LOCUS       Exported               10029 bp ds-DNA     circular SYN 18-APR-2018
DEFINITION  Col2A1-TFpnls-T2a-ERt-Cre-ERt.
ACCESSION   Col2A1-TFpnls-T2a-ERt-Cre-ERt
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Self
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 10029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 26, 2018 from SnapGene Viewer 4.1.8
            http://www.snapgene.com
COMMENT     Textco/File created by Gene Construction Kit (Textco BioSoftware)
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..453
                     /label=F1 Ori
     misc_feature    585..607
                     /label=M13_Fw23
     misc_feature    624..641
                     /label=Sce I site
     misc_feature    648..669
                     /label=T7 Promoter
     misc_feature    682..889
                     /label=miniTol2Left
     misc_feature    890..2901
     misc_feature    890..2901
                     /label=PM-45-Col2A1 copy
     misc_feature    890..1384
                     /label=Promoter
     misc_feature    1385..2901
                     /label=Xenopus Col2a1-First Intron Enhancer
     misc_feature    2902..2907
                     /label=PM-27
     misc_feature    2944..3151
     misc_feature    2944..3151
                     /label=PM-02-MCS-D-USER 2
     misc_feature    2944..3151
                     /label=PM-02-MCS-D-USER 2
     misc_feature    2944..3151
                     /label=b-Globin Intron
     misc_feature    3263..3279
     misc_feature    3287..3994
                     /label=TFP
     misc_feature    3986..4018
                     /label=PR-639
     misc_feature    3995..4100
                     /label=NLS
     misc_feature    4109..4162
                     /label=T2A
     CDS             4169..7090
                     /label=ERt-Cre-ERt
     misc_feature    complement(5264..5287)
                     /label=PR-643
     misc_feature    7100..7623
                     /label=Poly A
     misc_feature    7642..7791
                     /label=mTol2Right
     misc_feature    complement(7817..7836)
                     /label=T3 Promoter
     misc_feature    complement(7843..7860)
                     /label=Sce I Site
     misc_feature    complement(7893..7915)
                     /label=M13_Rv23
     misc_feature    complement(8264..8946)
                     /label=ColE1 origin
     misc_feature    complement(9041..9901)
                     /label=Amp Res
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgtagggat aacagggtaa tgcgcgcgta atacgactca
      661 ctatagggcg aattgggtac ccagaggtgt aaagtacttg agtaatttta cttgattact
      721 gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac
      781 ttttactttt acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt
      841 ttatttacag tcaaaaagta cttatttttt ggagatcact tgggcccggc tttcctgcaa
      901 gctcactgta gaaaagagac ctgctttaac aaattgcagt ttttttcctg cctctctcta
      961 aatggtagca cttttttttt tgacagagga atgagagagt ttaggtgagg ggggcgaagg
     1021 agggaagaga gagagcagga gggtttggga cagtgggagg gaggcagaag gggacagaaa
     1081 gtggcaagca agggaaaggg tccctgcaag aaaggagggg tttggtggga tggggagagg
     1141 gcagagagga gtggaggggc tcagatttca gctctgctgg gggcagggga ggactaagat
     1201 gtgggcatat acgttgactt caacccacac aggagtataa cttcagtacc ttggcccaga
     1261 gcagttagta ctaagtttct gctggggcct ggtactggat tctccttctt ttcccaagaa
     1321 atcggctgca tgaagacaag ataactattg gagacagcca tcctccttct ccccttctga
     1381 gctcgagaaa aatgtgcaac ttgctggggc aggtcccaat ggttagagaa aaaaataaaa
     1441 attcctttac gagtccttca catactacag actgtaaatc cattgcactg ctagtatgga
     1501 aaaggcatct ttttctgaat atttgccagg tttttttttt attggaatac ctattctttt
     1561 ccataaaacg ttcttaatct gaactgcaca tctgtagaaa ggggaatatg tgtgaaatgc
     1621 tcaagtagca ggctggaatc tctctcagct gatctactgt ctgtatagtt tccagtcaaa
     1681 gattgctttt catagcttaa atcagggctt tcagagggtt ctgggagttg tagttttaag
     1741 atagctgaaa agttacagcc ccctgcctta aatcatatac ttgctaaata catagtttac
     1801 cattaatacg attactggaa aatagacatg cttacctatg gcctttgaac tgttgttgaa
     1861 ctacaatccc caccagtcct tggctgtcga ggaatgctgg gagttgtagt ttattagcta
     1921 gaggacagaa ggttagacat ctctgatgca gaaaatagat tttgggtggc acctgtcaac
     1981 ttgagggttt gcatgttata taattccccc ttgaggagaa agcaataata aaggaaaaca
     2041 tactcatatg ggaattctat aaactcatag aggaaacctt ataagtatgt tattgttgaa
     2101 gactgctctg ggatatgagg aaatgctcaa aactttttaa tgtacgcata ttaagtctag
     2161 aggtccatcc gctgtactta taaaggtgat taaaatggaa gggccttgct taaaggcttc
     2221 aattcagtat tgtgatggtt tatttgcact gggaggattg gcagcaatgg tttccagatg
     2281 gagttgaaat gctgcccatg tttatttaaa ctggcccctg acagtgaaat gtgaattgag
     2341 acctctgtgt attttacaaa aaggcccatt cattaagggt cctctgggct acccaagcat
     2401 gggccatgga cccaacggga gtgatgcagg gtctaatcaa gatcactgct tgggtcacaa
     2461 tggattaata tgacatcctt tgttatctaa tagcctttat taacagaatc tcatcctacc
     2521 tttcaacaaa tgtgagaact agttggtatg taccattctt cccagtgttt gatcttagca
     2581 aacattcttg ataagtacag atttttcatc cagtagctgc attttattca attatgctcc
     2641 tttttagcct ttactatttc ctattaattt gctttatgag aaacagtgaa gacaccttag
     2701 ggagtctaat ttcaaaagca gattctctag cttttaatga cctcagggag actgttatta
     2761 actattcacc atagttcatg ttgtaatatt atactacaac ttcctaaact ctcagccaat
     2821 atatactgga tgaatgcccc cagctactca caccctcctt acaagactcc ctgcgctagc
     2881 tttacccaga tcacctttgc cacgcgtgaa ttcgctgagg gcgtttaaaa tcctcagcaa
     2941 ttcgaatttt aagctgcaga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta
     3001 caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc
     3061 gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg
     3121 tccactccca gttcaattac agctcttaaa attccgccac tagagcctct gctaaccatg
     3181 ttcatgcctt cttctctttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     3241 atcattttgg caaagaatta ttccgctagc gctaccggtc gccaccatgg tgagcaaggg
     3301 cgaggagacc acaatgggcg taatcaagcc cgacatgaag atcaagctga agatggaggg
     3361 caacgtgaat ggccacgcct tcgtgatcga gggcgagggc gagggcaagc cctacgacgg
     3421 caccaacacc atcaacctgg aggtgaagga gggagccccc ctgcccttct cctacgacat
     3481 tctgaccacc gcgttcgcct acggcaacag ggccttcacc aagtaccccg acgacatccc
     3541 caactacttc aagcagtcct tccccgaggg ctactcttgg gagcgcacca tgaccttcga
     3601 ggacaagggc atcgtgaagg tgaagtccga catctccatg gaggaggact ccttcatcta
     3661 cgagatacac ctcaagggcg agaacttccc ccccaacggc cccgtgatgc agaagaagac
     3721 caccggctgg gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga
     3781 cgtcaagcac aagctgctgc tggagggcgg cggccaccac cgcgttgact tcaagaccat
     3841 ctacagggcc aagaaggcgg tgaagctgcc cgactatcac tttgtggacc accgcatcga
     3901 gatcctgaac cacgacaagg actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg
     3961 caattccacc gacggcatgg acgagctgta caagtccgga ctcagatctc gagctgatcc
     4021 aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtagatccaa aaaagaagag
     4081 aaaggtagga tccaccggat ctagacccga gggcagagga agtcttctaa catgcggtga
     4141 cgtggaggag aatcccggcc cactagtcat ggccggcgat atgagagccg ctaacctgtg
     4201 gccctcccca ctgatgatca agagaagcaa gaagaactcc ctggccctga gcctgaccgc
     4261 tgatcagatg gtgtccgccc tgctggacgc cgagccccct atcctgtaca gcgagtacga
     4321 ccccaccagg cccttcagcg aggccagcat gatgggactg ctgacaaacc tggccgatcg
     4381 cgaactggtg catatgatca attgggccaa gcgcgtgccc ggattcgtgg acctgacact
     4441 gcacgaccag gtgcacctgc tggaatgcgc ttggctggaa attctgatga tcggcctcgt
     4501 gtggcggagc atggaacatc ctgtgaagct gctgttcgcc cccaatctgc tgctggaccg
     4561 caaccagggc aaatgcgtgg aagggatggt cgaaatcttt gatatgctgc tcgccaccag
     4621 cagcaggttc agaatgatga acctccaggg cgaagagttc gtctgcctga agtccatcat
     4681 cctgctgaac agcggcgtgt acaccttcct gtcctctacc ctgaagtccc tcgaggaaaa
     4741 ggatcacatc cacagggtgc tggataagat caccgatacc ctgatccatc tcatggctaa
     4801 ggccggactc acactccagc agcagcatca gagactcgct cagctgctgc tgatcctgag
     4861 ccacatccgc cacatgtcca acaagggaat ggaacacctc tactctatga agtgtaaaaa
     4921 cgtcgtcccc ctgtacgatc tgctgctcga ggccgccgat gcccacagac tgcacgcccc
     4981 tacaagcaga ggcggcgcta gcgtggaaga gacagaccag tcccacctgg ccaccgccgg
     5041 cagcacaagc agccacagcc tgcagaagta ctatatcacc ggcgaggccg agggcttccc
     5101 cgctacagct gtcgacaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga
     5161 tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt
     5221 ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa
     5281 gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt atcttctata
     5341 tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc agctaaacat
     5401 gcttcatcgt cggcccgggc tgccacgacc aagtgacagc aatgctgttt cactggttat
     5461 gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt
     5521 cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc gctgccagga
     5581 tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta tagccgaaat
     5641 tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt taatccatat
     5701 tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta gcctgggggt
     5761 aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc cgaataacta
     5821 cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca gccagctatc
     5881 aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg gcgctaagga
     5941 tgactccggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg
     6001 agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg gctggaccaa
     6061 tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg caatggtgcg
     6121 cctgctggaa gatggcgatc tcgagccatc tgctggagac atgagagctg ccaacctttg
     6181 gccaagcccg ctcatgatca aacgctctaa gaagaacagc ctggccttgt ccctgacggc
     6241 cgaccagatg gtcagtgcct tgttggatgc tgagcccccc atactctatt ccgagtatga
     6301 tcctaccaga cccttcagtg aagcttcgat gatgggctta ctgaccaacc tggcagacag
     6361 ggagctggtt cacatgatca actgggcgaa gagggtgcca ggctttgtgg atttgaccct
     6421 ccatgatcag gtccaccttc tagaatgtgc ctggctagag atcctgatga ttggtctcgt
     6481 ctggcgctcc atggagcacc cagtgaagct actgtttgct cctaacttgc tcttggacag
     6541 gaaccaggga aaatgtgtag agggcatggt ggagatcttc gacatgctgc tggctacatc
     6601 atctcggttc cgcatgatga atctgcaggg agaggagttt gtgtgcctca aatctattat
     6661 tttgcttaat tctggagtgt acacatttct gtccagcacc ctgaagtctc tggaagagaa
     6721 ggaccatatc caccgagtcc tggacaagat cacagacact ttgatccacc tgatggccaa
     6781 ggcaggcctg accctgcagc agcagcacca gcggctggcc cagctcctcc tcatcctctc
     6841 ccacatcagg cacatgagta acaaaggcat ggagcatctg tacagcatga agtgcaagaa
     6901 cgtggtgccc ctctatgacc tgctgctgga ggcggcggac gcccaccgcc tacatgcgcc
     6961 cactagccgt ggaggggcat ccgtggagga gacggaccaa agccacttgg ccactgcggg
     7021 ctctacttca tcgcattcct tgcaaaagta ttacatcacg ggggaggcag agggtttccc
     7081 tgccacagct taagatatca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     7141 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     7201 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     7261 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     7321 aaatcattta aaacatcaga atgagcattt ggtttagagt ttggcaacat atgcccatat
     7381 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     7441 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     7501 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     7561 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag
     7621 atccctcgac cagcggccgc taatactcaa gtacaatttt aatggagtac ttttttactt
     7681 ttactcaagt aagattctag ccagatactt ttacttttaa ttgagtaaaa ttttccctaa
     7741 gtacttgtac tttcacttga gtaaaatttt tgagtacttt ttacacctct gccgcggtgg
     7801 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcattaccct gttatcccta
     7861 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7921 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7981 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8041 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8101 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8161 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8221 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8281 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8341 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8401 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8461 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8521 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8581 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8641 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8701 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8761 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8821 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8881 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8941 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9001 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9061 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9121 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9181 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9241 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9301 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9361 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9421 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9481 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9541 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9601 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9661 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9721 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9781 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9841 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9901 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9961 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10021 aagtgccac
//
