LOCUS       Exported                6622 bp ds-DNA     circular SYN 20-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    AAV-Syn-AVKP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 20, 2018 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     Ligation of : AAV-Syn-GCaMP6.gb.xdna [4559 nt] : (HindIII[2308] / 
            BamHI[939]) to Sequence Window #2 [2073 nt] : (BamHI[1] / 
            HindIII[2078])
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          947..954
                     /note="source [5']"
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..1351"
     source          955..1695
                     /note="source [*]"
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..2185"
     source          1696..1704
                     /note="source [5']"
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..1351"
     source          2449..2997
                     /note="source [*]"
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..2185"
     misc_feature    260..426
                     /note="ITR_misc_feature"
                     /note="note=""ITR"" - 260..426"
     misc_feature    443..448
                     /note="BglII_misc_feature"
                     /note="note=""BglII"" - 443..448"
     misc_feature    449..920
                     /note="synapsin promoter_misc_feature"
                     /note="note=""synapsin promoter"" - 449..920"
     CDS             955..2997
                     /codon_start=1
                     /note="dLight1.1"
                     /translation="MKTIIALSYIFCLVFADYKDDDDAMRTLNTSAMDGTGLVVERDFS
                     VRILTACFLSLLILSTLLGNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPW
                     KAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVDRYWAISSPARYERKMTPKA
                     AFILISVAWTLSVLISFIPVQLSWHKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISS
                     SVISFYIPVAIMIVTYTRIYRIAQKLSSLINVYIKADKQKNGIKANFKIRHNIEDGGVQ
                     LAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDEL
                     YKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTT
                     GKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTR
                     AEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNNHDQLKRETKVLKTLSVIMGVFVC
                     CWLPFFILNCILPFCGSGETQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAFS
                     TLLGCYRLCPATNNAIETVSINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDL
                     KKEEAAGIARPLEKLSPALSVILDYDTDVSLEKIQPITQNGQHPT"
     misc_feature    955..1002
                     /note="HA secretory sequence_misc_feature"
                     /note="note=""HA secretory sequence"" - 23..70"
     CDS             1003..1026
                     /note="FLAG_CDS"
                     /note="codon_start=1 - note=""FLAG"" - 
                     translation=""DYKDDDDA"" - 71..94"
     CDS             1027..1695
                     /note="DRD1_CDS [*]"
                     /note="codon_start=1 - note=""DRD1"" - 
                     translation=""MRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVC
                      - 
                     AAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDI
                      - 
                     MCSTASILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSW
                      - HKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISSSVISF"
     misc_feature    1603..1680
                     /note="TM5_misc_feature"
                     /note="note=""TM5"" - 671..748"
     CDS             2449..2997
                     /note="DRD1_CDS [*]"
                     /note="codon_start=1 - note=""DRD1"" - 
                     translation=""MRTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVC
                      - 
                     AAVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDI
                      - 
                     MCSTASILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSW
                      - HKAKPTSPSDGNATSLAETIDNCDSSLSRTYAISSSVISF"
     CDS             2665..2691
                     /note="hydrophobic ER export motif_CDS"
                     /note="codon_start=1 - note=""hydrophobic ER export motif""
                     - translation=""FRKAFSTLL"" - 1838..1864"
     misc_feature    3010..3598
                     /note="WPRE_misc_feature"
                     /note="note=""WPRE"" - 2320..2908"
     misc_feature    3146..3166
                     /note="Mao 115r_misc_feature"
                     /note="note=""Mao 115r"" - 2456..2476"
     misc_feature    3632..3863
                     /note="SV40_misc_feature"
                     /note="note=""SV40"" - 2942..3173"
     misc_feature    3892..4058
                     /note="ITR_misc_feature"
                     /note="note=""ITR"" - 3202..3368"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatctct gcagagggcc ctgcgtatga gtgcaagtgg
      481 gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac cccgacccac
      541 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      601 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      661 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      721 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      781 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      841 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
      901 cgtgcctgag agcgcagtcg aattcaagct gctagcaagg atccggtgcg cgccatgaag
      961 acgatcatcg ccctgagcta catcttctgc ctggtgttcg ccgactacaa ggacgatgat
     1021 gacgccatga ggactctgaa cacctctgcc atggacggga ctgggctggt ggtggagagg
     1081 gacttctctg ttcgtatcct cactgcctgt ttcctgtcgc tgctcatcct gtccacgctc
     1141 ctggggaaca cgctggtctg tgctgccgtt atcaggttcc gacacctgcg gtccaaggtg
     1201 accaacttct ttgtcatctc cttggctgtg tcagatctct tggtggccgt cctggtcatg
     1261 ccctggaagg cagtggctga gattgctggc ttctggccct ttgggtcctt ctgtaacatc
     1321 tgggtggcct ttgacatcat gtgctccact gcatccatcc tcaacctctg tgtgatcagc
     1381 gtggacaggt attgggctat ctccagccct gcccggtatg agagaaagat gacccccaag
     1441 gcagccttca tcctgatcag tgtggcatgg accttgtctg tactcatctc cttcatccca
     1501 gtgcagctca gctggcacaa ggcaaaaccc acaagcccct ctgatggaaa tgccacttcc
     1561 ctggctgaga ccatagacaa ctgtgactcc agcctcagca ggacatatgc catctcatcc
     1621 tctgtaatca gcttttacat ccctgtggcc atcatgattg tcacctacac caggatctac
     1681 aggattgctc agaaactgag ctcactcatt aacgtctata tcaaggccga caagcagaag
     1741 aacggcatca aggcgaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc
     1801 gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1861 cactacctga gcgtgcagtc caaactttcg aaagacccca acgagaagcg cgatcacatg
     1921 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1981 ggcggtaccg gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2041 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt
     2101 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2161 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     2221 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc
     2281 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2341 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2401 ggcaacatcc tggggcacaa gctggagtac aacaatcatg accaactgaa aagagaaact
     2461 aaagtcctga agactctgtc ggtgatcatg ggtgtgtttg tgtgctgttg gctacctttc
     2521 ttcatcttga actgcatttt gcccttctgt gggtctgggg agacgcagcc cttctgcatt
     2581 gattccaaca cctttgacgt gtttgtgtgg tttgggtggg ctaattcatc cttgaacccc
     2641 atcatttatg cctttaatgc tgattttcgg aaggcatttt caaccctctt aggatgctac
     2701 agactttgcc ctgcgacgaa taatgccata gagacggtga gtatcaataa caatggggcc
     2761 gcgatgtttt ccagccatca tgagccacga ggctccatct ccaaggagtg caatctggtt
     2821 tacctgatcc cacatgctgt gggctcctct gaggacctga aaaaggagga ggcagctggc
     2881 atcgccagac ccttggagaa gctgtcccca gccctatcgg tcatattgga ctatgacact
     2941 gacgtctctc tggagaagat ccaacccatc acacaaaacg gtcagcaccc aacctgaaag
     3001 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3061 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3121 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3181 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3241 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3301 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3361 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3421 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3481 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3541 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     3601 cgataccgtc gacctcgacc cgggcggccg cttcgagcag acatgataag atacattgat
     3661 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     3721 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     3781 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
     3841 aacctctaca aatgtggtaa aatcgataag gatcttccta gagcatggct acgtagataa
     3901 gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc
     3961 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4021 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc cttaattaac ctaattcact
     4081 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     4141 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4201 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     4261 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4321 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4381 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4441 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4501 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4561 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4621 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4681 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4741 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4801 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4861 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4921 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4981 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5041 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5101 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5161 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5221 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5281 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5341 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5401 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5461 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5521 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5581 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5641 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5701 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5761 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5821 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5881 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5941 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6001 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6061 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6121 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6181 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6241 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6301 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6361 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6421 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6481 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6541 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6601 tcagtgagcg aggaagcgga ag
//
