LOCUS       Exported               10730 bp ds-DNA     circular SYN 20-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 20, 2018 from SnapGene Viewer 4.1.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             329..988
                     /label=AmpR
     rep_origin      1086..1768
                     /label=ColE1 origin
     promoter        2074..2104
                     /label=lac promoter
     misc_binding    2108..2130
                     /label=LacO
     primer_bind     2136..2156
                     /label=M13-rev
     promoter        2173..2191
                     /label=T3 promoter
     primer_bind     2174..2193
                     /label=T3
     misc_recomb     4171..4191
                     /label=attB1
     misc_feature    4171..4191
                     /label=Gateway recomb1
     misc_feature    4171..4177
                     /label=ATTR1 site
     misc_feature    4178..4188
                     /label=ATTL1 site
     misc_feature    4325..5504
                     /label=hEF1a Promoter
     primer_bind     5521..5540
                     /label=T7
                     /label=T7(1)
     misc_feature    5578..5583
                     /label=BamHI site
     misc_feature    5584..5613
                     /label=HA tag
     misc_feature    5614..5637
                     /label=LINKER SEQ
     misc_feature    6232..6237
                     /label=SALI
     misc_signal     6238..6812
                     /label=IRES
     CDS             6813..7307
                     /label=Thy1.1
     misc_feature    7305..7307
                     /label=STOP
     misc_feature    7308..7313
                     /label=XbaI
     misc_recomb     complement(7316..7336)
                     /label=attB2
     misc_feature    complement(7316..7336)
                     /label=Gateway Recomb2
     misc_feature    7319..7322
                     /label=ATTL2 site
     misc_feature    7323..7336
                     /label=ATTR2 site
     misc_feature    7363..7959
                     /label=WPRE
     promoter        8007..8506
                     /label=PGK promoter
     CDS             8532..9323
                     /label=Kan/neoR
     primer_bind     complement(10088..10108)
                     /label=T7
     primer_bind     complement(10117..10134)
                     /label=M13-fwd
     CDS             10205..10273
                     /label=LacZ alpha
     rep_origin      10292..10598
                     /label=F1 ori
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctccacca
     4201 tgggaaccga cattgattat tgactaggct tttgcaaaaa gctttgcaaa gatggataaa
     4261 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     4321 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     4381 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     4441 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     4501 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     4561 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     4621 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     4681 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     4741 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     4801 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     4861 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     4921 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca gtgttcggcg aggcggggcc
     4981 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     5041 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     5101 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     5161 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     5221 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     5281 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     5341 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     5401 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     5461 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt agcttggtac
     5521 taatacgact cactataggg agacccaagc tggctaggta agcttggtac cgagctcgga
     5581 tccatgtacc catacgatgt tccagattac gctggtggat ctggaggttc tggtggaatg
     5641 cagagagctt cacgtctgaa gagagagctg cacatgttag ccacagagcc acccccaggc
     5701 atcacatgtt ggcaagataa agaccaaatg gatgacctgc gagctcaaat attaggtgga
     5761 gccaacacac cttatgagaa aggtgttttt aagctagaag ttatcattcc tgagaggtac
     5821 ccatttgaac ctcctcagat ccgatttctc actccaattt atcatccaaa cattgattct
     5881 gctggaagga tttgtctgga tgttctcaaa ttgccaccaa aaggtgcttg gagaccatcc
     5941 ctcaacatcg caactgtgtt gacctctatt cagctgctca tgtcagaacc caaccctgat
     6001 gacccgctca tggctgacat atcctcagaa tttaaatata ataagccagc cttcctcaag
     6061 aatgccagac agtggacaga gaagcatgca agacagaaac aaaaggctga tgaggaagag
     6121 atgcttgata atctaccaga ggctggtgac tccagagtac acaactcaac acagaaaagg
     6181 aaggccagtc agctagtagg catagaaaag aaatttcatc ctgatgttta ggtcgacccc
     6241 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     6301 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     6361 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     6421 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     6481 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     6541 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     6601 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     6661 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     6721 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     6781 cgtggttttc ctttgaaaaa cacgatgcca ccatgggtat gaacccagcc atcagcgtcg
     6841 ctctcctgct ctcagtcttg caggtgtccc gagggcagaa ggtgaccagc ctgacagcct
     6901 gcctggtgaa ccaaaacctt cgcctggact gccgccatga gaataacacc aaggataact
     6961 ccatccagca tgagttcagc ctgacccgag agaagaggaa gcacgtgctc tcaggcaccc
     7021 ttgggatacc cgagcacacg taccgctccc gcgtcaccct ctccaaccag ccctatatca
     7081 aggtccttac cctagccaac ttcaccacca aggatgaggg cgactacttt tgtgagcttc
     7141 gcgtctcggg cgcgaatccc atgagctcca ataaaagtat cagtgtgtat agagacaagc
     7201 tggtcaagtg tggcggcata agcctgctgg ttcagaacac atcctggatg ctgctgctgc
     7261 tgctttccct ctccctcctc caagccctgg acttcatttc tctgtgatct agacccagct
     7321 ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgacaat caacctctgg
     7381 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     7441 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     7501 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     7561 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     7621 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     7681 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     7741 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     7801 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     7861 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     7921 agacgagtcg gatctccctt tgggccgcct ccccgcctgg acctgcagga ttctgcagat
     7981 atccggacta gtgatctaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     8041 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     8101 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     8161 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     8221 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     8281 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     8341 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     8401 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     8461 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagccaata
     8521 tgggatcggc cattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     8581 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     8641 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     8701 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     8761 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     8821 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     8881 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     8941 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     9001 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     9061 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     9121 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     9181 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     9241 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     9301 gccttcttga cgagttcttc tgaacgcgtc gcccgcccca cgacccgcag cgcccgaccg
     9361 aaaggagcgc acgaccccat gcatctcgag ggcccggtac ctttaagacc aatgacttac
     9421 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctagct
     9481 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     9541 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     9601 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9661 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     9721 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     9781 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9841 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9901 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     9961 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    10021 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
    10081 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
    10141 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
    10201 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
    10261 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
    10321 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
    10381 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
    10441 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
    10501 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
    10561 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
    10621 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
    10681 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
//
