LOCUS Exported 9154 bp ds-DNA circular SYN 12-JUN-2013 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled1 - Sequence SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9154) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 19, 2018 from SnapGene Viewer 4.1.8 http://www.snapgene.com COMMENT New NA Sequence FEATURES Location/Qualifiers source 1..9154 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..172 /label=TPL2/3 /note="TPL2/3" misc_feature 154..156 /label=SD /note="SD" promoter 181..331 /note="MLP" misc_feature 339..341 /label=SA /note="SA" CDS 352..408 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /note="residues deleted (2012-01-10)" /translation="ATMEFQTQVLMSLLLCMSG" CDS 409..416 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /translation="AA" CDS 417..1911 /codon_start=1 /label=TRAPblac /translation="HNGPSGQREVPRDRVPDLLRPVPRERPGRAEQHRGRDQVPRGSVQ RRGGPVPADGLQRQHPAAQLGQPCRASGHEADPAAEPERQRHPPVRQRVQQQRQRDHQT AQRRQQEQREGPDHHQEPAEHQPALRQDQPGRCSAASAEAPE*PDQQRERQSAGRDPDR RHPRQHPGQPEREPEAERPGREDRRVWHRTGHQRGLQPVPRGLPP*RRQVQSVRRQRLG EREECDRPFHEGRVRGSGKDRQLRRVG*VVPLQRDLRQGHQKPEERDPARGLHQRAAGT VRRGTVPAQEGTPGCARRARGRSA*TQRRQLRRGKAQREHHRQQPPGTQPQPRRGKGRE PQRLRSGREP*EPPQPSQSP*PPKSTKSSQP*HPRAGT*HP*GQRERGAQRRAQEPRG* PGRELRHPQEAREQARQPEQPA*RQVRPVHPLQPPEPQGAGQREKAERPPEPGQQRQQT RAKQRGQRDTAPRPEQRESGLQPEAQQCPQAP*AGRTRKT" CDS 1920..2468 /codon_start=1 /label=AscI CD4d3+4 /note="AscI CD4d3+4" /note="residues deleted (2006-03-14)" /translation="VDLHHGL*E*GGVSGVLLPTQPWRGKPAGRVEMEGREGSFFPVLD HLLPKEPKGVCAEVY*QPQVPAVRNAPTHPSDTPGLPSVCWFWQPDPDSGQRDTVSGSE PGGDESDSARQQHFDL*GDGTHLTQDETDLEAGESGGQGLQAGESDSSAGP*SRGVAMS TE*R*RGQDGLQDPGFIQRV" misc_feature complement(2140..2159) misc_feature 2140..2159 CDS 2469..3471 /codon_start=1 /label=L1 - COMP - L2 - Blac /note="L1 - COMP - L2 - Blac" /note="residues deleted (2010-08-05)" /translation="EFRRRLRRRYRWRRPSPTDASRTPGD*CGAARRERALATAGQGDH LPEEYGDGM*RLRSRQKPASTSTSGPTPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP* EFSPRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWH DSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKR RA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**I WSR*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*AL" misc_feature 3472..3584 /label=Flag-His /note="Flag-His" 3'UTR 3598..3692 /label=polyA /note="polyA" misc_feature complement(3760..3779) rep_origin 3989..5855 /label=OriP /note="OriP" CDS 6087..6947 /codon_start=1 /label=AmpR /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 7092..7765 /label=pUC origin /note="pUC origin" promoter 8395..8983 /note="CMV promoter" TATA_signal 8948..8953 /note="TATA box" misc_feature 8987..9006 /label=TPL1 /note="TPL1" misc_feature 9024..9154 /label=TPL2/3 /note="TPL2/3" ORIGIN 1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg 61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta 121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt 181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca 241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg 301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg 361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccaca 421 atggcccatc tgggcaacgt gaagtacctc gtgatcgtgt tcctgatctt cttcgacctg 481 ttcctcgtga acggccggga cgtgcagaac aacatcgtgg acgagatcaa gtaccgcgag 541 gaagtgtgca acgacgaggt ggacctgtac ctgctgatgg actgcagcgg cagcatccgg 601 cggcacaact gggtcaacca tgccgtgcct ctggccatga agctgatcca gcagctgaac 661 ctgaacgaca acgccatcca cctgtacgcc agcgtgttca gcaacaacgc cagagagatc 721 atcagactgc acagcgacgc cagcaagaac aaagagaagg ccctgatcat catcaagagc 781 ctgctgagca ccaacctgcc ctacggcaag accaacctgg ccgatgctct gctgcaagtg 841 cggaagcacc tgaatgaccg gatcaacaga gagaacgcca atcagctggt cgtgatcctg 901 accgacggca tccccgacag catccaggac agcctgaaag agagccggaa gctgagcgac 961 cggggcgtga agatcgccgt gtttggcatc ggacagggca tcaacgtggc cttcaaccgg 1021 ttcctcgtgg gctgccaccc tagcgacggc aagtgcaatc tgtacgccga cagcgcctgg 1081 gagaacgtga agaatgtgat cggccctttc atgaaggccg tgtgcgtgga agtggaaaag 1141 accgccagct gcggcgtgtg ggatgagtgg tccccttgca gcgtgacctg cggcaagggc 1201 accagaagcc ggaagagaga gatcctgcac gagggctgca ccagcgagct gcaggaacag 1261 tgcgaagagg aacggtgcct gcccaagagg gaacccctgg atgtgcccga cgagcccgag 1321 gacgatcagc ctagacccag aggcgacaac ttcgccgtgg aaaagcccaa cgagaacatc 1381 atcgacaaca acccccagga acccagcccc aaccccgaag agggaaaggg cgagaacccc 1441 aacggcttcg atctggacga gaaccctgag aaccccccca accctcccaa tccccctaac 1501 cccccaaatc caccaaatcc tcccaaccct gacatccccg agcaggaacc taacatccct 1561 gaggacagcg agaaagaggt gcccagcgac gtgcccaaga accccgagga tgaccgggaa 1621 gagaacttcg acatccccaa gaagccagag aacaagcacg acaaccagaa caacctgcct 1681 aacgacaagt ccgaccggta catcccctac agccccctga gccccaaggt gctggacaac 1741 gagagaaagc agagcgaccc ccagagccag gacaacaacg gcaacagaca cgtgccaaac 1801 agcgaggaca gagagacacg gccccacggc cggaacaacg agaatcgggc ctacaaccgg 1861 aagcacaaca atgcccccaa gcaccctgag cgggaagaac acgaaaaacc tggcgcgccg 1921 tcgacctcca tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc 1981 aaccttggag aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct 2041 tcccagtcct ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact 2101 agcaacccca agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc 2161 tcccttcagt ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat 2221 caggaagtga acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt 2281 gaggtgatgg gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag 2341 gccagggtct ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg 2401 caatgtctac tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc 2461 aaagggttga attccggcgg cggctccgga ggcggtaccg gtggcggcga cctagcccca 2521 cagatgcttc gagaactcca ggagactaat gcggcgctgc aagacgtgag agagctcttg 2581 cgacagcagg tcaaggagat caccttcctg aagaatacgg tgatggaatg tgacgcctgc 2641 ggtctagaca gaaacctgcc tccactagca cctctgggcc cacacccaga aacgctggtg 2701 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 2761 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 2821 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 2881 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 2941 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 3001 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 3061 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 3121 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 3181 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 3241 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 3301 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 3361 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 3421 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gggccctgac 3481 tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga tgacgatgac 3541 aagggccctc accaccacca tcaccactaa ttaattaagg atcccccgac ctcgacctct 3601 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca 3661 ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag atctctagct 3721 agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt 3781 cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt 3841 tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc 3901 cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt 3961 gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta 4021 caccaatgct gggggacatg tacctcccag gggcccagga agactacggg aggctacacc 4081 aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc 4141 aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag 4201 tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat 4261 atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat 4321 gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta 4381 gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc 4441 tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg 4501 tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg 4561 gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc 4621 aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg 4681 gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac 4741 acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa 4801 gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga 4861 cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc 4921 aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg 4981 cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa 5041 cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta 5101 atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg 5161 ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg 5221 gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata 5281 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcataggct 5341 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct 5401 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct 5461 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct 5521 atcctaatag agattagggt agtatatgct atcctaattt atatctgggt agcatatact 5581 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc 5641 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc 5701 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc 5761 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc 5821 taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca tgagaatttt 5881 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 5941 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 6001 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 6061 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 6121 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 6181 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 6241 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 6301 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc 6361 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 6421 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 6481 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 6541 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 6601 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat 6661 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 6721 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 6781 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 6841 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 6901 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 6961 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 7021 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 7081 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 7141 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 7201 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 7261 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 7321 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 7381 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 7441 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 7501 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 7561 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 7621 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 7681 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 7741 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 7801 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 7861 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 7921 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 7981 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 8041 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 8101 gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca tgtttgacag 8161 cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga actggtaggt 8221 atggaagatc tatacattga atcaatattg gcaattagcc atattagtca ttggttatat 8281 agcataaatc aatattggct attggccatt gcatacgttg tatctatatc ataatatgta 8341 catttatatt ggctcatgtc caatatgacc gccatgttga cattgattat tgactagtta 8401 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 8461 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 8521 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 8581 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc 8641 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 8701 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 8761 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc 8821 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt 8881 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg 8941 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcctcactc tcttccgcat 9001 cgctgtctgc gagggccagc tgttgggctc gcggttgagg acaaactctt cgcggtcttt 9061 ccagtactct tggatcggaa acccgtcggc ctccgaacgg tactccgcca ccgagggacc 9121 tgagcgagtc cgcatcgacc ggatcggaaa acct //