LOCUS Exported 8320 bp ds-DNA circular SYN 04-AUG-2014 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled1 - Sequence SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8320) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 19, 2018 from SnapGene Viewer 4.1.8 http://www.snapgene.com COMMENT New NA Sequence FEATURES Location/Qualifiers source 1..8320 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..172 /label=TPL2/3 /note="TPL2/3" misc_feature 154..156 /label=SD /note="SD" promoter 181..331 /note="MLP" misc_feature 339..341 /label=SA /note="SA" CDS 352..408 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /note="residues deleted (2012-01-10)" /translation="ATMEFQTQVLMSLLLCMSG" CDS 409..416 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /translation="AA" CDS 417..1077 /codon_start=1 /label=PF3D7_0721100 /note="residues deleted (2014-08-04)" /translation="QRQPDL*VRVLRQAEQGPETGVLCAHQRREHVQRDNPEAAEQRDQ PAGRTPEREQRQPQHGQQEKRGQLRGNQEERREQQPQAQRDGREAEVHREVPELRQGRA EERGIQVRECGRPEVQSEVQPVRVHRDREQQAHPAGPDHPDLHERVYVRLLLLRQHQPV HPEQ*VQQQGPQGLQQRPEAGRHLLQYRQGHQRGQRERHQFLQAAEQQVPPRVLRDR" CDS 1086..1634 /codon_start=1 /label=AscI CD4d3+4 /note="AscI CD4d3+4" /note="residues deleted (2006-03-14)" /translation="VDLHHGL*E*GGVSGVLLPTQPWRGKPAGRVEMEGREGSFFPVLD HLLPKEPKGVCAEVY*QPQVPAVRNAPTHPSDTPGLPSVCWFWQPDPDSGQRDTVSGSE PGGDESDSARQQHFDL*GDGTHLTQDETDLEAGESGGQGLQAGESDSSAGP*SRGVAMS TE*R*RGQDGLQDPGFIQRV" misc_feature complement(1306..1325) misc_feature 1306..1325 CDS 1635..2637 /codon_start=1 /label=L1 - COMP - L2 - Blac /note="L1 - COMP - L2 - Blac" /note="residues deleted (2010-08-05)" /translation="EFRRRLRRRYRWRRPSPTDASRTPGD*CGAARRERALATAGQGDH LPEEYGDGM*RLRSRQKPASTSTSGPTPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP* EFSPRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWH DSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKR RA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**I WSR*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*AL" misc_feature 2638..2750 /label=Flag-His /note="Flag-His" 3'UTR 2764..2858 /label=polyA /note="polyA" misc_feature complement(2926..2945) rep_origin 3155..5021 /label=OriP /note="OriP" CDS 5253..6113 /codon_start=1 /label=AmpR /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 6258..6931 /label=pUC origin /note="pUC origin" promoter 7561..8149 /note="CMV promoter" TATA_signal 8114..8119 /note="TATA box" misc_feature 8153..8172 /label=TPL1 /note="TPL1" misc_feature 8190..8320 /label=TPL2/3 /note="TPL2/3" ORIGIN 1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg 61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta 121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt 181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca 241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg 301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg 361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccaga 421 ggcaaccaga tctctaagtg cgtgttctac gacaagcgga acaaggacct gaaacaggtg 481 ttctatgtgc ccaccaacga cgtgaacatg tacaaagaga caatccggaa gctgctgagc 541 agagagatca gcctgctgga agaacccctg aacgagaaca gcgacagcca caacatggcc 601 aacaagaaaa acgtggccaa cttcgaggaa atcaagaaga acgacgagaa caacaaccgc 661 aagcgcaaag agatggaaga gaagctgaag tacatcgaga agtaccggaa ctacgccaag 721 ggcgtgctga agaacgtgga attcaagtgc gagaatgtgg acgacctgaa gtccaaagtg 781 aagtgcaacc tgtgcgtgta caccgagatc gtgaacaaca agcacatccg gcagggccag 841 atcatccaga tcttcatgaa cgagtgtatg tacggcttct actactacga caacatcaac 901 cagtacaccc agaacaatga gtacaacaac aaggccctca aggactacaa cagcgcccag 961 aagctggacg acatctactt caatatcgcc aaggacatca gcgagggcaa cgtgaacggc 1021 atcaatttct tcaagcagca gagcagcaag taccaccccg tgtgctacgt gaccgaaggc 1081 gcgccgtcga cctccatcac ggcctataag agtgaggggg agtcagcgga gttctccttc 1141 ccactcaacc ttggagagga aagcctgcag ggagagttga gatggaaggc agagaaggct 1201 ccttcttccc agtcctggat caccttctcc ctaaagaacc aaaaggtgtc tgtgcagaag 1261 tctactagca accccaagtt ccagctgtcc gaaacgctcc cactcaccct tcagataccc 1321 caggtctccc ttcagtttgc tggttctggc aacctgaccc tgactctgga cagagggata 1381 ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc agcccgacag caacactttg 1441 acctgtgagg tgatgggacc cacctcaccc aagatgagac tgatcttgaa gcaggagaat 1501 caggaggcca gggtctccag gcaggagaaa gtgattcaag tgcaggcccc tgaagcaggg 1561 gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga tggactccaa gatccaggtt 1621 ttatccaaag ggttgaattc cggcggcggc tccggaggcg gtaccggtgg cggcgaccta 1681 gccccacaga tgcttcgaga actccaggag actaatgcgg cgctgcaaga cgtgagagag 1741 ctcttgcgac agcaggtcaa ggagatcacc ttcctgaaga atacggtgat ggaatgtgac 1801 gcctgcggtc tagacagaaa cctgcctcca ctagcacctc tgggcccaca cccagaaacg 1861 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 1921 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 1981 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 2041 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 2101 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 2161 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 2221 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 2281 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 2341 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 2401 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 2461 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 2521 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 2581 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggggc 2641 cctgactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac 2701 gatgacaagg gccctcacca ccaccatcac cactaattaa ttaaggatcc cccgacctcg 2761 acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt 2821 ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc tcggagatct 2881 ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc 2941 cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg 3001 ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt 3061 gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca 3121 gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag 3181 ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc 3241 tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc 3301 attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc 3361 gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg 3421 aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca 3481 ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc 3541 attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc 3601 ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca 3661 gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt 3721 tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc 3781 ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc 3841 catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg 3901 ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg 3961 gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga 4021 cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc 4081 cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag 4141 tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg 4201 taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa 4261 ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg 4321 cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg 4381 ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt 4441 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca 4501 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta 4561 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca 4621 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca 4681 tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca 4741 tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg 4801 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg 4861 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg 4921 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg 4981 ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt caaacatgag 5041 aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 5101 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 5161 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 5221 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 5281 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 5341 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 5401 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 5461 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg 5521 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 5581 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 5641 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 5701 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 5761 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca 5821 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 5881 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 5941 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 6001 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 6061 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 6121 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 6181 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 6241 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 6301 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 6361 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 6421 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 6481 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 6541 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 6601 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 6661 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 6721 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 6781 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 6841 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 6901 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 6961 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 7021 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 7081 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 7141 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 7201 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 7261 gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt 7321 tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg cgcagaactg 7381 gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg 7441 ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa 7501 tatgtacatt tatattggct catgtccaat atgaccgcca tgttgacatt gattattgac 7561 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 7621 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 7681 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 7741 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 7801 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 7861 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 7921 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg 7981 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 8041 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt 8101 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcc tcactctctt 8161 ccgcatcgct gtctgcgagg gccagctgtt gggctcgcgg ttgaggacaa actcttcgcg 8221 gtctttccag tactcttgga tcggaaaccc gtcggcctcc gaacggtact ccgccaccga 8281 gggacctgag cgagtccgca tcgaccggat cggaaaacct //