LOCUS       Exported                6026 bp ds-DNA     circular SYN 20-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCMV_dLight1.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 20, 2018 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     Ligation of : Nb80-GFP 6HIS [3950 nt] : (NotI[1127] / HindIII[5082])
            to Anacy2551-g3_Geneblock [636 nt] : (HindIII[4] / NotI[643])
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          258..1017
                     /note="source [*]"
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..2185"
     source          1021..1029
                     /note="source [5']"
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..1351"
     source          1774..2336
                     /note="source [*]"
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..2185"
     promoter        1..204
                     /note="CMV promoter_promoter"
                     /note="note=""CMV promoter"" - 365..568"
     CDS             277..2322
                     /codon_start=1
                     /note="dLight1.1"
                     /translation="MKTIIALSYIFCLVFADYKDDDDAMRTLNTSAMDGTGLVVERDFS
                     VRILTACFLSLLILSTLLGNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPW
                     KAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVDRYWAISSPFRYERKMTPKA
                     AFILISVAWTLSVLISFIPVQLSWHKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISS
                     SVISFYIPVAIMIVTYTRIYRIAQKQLSSLINVYIKADKQKNGIKANFKIRHNIEDGGV
                     QLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDE
                     LYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
                     TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKT
                     RAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNNHDQLKRETKVLKTLSVIMGVFV
                     CCWLPFFILNCILPFCGSGETQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAF
                     STLLGCYRLCPATNNAIETVSINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSED
                     LKKEEAAGIARPLEKLSPALSVILDYDTDVSLEKIQPITQNGQHPT"
     misc_feature    277..324
                     /note="HA secretory sequence_misc_feature"
                     /note="note=""HA secretory sequence"" - 23..70"
     CDS             325..348
                     /note="FLAG_CDS"
                     /note="codon_start=1 - note=""FLAG"" - 
                     translation=""DYKDDDDA"" - 71..94"
     CDS             325..345
                     /codon_start=1
                     /note="Flag-tag"
                     /translation="DYKDDDD"
     CDS             349..1017
                     /note="DRD1_CDS [*]"
                     /note="codon_start=1 - note=""DRD1"" - 
                     translation=""MRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVC
                      - 
                     AAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDI
                      - 
                     MCSTASILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSW
                      - HKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISSSVISF"
     misc_feature    925..1002
                     /note="TM5_misc_feature"
                     /note="note=""TM5"" - 671..748"
     CDS             1774..2322
                     /note="DRD1_CDS [*]"
                     /note="codon_start=1 - note=""DRD1"" - 
                     translation=""MRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVC
                      - 
                     AAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDI
                      - 
                     MCSTASILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSW
                      - HKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISSSVISF"
     CDS             1990..2016
                     /note="hydrophobic ER export motif_CDS"
                     /note="codon_start=1 - note=""hydrophobic ER export motif""
                     - translation=""FRKAFSTLL"" - 1838..1864"
     polyA_signal    2450..2571
                     /note="SV40 poly(A) signal_polyA_signal"
                     /note="note=""SV40 poly(A) signal"" - 1884..2005"
     rep_origin      complement(2578..3033)
                     /direction=LEFT
                     /note="f1 ori_rep_origin"
                     /note="direction=LEFT - note=""f1 ori"" - 
                     complement(2012..2467)"
     promoter        3060..3164
                     /note="AmpR promoter_promoter"
                     /note="note=""AmpR promoter"" - 2494..2598"
     promoter        3166..3523
                     /note="SV40 promoter_promoter"
                     /note="note=""SV40 promoter"" - 2600..2957"
     rep_origin      3374..3509
                     /direction=RIGHT
                     /note="SV40 ori_rep_origin"
                     /note="note=""SV40 ori"" - 2808..2943"
     CDS             3558..4352
                     /note="NeoR/KanR_CDS"
                     /note="codon_start=1 - note=""NeoR/KanR"" - 
                     translation=""MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                      - 
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                      - 
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                      - GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMV"
     misc_feature    4584..4631
                     /note="HSV TK poly(A) signal_misc_feature"
                     /note="note=""HSV TK poly(A) signal"" - 4018..4065"
     rep_origin      4960..5548
                     /direction=RIGHT
                     /note="ori_rep_origin"
                     /note="direction=RIGHT - note=""ori"" - 4394..4982"
     enhancer        5723..6026
                     /note="CMV enhancer_enhancer"
                     /note="note=""CMV enhancer"" - 61..364"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttaccggtg cgcgccatga agacgatcat cgccctgagc
      301 tacatcttct gcctggtgtt cgccgactac aaggacgatg atgacgccat gaggactctg
      361 aacacctctg ccatggacgg gactgggctg gtggtggaga gggacttctc tgttcgtatc
      421 ctcactgcct gtttcctgtc gctgctcatc ctgtccacgc tcctggggaa cacgctggtc
      481 tgtgctgccg ttatcaggtt ccgacacctg cggtccaagg tgaccaactt ctttgtcatc
      541 tccttggctg tgtcagatct cttggtggcc gtcctggtca tgccctggaa ggcagtggct
      601 gagattgctg gcttctggcc ctttgggtcc ttctgtaaca tctgggtggc ctttgacatc
      661 atgtgctcca ctgcatccat cctcaacctc tgtgtgatca gcgtggacag gtattgggct
      721 atctccagcc ctttccggta tgagagaaag atgaccccca aggcagcctt catcctgatc
      781 agtgtggcat ggaccttgtc tgtactcatc tccttcatcc cagtgcagct cagctggcac
      841 aaggcaaaac ccacaagccc ctctgatgga aatgccactt ccctggctga gaccatagac
      901 aactgtgact ccagcctcag caggacatat gccatctcat cctctgtaat cagcttttac
      961 atccctgtgg ccatcatgat tgtcacctac accaggatct acaggattgc tcagaaacag
     1021 ctgagctcac tcattaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg
     1081 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag
     1141 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg
     1201 cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1261 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg
     1321 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1381 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc
     1441 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1501 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     1561 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc
     1621 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1681 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1741 cacaagctgg agtacaacaa tcatgaccaa ctgaaaagag aaactaaagt cctgaagact
     1801 ctgtcggtga tcatgggtgt gtttgtgtgc tgttggctac ctttcttcat cttgaactgc
     1861 attttgccct tctgtgggtc tggggagacg cagcccttct gcattgattc caacaccttt
     1921 gacgtgtttg tgtggtttgg gtgggctaat tcatccttga accccatcat ttatgccttt
     1981 aatgctgatt ttcggaaggc attttcaacc ctcttaggat gctacagact ttgccctgcg
     2041 acgaataatg ccatagagac ggtgagtatc aataacaatg gggccgcgat gttttccagc
     2101 catcatgagc cacgaggctc catctccaag gagtgcaatc tggtttacct gatcccacat
     2161 gctgtgggct cctctgagga cctgaaaaag gaggaggcag ctggcatcgc cagacccttg
     2221 gagaagctgt ccccagccct atcggtcata ttggactatg acactgacgt ctctctggag
     2281 aagatccaac ccatcacaca aaacggtcag cacccaacct gatgtacagc ggccgcgact
     2341 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     2401 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2461 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2521 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     2581 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     2641 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     2701 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     2761 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     2821 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     2881 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     2941 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     3001 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     3061 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3121 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     3181 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     3241 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     3301 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     3361 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3421 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3481 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     3541 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     3601 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3661 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3721 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3781 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3841 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3901 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3961 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4021 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4081 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4141 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4201 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4261 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     4321 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     4381 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     4441 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     4501 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     4561 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     4621 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     4681 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     4741 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     4801 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4861 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4921 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4981 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5041 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5101 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5161 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5221 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5281 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5341 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5401 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5461 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5521 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5581 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5641 taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc
     5701 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     5761 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     5821 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     5881 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     5941 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     6001 acgtattagt catcgctatt accatg
//
