LOCUS Exported 8344 bp ds-DNA circular SYN 04-AUG-2014 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled1 - Sequence SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8344) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 19, 2018 from SnapGene Viewer 4.1.8 http://www.snapgene.com COMMENT New NA Sequence FEATURES Location/Qualifiers source 1..8344 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..172 /label=TPL2/3 /note="TPL2/3" misc_feature 154..156 /label=SD /note="SD" promoter 181..331 /note="MLP" misc_feature 339..341 /label=SA /note="SA" CDS 352..408 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /note="residues deleted (2012-01-10)" /translation="ATMEFQTQVLMSLLLCMSG" CDS 409..416 /codon_start=1 /label=Kozak+leader Vkappa 7-33 /note="Kozak+leader Vkappa 7-33" /translation="AA" CDS 417..1101 /codon_start=1 /label=PF3D7_1449000 /note="residues deleted (2014-08-04)" /translation="HSAAQRELRPSELHRDRRHPEQQSGRAVAQPPEQLHRHRVHQGGQ QAGR*PVQRQQPREVHDPARGRRRHLRRQRLRGQEPGPDPDRVHPEAEGQVQEQQVRPE AEKAGQQGQREAAEPVPEAQGQAAALLQHPAEVPGHPYRRAVHQKEPEQVEAADPGSHT EAGRAEQEYRPAHHQPAVQQLRGQDRRVQPGKQDQRRG*AERPGPAGQGQAGHPETRGT GKGSAA" CDS 1110..1658 /codon_start=1 /label=AscI CD4d3+4 /note="AscI CD4d3+4" /note="residues deleted (2006-03-14)" /translation="VDLHHGL*E*GGVSGVLLPTQPWRGKPAGRVEMEGREGSFFPVLD HLLPKEPKGVCAEVY*QPQVPAVRNAPTHPSDTPGLPSVCWFWQPDPDSGQRDTVSGSE PGGDESDSARQQHFDL*GDGTHLTQDETDLEAGESGGQGLQAGESDSSAGP*SRGVAMS TE*R*RGQDGLQDPGFIQRV" misc_feature complement(1330..1349) misc_feature 1330..1349 CDS 1659..2661 /codon_start=1 /label=L1 - COMP - L2 - Blac /note="L1 - COMP - L2 - Blac" /note="residues deleted (2010-08-05)" /translation="EFRRRLRRRYRWRRPSPTDASRTPGD*CGAARRERALATAGQGDH LPEEYGDGM*RLRSRQKPASTSTSGPTPRNAGESKRC*RSVGCTSGLHRTGSQQR*DP* EFSPRRTFSNDEHF*SSAMWRGIIPY*RRARATRSPHTLFSE*LG*VLTSHRKASYGWH DSKRIMQCCHNHE**HCGQLTSDNDRRTEGANRFFAQHGGSCNSP*SLGTGAE*SHTKR RA*HHDACSNGNNVAQTINWRTTYSSFPATINRLDGGG*SCRTTSALGPSGWLVYC**I WSR*AWVSRYHCSTGARW*ALPYRSYLHDGESGNYG*TK*TDR*DRCLTD*AL" misc_feature 2662..2774 /label=Flag-His /note="Flag-His" 3'UTR 2788..2882 /label=polyA /note="polyA" misc_feature complement(2950..2969) rep_origin 3179..5045 /label=OriP /note="OriP" CDS 5277..6137 /codon_start=1 /label=AmpR /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 6282..6955 /label=pUC origin /note="pUC origin" promoter 7585..8173 /note="CMV promoter" TATA_signal 8138..8143 /note="TATA box" misc_feature 8177..8196 /label=TPL1 /note="TPL1" misc_feature 8214..8344 /label=TPL2/3 /note="TPL2/3" ORIGIN 1 ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg 61 cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt aattaaagta 121 ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc aggcttgaga tccagctgtt 181 ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg tcagtttcca 241 aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg agtgacaatg 301 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaagtt tgccaccatg 361 gagtttcaga cccaggtact catgtccctg ctgctctgca tgtctggtgc ggccgccatt 421 cagctgcaca gcgtgaacta cgcccctctg aactacatcg agatcggcga caccctgagc 481 aacaaagtgg gcgagctgtg gcacagccac ctgaacagct tcatcgacat cgtgtccacc 541 aaggtggtca acaagctgga agatgacctg tccaacggca acaacctcga gaagttcatg 601 atcctgctcg aggacgacgc cgacatcttc gacgccagcg cctacgaggg caagaacctg 661 gccctgatcc agaccgagtt catccggaag ctgaaggaca agttcaagaa cagcaagttc 721 ggccagaagc tgaaaaagct gggcagcaag gccaaagaga agctgctgag cctgtaccag 781 aagcacaagg gcaagctgcg gcacttcttc agcatcctgc tgaagtccct ggtcatccct 841 atcgccgtgc agttcatcag aaagaacctg aacaagtgga agcagcggac cctggaagcc 901 acacagaagc tggacgagca gagcaagaat atcgcccagc ccatcatcaa ccggctgtac 961 aacagcttcg aggacaagat cgaagagtac agccaggaaa acaagatcaa cgtcgaggat 1021 gagctgaacg ccctgggcca gctggacaag gacaagcagg acatccagaa actcgaggaa 1081 caggaaaagg ctctgctgca gggcgcgccg tcgacctcca tcacggccta taagagtgag 1141 ggggagtcag cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag 1201 ttgagatgga aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag 1261 aaccaaaagg tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg 1321 ctcccactca cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg 1381 accctgactc tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg 1441 actcagcccg acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg 1501 agactgatct tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt 1561 caagtgcagg cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc 1621 aagatggact ccaagatcca ggttttatcc aaagggttga attccggcgg cggctccgga 1681 ggcggtaccg gtggcggcga cctagcccca cagatgcttc gagaactcca ggagactaat 1741 gcggcgctgc aagacgtgag agagctcttg cgacagcagg tcaaggagat caccttcctg 1801 aagaatacgg tgatggaatg tgacgcctgc ggtctagaca gaaacctgcc tccactagca 1861 cctctgggcc cacacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 1921 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 1981 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 2041 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 2101 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 2161 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 2221 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 2281 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 2341 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 2401 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 2461 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 2521 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 2581 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 2641 gcctcactga ttaagcattg gggccctgac tacaaagacc atgacggtga ttataaagat 2701 catgacatcg attacaagga tgacgatgac aagggccctc accaccacca tcaccactaa 2761 ttaattaagg atcccccgac ctcgacctct ggctaataaa ggaaatttat tttcattgca 2821 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat 2881 ttggtcgaga tccctcggag atctctagct agaggatcga tccccgcccc ggacgaacta 2941 aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat cccttcagtt 3001 ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg ggaccaaaca 3061 caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca tttgccaaca 3121 ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca caacattgcc 3181 tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg tacctcccag 3241 gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct gtgtagctac 3301 cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc cttgttaacc 3361 ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact aaccctaatt 3421 caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta gtaaaagggt 3481 cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt acatggggtc 3541 aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga agatcaagga 3601 gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg tttagctaat 3661 agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc 3721 aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt gtgctatgac 3781 accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa tgaaacattc 3841 tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga ctggatgtcc 3901 atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat gatactgggg 3961 ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt acactctatt 4021 tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt tgtctctaac 4081 acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag acaagtggcc 4141 actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg ccgccctgcg 4201 gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg ctaaccactg 4261 cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc tgcataagta 4321 ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat tacacacact 4381 tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca 4441 cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat agcatatgct 4501 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct 4561 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct 4621 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct 4681 atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct 4741 atcctaattt atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc 4801 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc 4861 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc 4921 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc 4981 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc 5041 tcatgataag ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc gtgatacgcc 5101 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 5161 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 5221 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 5281 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 5341 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 5401 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 5461 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg 5521 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 5581 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 5641 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 5701 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 5761 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 5821 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 5881 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 5941 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 6001 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 6061 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 6121 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 6181 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 6241 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 6301 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 6361 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 6421 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 6481 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 6541 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 6601 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 6661 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 6721 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 6781 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 6841 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 6901 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 6961 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 7021 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 7081 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 7141 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 7201 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 7261 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc 7321 tagaggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg caacgttgtt 7381 gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga atcaatattg 7441 gcaattagcc atattagtca ttggttatat agcataaatc aatattggct attggccatt 7501 gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc 7561 gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 7621 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 7681 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 7741 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 7801 agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg 7861 gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat 7921 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca ccaatgggcg 7981 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 8041 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc ccgccccgtt 8101 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt 8161 gaaccgtcag atcctcactc tcttccgcat cgctgtctgc gagggccagc tgttgggctc 8221 gcggttgagg acaaactctt cgcggtcttt ccagtactct tggatcggaa acccgtcggc 8281 ctccgaacgg tactccgcca ccgagggacc tgagcgagtc cgcatcgacc ggatcggaaa 8341 acct //