LOCUS Exported 9655 bp ds-DNA circular SYN 04-AUG-2014 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled1 - Sequence SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9655) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 19, 2018 from SnapGene Viewer 4.1.8 http://www.snapgene.com COMMENT New NA Sequence FEATURES Location/Qualifiers source 1..9655 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..172 /label=TPL2/3 /note="TPL2/3" misc_feature 154..156 /label=SD /note="SD" promoter 181..331 /note="MLP" misc_feature 339..341 /label=SA /note="SA" CDS 352..408 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /note="residues deleted (2012-01-10)" /translation="ATMEFQTQVLMSLLLCMSG" CDS 409..416 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /translation="AA" CDS 417..2412 /codon_start=1 /label=PF3D7_1106200 /note="residues deleted (2014-08-04)" /translation="QQRNRHPVQQSAPEEAHLLPQKGQPLPHLQRGGQQREPQEQERVQ QQAEGPDQDLRTGAQQQVQEDLLPAPDLPEEQQ*GRVHRGPQGQEHR*Q**QQER*QEG RHQQQRQEEQNQRRPCPEYRQR*G*ELRQQLRVYREQPAEAEKQPGSVQQLYQQGRRDH RAPELPRHPEQAPDRDHPFQQHHQQPGLHLPVQQE*L*QSRHQY*QQQQQ*QQHEQVQH LPSLQQYLQYHLLHLQLQRPGQPSEHCQLHP*LFHQQKGQHYPQREVPAGPRDPEVSER PLQPQGSE*AQEVPEHQLSVHQGQYPGH*QDRQGYRREQDAVLP*EEYPQQRDKLRQHS QQR*QCQQAGRSEPDQIRPQEQ*RLL*EVQARHPEESGQRELLYL*LPEQLQAGHPQRV HLHCQGHHHPKQQCDQAHPEH*HQEEDPQYRAAAQQFQELQAQQKHQ*PRERAGNQEKA VQLPAAHHPERPAREVRQQSGNSEELPVPGVQREYPEDQD*LRYPVPV*EADQPHRPAV RLLQLQDLHPPQQQELSGPAEVLLHLLLL*DQAAAAPEPGRQ*DHQLLHQQGPVREELR LQRVQLLQEVYPDQLHHHLHL*PD*EQSEYQLQLPEPQRLRLQLLQGRYQPEP*ERHHD HHQRDGPGPENQHPQKRLHQDHADDGQGQK" CDS 2421..2969 /codon_start=1 /label=AscI CD4d3+4 /note="AscI CD4d3+4" /note="residues deleted (2006-03-14)" /translation="VDLHHGL*E*GGVSGVLLPTQPWRGKPAGRVEMEGREGSFFPVLD HLLPKEPKGVCAEVY*QPQVPAVRNAPTHPSDTPGLPSVCWFWQPDPDSGQRDTVSGSE PGGDESDSARQQHFDL*GDGTHLTQDETDLEAGESGGQGLQAGESDSSAGP*SRGVAMS TE*R*RGQDGLQDPGFIQRV" misc_feature complement(2641..2660) misc_feature 2641..2660 CDS 2970..3972 /codon_start=1 /label=L1 - COMP - L2 - Blac /note="L1 - COMP - L2 - Blac" /note="residues deleted (2010-08-05)" /translation="EFRRRLRRRYRWRRPSPTDASRTPGD*CGAARRERALATAGQGDH LPEEYGDGM*RLRSRQKPASTSTSGPTPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP* EFSPRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWH DSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKR RA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**I WSR*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*AL" misc_feature 3973..4085 /label=Flag-His /note="Flag-His" 3'UTR 4099..4193 /label=polyA /note="polyA" misc_feature complement(4261..4280) rep_origin 4490..6356 /label=OriP /note="OriP" CDS 6588..7448 /codon_start=1 /label=AmpR /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 7593..8266 /label=pUC origin /note="pUC origin" promoter 8896..9484 /note="CMV promoter" TATA_signal 9449..9454 /note="TATA box" misc_feature 9488..9507 /label=TPL1 /note="TPL1" misc_feature 9525..9655 /label=TPL2/3 /note="TPL2/3" ORIGIN 1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg 61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta 121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt 181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca 241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg 301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg 361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccaac 421 aaagaaatcg ccatcctgtg cagcaaagtg cacctgaaga agcacatcta ctaccccaga 481 aagggcagcc acttccacat ctacagcgtg gtggacaaca acgtgaacct caagaacaag 541 aacgagtaca acagcaagct gaaggaccgg atcaagatct acgaacaggt gcacaacaac 601 aagtgcaaga agatctactt cctgcccctg atcttcctga agaacaacaa taaggacgtg 661 tacatcgagg accacaagga caagaacatc gataacaata ataacaacaa gaaagataac 721 aagaaggacg acatcaacaa caacgacaag aagaacaaaa tcaacgacgc ccctgcccag 781 aatatcgcca acgataagga taagaactac gccaacaact tcgagtatat cgagaacaac 841 ctgctgaagc tgaaaaacaa cctggaagtg ttcagcagct atatcaacaa ggccgacgtg 901 atcatcgtgc ccctgaacta ccacgacatc ctgaacaagc acctgatcga gatcatccct 961 ttcaacaaca tcatcaacag cctggactac atcttcctgt tcaacaagaa tgactataac 1021 aaagccgaca tcaatattaa caacaacaac aacaataaca acaacatgaa caagtacaac 1081 atctgccctc cctacaacaa tatctacaat atcatcttct tcatctacaa ctacaacgac 1141 caggacaacc ctctgaacat tgccagctac acccctagct ttttcaccaa caaaaaggac 1201 aacattatcc acaaagagaa gttccagctg gtccacgtga tccagaagtt tctgaacgac 1261 cactacaacc tcaaggatca gaatgagcac aagaagtacc tgaacatcaa ctttctgttc 1321 accaaggaca atatcctgga cattaacaag atcgacaagg atatcgaaga gaacaagatg 1381 ctgtactacc ctaagaagaa tatcctcaac aaagagacaa actacgtcaa cacagccaac 1441 aacgctaaca atgccaacaa gcaggaagat cagaacctga tcaaatacgt ccacaagaac 1501 aatgacgcct tctttaagaa gtacaagccc gacaccctga agaatctggc caacgagaac 1561 tactttatct ataacttcct gagcagctac aagctggaca tcctcaaaga gtacatctac 1621 attgccaagg acatcaccat cccaaacaac aatgtgatca agcacatcca gaacattaac 1681 atcaagaaga agatccacaa tatcgagctg ctgctcaaca atttcaagaa ctgcaagctc 1741 aacaaaaaca tcaatagcca cgtgaacgtg caggaaatca agaaaaagca gtacagctac 1801 ctgctgcaca tcatcctgag cgacctgctc gagaagtacg acagcaatct ggaaattctg 1861 aagaactacc tgtaccaggt gttcaaagag aatatccaga agatcaagat taactccgat 1921 atcctgttcc agtttaagaa gcagatcaac cacatcgacc agctgttcga ctactacaac 1981 tccaagatct tcatcctccc cagcaacaag aactatcagg cccagcagaa gtcctactac 2041 atctattact actataagat caagctgctg cagctcctga accaggccgc caatgagatc 2101 atcaattact acatcagcaa gggcctgtac gtgaagaatt acgccttcaa cgagttcagc 2161 ttctccaaga agtataccct gatcaattac atcatcacct acatctttaa cctgattaag 2221 aacaaagtga atatcaactt caactacctg agccccaacg ccttcggctt cagctcctac 2281 aaggacgata tcagcctgag ccctaagaaa gacatcatga tcaccaccag cgagatggac 2341 caggtccaga aaatcaacat ccccagaagc gcctacacca agaccatgct gatgatggac 2401 aaggacagaa acggcgcgcc gtcgacctcc atcacggcct ataagagtga gggggagtca 2461 gcggagttct ccttcccact caaccttgga gaggaaagcc tgcagggaga gttgagatgg 2521 aaggcagaga aggctccttc ttcccagtcc tggatcacct tctccctaaa gaaccaaaag 2581 gtgtctgtgc agaagtctac tagcaacccc aagttccagc tgtccgaaac gctcccactc 2641 acccttcaga taccccaggt ctcccttcag tttgctggtt ctggcaacct gaccctgact 2701 ctggacagag ggatactgta tcaggaagtg aacctggtgg tgatgaaagt gactcagccc 2761 gacagcaaca ctttgacctg tgaggtgatg ggacccacct cacccaagat gagactgatc 2821 ttgaagcagg agaatcagga ggccagggtc tccaggcagg agaaagtgat tcaagtgcag 2881 gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag gtgaagaggt caagatggac 2941 tccaagatcc aggttttatc caaagggttg aattccggcg gcggctccgg aggcggtacc 3001 ggtggcggcg acctagcccc acagatgctt cgagaactcc aggagactaa tgcggcgctg 3061 caagacgtga gagagctctt gcgacagcag gtcaaggaga tcaccttcct gaagaatacg 3121 gtgatggaat gtgacgcctg cggtctagac agaaacctgc ctccactagc acctctgggc 3181 ccacacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 3241 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 3301 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 3361 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 3421 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 3481 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 3541 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 3601 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 3661 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 3721 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 3781 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 3841 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 3901 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 3961 attaagcatt ggggccctga ctacaaagac catgacggtg attataaaga tcatgacatc 4021 gattacaagg atgacgatga caagggccct caccaccacc atcaccacta attaattaag 4081 gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc aatagtgtgt 4141 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttggtcgag 4201 atccctcgga gatctctagc tagaggatcg atccccgccc cggacgaact aaacctgact 4261 acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt tggttggtac 4321 aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac acaaaggggt 4381 tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac actgagtggc 4441 tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc ctttatgtgt 4501 aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg 4561 aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg 4621 gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg 4681 tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat 4741 atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa 4801 cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca 4861 cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt 4921 gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact 4981 gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc 5041 ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata 5101 accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct 5161 ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac 5221 gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga 5281 tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag 5341 gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa 5401 aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt 5461 tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac 5521 tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac 5581 cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg 5641 gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga 5701 gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct 5761 aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc 5821 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc 5881 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc 5941 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc 6001 tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt 6061 tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata 6121 tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata 6181 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata 6241 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata 6301 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgataa 6361 gctgtcaaac atgagaattt tcttgaagac gaaagggcct cgtgatacgc ctatttttat 6421 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 6481 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 6541 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 6601 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 6661 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 6721 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 6781 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg 6841 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 6901 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 6961 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 7021 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 7081 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg 7141 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 7201 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 7261 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 7321 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 7381 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 7441 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 7501 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 7561 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 7621 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 7681 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 7741 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 7801 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 7861 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 7921 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 7981 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 8041 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 8101 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 8161 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 8221 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 8281 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 8341 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 8401 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 8461 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 8521 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 8581 aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg 8641 accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct 8701 gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc 8761 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt 8821 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg 8881 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc 8941 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 9001 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 9061 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 9121 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 9181 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt 9241 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg 9301 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 9361 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat 9421 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca 9481 gatcctcact ctcttccgca tcgctgtctg cgagggccag ctgttgggct cgcggttgag 9541 gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg cctccgaacg 9601 gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa aacct //