LOCUS       Exported                7063 bp ds-DNA     circular SYN 20-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-AVV-KPT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 20, 2018 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     LOCUS Exported 6373 bp ds-DNA circular SYN 16-JUN-2015 DEFINITION 
            synthetic circular DNA ACCESSION . VERSION . KEYWORDS AAV-pCAG-G3 
            SOURCE synthetic DNA construct ORGANISM synthetic DNA construct 
            REFERENCE 1 (bases 1 to 6373) AUTHORS . TITLE Direct Submission 
            JOURNAL Exported Jun 16, 2015 from SnapGene Viewer 2.6.0 
            http://www.snapgene.com COMMENT Created: Thursday, September 06, 
            2007 6:30 PM
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1394..1401
                     /note="source [5']"
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..1351"
     source          1402..2142
                     /note="source [*]"
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..2185"
     source          2143..2151
                     /note="source [5']"
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..1351"
     source          2896..3444
                     /note="source [*]"
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..2185"
     misc_feature    259..426
                     /note="ITR"
     promoter        451..1385
                     /note="CBA promoter"
     CDS             1402..3444
                     /codon_start=1
                     /note="dLight1.1"
                     /translation="MKTIIALSYIFCLVFADYKDDDDAMRTLNTSAMDGTGLVVERDFS
                     VRILTACFLSLLILSTLLGNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPW
                     KAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVDRYWAISSPFRYERKMTPKA
                     AFILISVAWTLSVLISFIPVQLSWHKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISS
                     SVISFYIPVAIMIVTYTRIYRIAQKLSSLINVYIKADKQKNGIKANFKIRHNIEDGGVQ
                     LAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDEL
                     YKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTT
                     GKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTR
                     AEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNNHDQLKRETKVLKTLSVIMGVFVC
                     CWLPFFILNCILPFCGSGETQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAFS
                     TLLGCYRLCPATNNAIETVSINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDL
                     KKEEAAGIARPLEKLSPALSVILDYDTDVSLEKIQPITQNGQHPT"
     misc_feature    1402..1449
                     /note="HA secretory sequence_misc_feature"
                     /note="note=""HA secretory sequence"" - 23..70"
     CDS             1450..1473
                     /note="FLAG_CDS"
                     /note="codon_start=1 - note=""FLAG"" - 
                     translation=""DYKDDDDA"" - 71..94"
     CDS             1474..2142
                     /note="DRD1_CDS [*]"
                     /note="codon_start=1 - note=""DRD1"" - 
                     translation=""MRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVC
                      - 
                     AAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDI
                      - 
                     MCSTASILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSW
                      - HKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISSSVISF"
     misc_feature    2050..2127
                     /note="TM5_misc_feature"
                     /note="note=""TM5"" - 671..748"
     CDS             2896..3444
                     /note="DRD1_CDS [*]"
                     /note="codon_start=1 - note=""DRD1"" - 
                     translation=""MRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVC
                      - 
                     AAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDI
                      - 
                     MCSTASILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSW
                      - HKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISSSVISF"
     CDS             3112..3138
                     /note="hydrophobic ER export motif_CDS"
                     /note="codon_start=1 - note=""hydrophobic ER export motif""
                     - translation=""FRKAFSTLL"" - 1838..1864"
     misc_feature    3457..4045
                     /note="WPRE"
     misc_feature    4073..4305
                     /note="SV40"
     misc_feature    4333..4500
                     /note="ITR"
     CDS             5263..6123
                     /codon_start=1
                     /note="AMP"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
      721 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      781 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
      841 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      901 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
      961 aagcgcgcgg cgggcgggag tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg
     1021 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     1081 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
     1141 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
     1201 gctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     1261 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     1321 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     1381 gaattggatc cggtgcgcgc catgaagacg atcatcgccc tgagctacat cttctgcctg
     1441 gtgttcgccg actacaagga cgatgatgac gccatgagga ctctgaacac ctctgccatg
     1501 gacgggactg ggctggtggt ggagagggac ttctctgttc gtatcctcac tgcctgtttc
     1561 ctgtcgctgc tcatcctgtc cacgctcctg gggaacacgc tggtctgtgc tgccgttatc
     1621 aggttccgac acctgcggtc caaggtgacc aacttctttg tcatctcctt ggctgtgtca
     1681 gatctcttgg tggccgtcct ggtcatgccc tggaaggcag tggctgagat tgctggcttc
     1741 tggccctttg ggtccttctg taacatctgg gtggcctttg acatcatgtg ctccactgca
     1801 tccatcctca acctctgtgt gatcagcgtg gacaggtatt gggctatctc cagccctttc
     1861 cggtatgaga gaaagatgac ccccaaggca gccttcatcc tgatcagtgt ggcatggacc
     1921 ttgtctgtac tcatctcctt catcccagtg cagctcagct ggcacaaggc aaaacccaca
     1981 agcccctctg atggaaatgc cacttccctg gctgagacca tagacaactg tgactccagc
     2041 ctcagcagga catatgccat ctcatcctct gtaatcagct tttacatccc tgtggccatc
     2101 atgattgtca cctacaccag gatctacagg attgctcaga aactgagctc actcattaac
     2161 gtctatatca aggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac
     2221 aacatcgagg acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc
     2281 gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccaa actttcgaaa
     2341 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2401 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc
     2461 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2521 cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa gctgaccctg
     2581 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     2641 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     2701 aagtccgcca tgcccgaagg ctacatccag gagcgcacca tcttcttcaa ggacgacggc
     2761 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     2821 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2881 aatcatgacc aactgaaaag agaaactaaa gtcctgaaga ctctgtcggt gatcatgggt
     2941 gtgtttgtgt gctgttggct acctttcttc atcttgaact gcattttgcc cttctgtggg
     3001 tctggggaga cgcagccctt ctgcattgat tccaacacct ttgacgtgtt tgtgtggttt
     3061 gggtgggcta attcatcctt gaaccccatc atttatgcct ttaatgctga ttttcggaag
     3121 gcattttcaa ccctcttagg atgctacaga ctttgccctg cgacgaataa tgccatagag
     3181 acggtgagta tcaataacaa tggggccgcg atgttttcca gccatcatga gccacgaggc
     3241 tccatctcca aggagtgcaa tctggtttac ctgatcccac atgctgtggg ctcctctgag
     3301 gacctgaaaa aggaggaggc agctggcatc gccagaccct tggagaagct gtccccagcc
     3361 ctatcggtca tattggacta tgacactgac gtctctctgg agaagatcca acccatcaca
     3421 caaaacggtc agcacccaac ctgaaagctt atcgataatc aacctctgga ttacaaaatt
     3481 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3541 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3601 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3661 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3721 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3781 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3841 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     3901 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3961 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4021 atctcccttt gggccgcctc cccgcatcga taccgtcgac ccgggcggcc gcttcgagca
     4081 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4141 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4201 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
     4261 gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct
     4321 agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct
     4381 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     4441 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     4501 ccttaattaa cctaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4561 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     4621 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     4681 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4741 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4801 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4861 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4921 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4981 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5041 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5101 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     5161 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5221 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5281 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5341 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5401 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5461 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5521 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5581 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5641 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5701 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5761 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5821 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5881 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5941 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6001 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6061 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6121 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6181 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6241 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6301 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6361 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6421 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6481 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6541 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6601 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6661 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6721 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6781 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6841 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6901 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6961 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7021 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//
