccgt gcagaaagag gacagctaca agaacgagcg gaacgtgctg 1201 ctgggcatcc tgcagaatat tgccaaggac atcccctacc ggaaggtggt ggaccggggc 1261 gtgcagggca acggcatcat caaccccgag gaacggatcc tggccagcaa gagcttcagc 1321 agctccagag agaacatcaa gaatttcgtg tccaactccg gcaagagcag cctggacacc 1381 tccagcatgg ccctgtactt tctggccctg agccagaacc tgttcgagcg gacccggaac 1441 gccgtgccta gcagcaaccg gctgacaaac attgccgtga ccaacgtgca gaccaacaac 1501 gccggctacc tgacccacta caccctgaag ctgggcctgg aaaacagccg gtccctgacc 1561 gtggaaggcc tggtcaacgg caagaacatc ttcgtgccca agggcatcca gaacatcgag 1621 acagtggtgt cctacgacgt gccctacacc tggggcatca tggtcagccc tcaggaaggc 1681 ttctcctaca tcttcggcaa cctggtgttc gatatcgcca agtccttcag ctacaatgtg 1741 aacaacctgg cctccttcgt gcggagccct ggcgcgccgt cgacctccat cacggcctat 1801 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg 1861 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc 1921 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg 1981 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct 2041 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg 2101 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca 2161 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag 2221 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt 2281 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttccggcggc 2341 ggctccggag gcggtaccgg tggcggcgac ctagccccac agatgcttcg agaactccag 2401 gagactaatg cggcgctgca agacgtgaga gagctcttgc gacagcaggt caaggagatc 2461 accttcctga agaatacggt gatggaatgt gacgcctgcg gtctagacag aaacctgcct 2521 ccactagcac ctctgggccc acacccagaa acgctggtga aagtaaaaga tgctgaagat 2581 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 2641 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 2701 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 2761 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 2821 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 2881 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 2941 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 3001 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 3061 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 3121 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 3181 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 3241 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 3301 gagataggtg cctcactgat taagcattgg ggccctgact acaaagacca tgacggtgat 3361 tataaagatc atgacatcga ttacaaggat gacgatgaca agggccctca ccaccaccat 3421 caccactaat taattaagga tcccccgacc tcgacctctg gctaataaag gaaatttatt 3481 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg 3541 gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat ccccgccccg 3601 gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc 3661 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg 3721 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat 3781 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac 3841 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt 3901 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg 3961 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc 4021 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta 4081 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag 4141 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta 4201 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa 4261 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt 4321 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa 4381 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg 4441 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat 4501 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac 4561 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg 4621 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta 4681 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt 4741 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga 4801 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc 4861 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc 4921 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct 4981 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt 5041 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg 5101 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata 5161 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta 5221 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta 5281 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta 5341 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta 5401 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat 5461 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat 5521 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat 5581 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat 5641 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat 5701 atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga aagggcctcg 5761 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 5821 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 5881 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 5941 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 6001 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 6061 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 6121 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 6181 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 6241 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 6301 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 6361 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 6421 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 6481 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 6541 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 6601 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 6661 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 6721 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 6781 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 6841 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 6901 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 6961 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 7021 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 7081 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 7141 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 7201 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 7261 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 7321 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 7381 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 7441 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 7501 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 7561 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 7621 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 7681 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 7741 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 7801 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 7861 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 7921 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 7981 agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc 8041 aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa 8101 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta 8161 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 8221 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 8281 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 8341 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 8401 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 8461 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 8521 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 8581 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 8641 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 8701 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 8761 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 8821 tcgtttagtg aaccgtcaga tcctcactct cttccgcatc gctgtctgcg agggccagct 8881 gttgggctcg cggttgagga caaactcttc gcggtctttc cagtactctt ggatcggaaa 8941 cccgtcggcc tccgaacggt actccgccac cgagggacct gagcgagtcc gcatcgaccg 9001 gatcggaaaa cct //