LOCUS       Exported                8293 bp ds-DNA     circular SYN 12-JUN-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled1 - Sequence
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 19, 2018 from SnapGene Viewer 4.1.8
            http://www.snapgene.com
COMMENT     New NA Sequence
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..172
                     /label=TPL2/3
                     /note="TPL2/3"
     misc_feature    154..156
                     /label=SD
                     /note="SD"
     promoter        181..331
                     /note="MLP"
     misc_feature    339..341
                     /label=SA
                     /note="SA"
     CDS             352..408
                     /codon_start=1
                     /label=Kozak+leader Vkappa 7-33
                     /note="Kozak+leader Vkappa 7-33"
                     /note="residues deleted (2012-01-10)"
                     /translation="ATMEFQTQVLMSLLLCMSG"
     CDS             409..416
                     /codon_start=1
                     /label=Kozak+leader Vkappa 7-33
                     /note="Kozak+leader Vkappa 7-33"
                     /translation="AA"
     CDS             417..1050
                     /codon_start=1
                     /label=PF3D7_1351800_1
                     /note="residues deleted (2014-06-12)"
                     /translation="RERAEQRLPQAGRGQIPVRPEPDEVPGHREEQQRTEQQDGHPGQQ
                     AVHHLPVPQQHLPVLQQSDLHAGKQRQRRDHQVQRKQQDRGLRQVHQAHRGAAEEHQQR
                     EQPRAEHLLRGTEAEGQEGAGPRPGSEQHHPVRERRDY*VLQEAAAESLQRRQEQPAEP
                     GQHQERPEEKQRRLRQRLR**RQQR*Q*R*RR*QL*QQQ**WLRLPHQ"
     CDS             1059..1607
                     /codon_start=1
                     /label=AscI CD4d3+4
                     /note="AscI CD4d3+4"
                     /note="residues deleted (2006-03-14)"
                     /translation="VDLHHGL*E*GGVSGVLLPTQPWRGKPAGRVEMEGREGSFFPVLD
                     HLLPKEPKGVCAEVY*QPQVPAVRNAPTHPSDTPGLPSVCWFWQPDPDSGQRDTVSGSE
                     PGGDESDSARQQHFDL*GDGTHLTQDETDLEAGESGGQGLQAGESDSSAGP*SRGVAMS
                     TE*R*RGQDGLQDPGFIQRV"
     misc_feature    complement(1279..1298)
     misc_feature    1279..1298
     CDS             1608..2610
                     /codon_start=1
                     /label=L1 - COMP - L2 - Blac
                     /note="L1 - COMP - L2 - Blac"
                     /note="residues deleted (2010-08-05)"
                     /translation="EFRRRLRRRYRWRRPSPTDASRTPGD*CGAARRERALATAGQGDH
                     LPEEYGDGM*RLRSRQKPASTSTSGPTPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP*
                     EFSPRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWH
                     DSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKR
                     RA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**I
                     WSR*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*AL"
     misc_feature    2611..2723
                     /label=Flag-His
                     /note="Flag-His"
     3'UTR           2737..2831
                     /label=polyA
                     /note="polyA"
     misc_feature    complement(2899..2918)
     rep_origin      3128..4994
                     /label=OriP
                     /note="OriP"
     CDS             5226..6086
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    6231..6904
                     /label=pUC origin
                     /note="pUC origin"
     promoter        7534..8122
                     /note="CMV promoter"
     TATA_signal     8087..8092
                     /note="TATA box"
     misc_feature    8126..8145
                     /label=TPL1
                     /note="TPL1"
     misc_feature    8163..8293
                     /label=TPL2/3
                     /note="TPL2/3"
ORIGIN
        1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg
       61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta
      121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt
      181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca
      241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg
      301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg
      361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccgtg
      421 aaagagcgga acaacggctt ccccaagcgg ggcgagggca gatacctgtt cggccggaac
      481 cagatgaagt cccaggacat cgagaagaac aacaaagaac tgagcaacaa gatggacacc
      541 caggacagca agcagtacat cacctacctg taccacagca gcatctgcca gtactgcagc
      601 aaagtgacct ccatgctgga aaacaacgac aacgtcgaga tcatcaagtt caaagaaaac
      661 aacaagatcg aggacttcgg caagttcacc aagcccatcg tggtgctgct gaagaacatc
      721 aacaaagaga acagcctcga gcggagcatc ttctacgagg aactgaagcg gaagggcaag
      781 aaggtgcagg tccccgccct ggaagtgaac aacatcatcc tgttcgagag cgacgagatt
      841 attaagttct acaagaagct gctgcagaaa gtctccaacg acgacaagag cagcctgcag
      901 aaccggggca acatcaagaa cgaccagaag aaaaacaacg ccgactacga caacgactac
      961 gataatgacg acaacaacga tgacaatgac gatgacgacg ataacaacta taacaacaac
     1021 aatgatgatg gctacgacta ccacaccagc ggcgcgccgt cgacctccat cacggcctat
     1081 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
     1141 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
     1201 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
     1261 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
     1321 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
     1381 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
     1441 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
     1501 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
     1561 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttccggcggc
     1621 ggctccggag gcggtaccgg tggcggcgac ctagccccac agatgcttcg agaactccag
     1681 gagactaatg cggcgctgca agacgtgaga gagctcttgc gacagcaggt caaggagatc
     1741 accttcctga agaatacggt gatggaatgt gacgcctgcg gtctagacag aaacctgcct
     1801 ccactagcac ctctgggccc acacccagaa acgctggtga aagtaaaaga tgctgaagat
     1861 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1921 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1981 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     2041 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     2101 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     2161 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     2221 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2281 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2341 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2401 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2461 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2521 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2581 gagataggtg cctcactgat taagcattgg ggccctgact acaaagacca tgacggtgat
     2641 tataaagatc atgacatcga ttacaaggat gacgatgaca agggccctca ccaccaccat
     2701 caccactaat taattaagga tcccccgacc tcgacctctg gctaataaag gaaatttatt
     2761 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     2821 gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat ccccgccccg
     2881 gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc
     2941 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
     3001 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
     3061 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
     3121 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
     3181 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
     3241 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
     3301 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
     3361 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
     3421 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
     3481 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
     3541 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
     3601 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
     3661 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
     3721 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
     3781 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
     3841 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     3901 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     3961 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     4021 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     4081 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     4141 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     4201 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     4261 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     4321 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     4381 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     4441 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     4501 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     4561 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4621 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     4681 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     4741 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     4801 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     4861 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     4921 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     4981 atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga aagggcctcg
     5041 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5101 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5161 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5221 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5281 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5341 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5401 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5461 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5521 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5581 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5641 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5701 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5761 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5821 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5881 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5941 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6001 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6061 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6121 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6181 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6241 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6301 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6361 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6421 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6481 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6541 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6601 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6661 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6721 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6781 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6841 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6901 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6961 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7021 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7081 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7141 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7201 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7261 agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc
     7321 aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa
     7381 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
     7441 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
     7501 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     7561 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     7621 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     7681 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     7741 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
     7801 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     7861 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     7921 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     7981 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     8041 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     8101 tcgtttagtg aaccgtcaga tcctcactct cttccgcatc gctgtctgcg agggccagct
     8161 gttgggctcg cggttgagga caaactcttc gcggtctttc cagtactctt ggatcggaaa
     8221 cccgtcggcc tccgaacggt actccgccac cgagggacct gagcgagtcc gcatcgaccg
     8281 gatcggaaaa cct
//
