LOCUS       pCAG-mTrex2-bpA-EF1BFP      7692 bp    DNA     circular UNA 12-DEC-2009
DEFINITION  
ACCESSION   pCAG-cre-bpA
DBSOURCE    GenBank
FEATURES             Location/Qualifiers
     misc_feature    7..22
                     /label="M13 rev"
                     /label=""M13 rev""
     promoter        38..1708
                     /note="Oriinal location: 87..1753"
                     /label="CAG Promoter"
                     /label=""CAG Promoter""
     promoter        1716..1738
                     /label="T7 Promoter"
     CDS             1748..2458
                     /label="Trex2 coding region (mouse)"
     polyA_signal    2493..2764
                     /note="Oriinal location: 2900..3171"
                     /note="pA from bovine growth hormone"
                     /label=bpA
     promoter        2835..3934
                     /label="EF1a promoter"
     CDS             3966..4685
                     /label=BFP
     polyA_signal    4686..4917
                     /label=bpA
     misc_feature    complement(4945..4961)
                     /label="M13 for"
                     /label=""M13 for""
     CDS             5689..6549
                     /label="amp resistance"
ORIGIN
        1 ACAGGAAACA GCTATGACCA TGAGGCGCGC CGGATTCGAC ATTGATTATT GACTAGTTAT
       61 TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA
      121 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA
      181 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG
      241 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG
      301 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC
      361 TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTC
      421 GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC CCCCCTCCCC ACCCCCAATT
      481 TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG GGGCGGGGGG GGGGGGGGGG
      541 CGCGCGCCAG GCGGGGCGGG GCGGGGCGAG GGGCGGGGCG GGGCGAGGCG GAGAGGTGCG
      601 GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA AAGTTTCCTT TTATGGCGAG GCGGCGGCGG
      661 CGGCGGCCCT ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGTC GCGCCTTCGC
      721 CCCGTGCCCC GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT GACCGCGTTA
      781 CTCCCACAGG TGAGCGGGCG GGACGGCCCT TCTCCTCCGG GCTGTAATTA GCGCTTGGTT
      841 TAATGACGGC TCGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTAAAGGGCT CCGGGAGGGC
      901 CCTTTGTGCG GGGGGGAGCG GCTCGGGGGG TGCGTGCGTG TGTGTGTGCG TGGGGAGCGC
      961 CGCGTGCGGC CCGCGCTGCC CGGCGGCTGT GAGCGCTGCG GGCGCGGCGC GGGGCTTTGT
     1021 GCGCTCCGCG TGTGCGCGAG GGGAGCGCGG CCGGGGGCGG TGCCCCGCGG TGCGGGGGGG
     1081 CTGCGAGGGG AACAAAGGCT GCGTGCGGGG TGTGTGCGTG GGGGGGTGAG CAGGGGGTGT
     1141 GGGCGCGGCG GTCGGGCTGT AACCCCCCCC TGCACCCCCC TCCCCGAGTT GCTGAGCACG
     1201 GCCCGGCTTC GGGTGCGGGG CTCCGTGCGG GGCGTGGCGC GGGGCTCGCC GTGCCGGGCG
     1261 GGGGGTGGCG GCAGGTGGGG GTGCCGGGCG GGGCGGGGCC GCCTCGGGCC GGGGAGGGCT
     1321 CGGGGGAGGG GCGCGGCGGC CCCGGAGCGC CGGCGGCTGT CGAGGCGCGG CGAGCCGCAG
     1381 CCATTGCCTT TTATGGTAAT CGTGCGAGAG GGCGCAGGGA CTTCCTTTGT CCCAAATCTG
     1441 GCGGAGCCGA AATCTGGGAG GCGCCGCCGC ACCCCCTCTA GCGGGCGCGG GCGAAGCGGT
     1501 GCGGCGCCGG CAGGAAGGAA ATGGGCGGGG AGGGCCTTCG TGCGTCGCCG CGCCGCCGTC
     1561 CCCTTCTCCA TCTCCAGCCT CGGGGCTGCC GCAGGGGGAC GGCTGCCTTC GGGGGGGACG
     1621 GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT GACCGGCGGC TCTAGAGCCT CTGCTAACCA
     1681 TGTTCATGCC TTCTTCTTTT TCCTACAGAT CCTTATAATA ATACGACTCA CTATAGGGgc
     1741 cgccaccATG TCTGAGCCAC CCCGGGCTGA GACCTTTGTA TTCCTGGACC TAGAAGCCAC
     1801 TGGGCTCCCA AACATGGATC CTGAGATTGC AGAGATATCC CTTTTTGCTG TTCACCGCTC
     1861 TTCCCTGGAG AACCCAGAAC GGGATGATTC TGGTTCCTTG GTGCTGCCCC GTGTTCTGGA
     1921 CAAGCTCACA CTGTGCATGT GCCCGGAGCG CCCCTTTACT GCCAAGGCCA GTGAGATTAC
     1981 TGGTTTGAGC AGCGAAAGCC TGATGCACTG CGGGAAGGCT GGTTTCAATG GCGCTGTGGT
     2041 AAGGACACTG CAGGGCTTCC TAAGCCGCCA GGAGGGCCCC ATCTGCCTTG TGGCCCACAA
     2101 TGGCTTCGAT TATGACTTCC CACTGCTGTG CACGGAGCTA CAACGTCTGG GTGCCCATCT
     2161 GCCCCAAGAC ACTGTCTGCC TGGACACACT GCCTGCATTG CGGGGCCTGG ACCGTGCTCA
     2221 CAGCCACGGC ACCAGGGCTC AAGGCCGCAA AAGCTACAGC CTGGCCAGTC TCTTCCACCG
     2281 CTACTTCCAG GCTGAACCCA GTGCTGCCCA TTCAGCAGAA GGTGATGTGC ACACCCTGCT
     2341 TCTGATCTTC CTGCATCGTG CTCCTGAGCT GCTCGCCTGG GCAGATGAGC AGGCCCGCAG
     2401 CTGGGCTCAT ATTGAGCCCA TGTACGTGCC ACCTGATGGT CCAAGCCTCG AAGCCTGAAC
     2461 GCGTAAATGA TTGCAGATCC ACTAGTTCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT
     2521 CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG
     2581 CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT
     2641 GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA
     2701 ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT
     2761 GGGGCTCGAT AATAATACGA CTCACTATAG GGGCCGCCAC CATGGGACCT AAGAAAAAGA
     2821 GGAAGGTGGC ggccCCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT
     2881 CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT
     2941 CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGGTAA GTGCCGTGTG
     3001 TGGTTCCCGC GGGCCTGGCC TCTTTACGGG TTATGGCCCT TGCGTGCCTT GAATTACTTC
     3061 CACCTGGCTG CAGTACGTGA TTCTTGATCC CGAGCTTCGG GTTGGAAGTG GGTGGGAGAG
     3121 TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG CCTGGCCTGG
     3181 GCGCTGGGGC CGCCGCGTGC GAATCTGGTG GCACCTTCGC GCCTGTCTCG CTGCTTTCGA
     3241 TAAGTCTCTA GCCATTTAAA ATTTTTGATG ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA
     3301 TAGTCTTGTA AATGCGGGCC AAGATCTGCA CACTGGTATT TCGGTTTTTG GGGCCGCGGG
     3361 CGGCGACGGG GCCCGTGCGT CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC TGCGAGCGCG
     3421 GCCACCGAGA ATCGGACGGG GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG TGCCTGGCCT
     3481 CGCGCCGCCG TGTATCGCCC CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG CACCAGTTGC
     3541 GTGAGCGGAA AGATGGCCGC TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT GGAGGACGCG
     3601 GCGCTCGGGA GAGCGGGCGG GTGAGTCACC CACACAAAGG AAAAGGGCCT TTCCGTCCTC
     3661 AGCCGTCGCT TCATGTGACT CCACGGAGTA CCGGGCGCCG TCCAGGCACC TCGATTAGTT
     3721 CTCGAGCTTT TGGAGTACGT CGTCTTTAGG TTGGGGGGAG GGGTTTTATG CGATGGAGTT
     3781 TCCCCACACT GAGTGGGTGG AGACTGAAGT TAGGCCAGCT TGGCACTTGA TGTAATTCTC
     3841 CTTGGAATTT GCCCTTTTTG AGTTTGGATC TTGGTTCATT CTCAAGCCTC AGACAGTGGT
     3901 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAgcgatc gcatcgacag tGTTTAAACg
     3961 ccaccATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC
     4021 TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGAGGGG CGAGGGCGAG GGCGATGCCA
     4081 CCAACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC
     4141 CCACCCTCGT GACCACCCTG AGCCACGGCG TGCAGTGCTT CGCCCGCTAC CCCGACCACA
     4201 TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA
     4261 TCTTCTTCAA GGACGACGGC ACCTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA
     4321 CCCTGGTGAA CCGCATCGAG CTGAAGGGCG TCGACTTCAA GGAGGACGGC AACATCCTGG
     4381 GGCACAAGCT GGAGTACAAC TTCAACAGCC ACAACATCTA TATCATGGCC GTCAAGCAGA
     4441 AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACGT GGAGGACGGC AGCGTGCAGC
     4501 TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA
     4561 GCCACTACCT GAGCACCCAG TCCGTGCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA
     4621 TGGTCCTGCT GGAGTTCCGC ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA
     4681 AGTGAGAATT CCTAGAGCTC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC
     4741 TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT
     4801 TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG
     4861 GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GAGAATAGCA GGCATGCgtt
     4921 taaacattaG TCGACAGGGC CGCCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA
     4981 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA
     5041 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG
     5101 GGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC
     5161 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC
     5221 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT
     5281 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG
     5341 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG
     5401 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT
     5461 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT
     5521 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG GTGGCACTTT TCGGGGAAAT
     5581 GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG
     5641 AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA
     5701 CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC
     5761 CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC
     5821 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT
     5881 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC
     5941 GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA
     6001 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC
     6061 ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG
     6121 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA
     6181 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG
     6241 GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA
     6301 TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG
     6361 GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT
     6421 GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT
     6481 CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG
     6541 CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT
     6601 TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT
     6661 TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT
     6721 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA
     6781 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC
     6841 AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC
     6901 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT
     6961 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG
     7021 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC
     7081 TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG
     7141 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG
     7201 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT
     7261 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC
     7321 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG
     7381 TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC
     7441 CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA
     7501 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT
     7561 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG
     7621 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA
     7681 TAACAATTTC AC
//
