LOCUS       pRRL.TRE3G.dsRED        8857 bp    DNA     circular UNA 10-JUL-2014
DEFINITION  .
ACCESSION   urn.local...1tj-3emcl0d
VERSION     urn.local...1tj-3emcl0d
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..232
                     /gene="RSV"
                     /label="RSV"
     LTR             233..414
                     /gene="RU5"
                     /label="RU5"
     misc_feature    415..432
                     /gene="PBS"
                     /label="PBS"
     misc_feature    569..933
                     /gene="dGag"
                     /label="dGag"
     misc_feature    1079..1320
                     /gene="RRE"
                     /label="RRE"
     misc_feature    1804..1926
                     /gene="PPT"
                     /label="PPT"
     promoter        1945..2320
                     /label="TRE3G"
     CDS             2344..3021
                     /label="dsRed"
     misc_feature    3029..3263
                     /note="Geneious type: misc_marker"
                     /label="miRE (empty)"
     promoter        3276..3784
                     /label="PGK"
     CDS             3807..4526
                     /label="Venus"
     misc_signal     4588..5176
                     /gene="WPRE"
                     /label="WPRE"
     LTR             5383..5616
                     /gene="SIN"
                     /label="SIN"
     misc_feature    5817..6023
                     /gene="SV40 pA/Ori"
                     /label="SV40\pA/Ori"
     misc_feature    6184..6642
                     /gene="F1 ORI"
                     /label="F1\ORI"
     CDS             6774..7631
                     /gene="Amp"
                     /label="Amp"
ORIGIN      
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     1921 ttttatcgat cctctagatt cgagtttact ccctatcagt gatagagaac gtatgaagag
     1981 tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga
     2041 acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat
     2101 cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag
     2161 tttactccct atcagtgata gagaacgtat aagctttagg cgtgtacggt gggcgcctat
     2221 aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga gcaattccac aacacttttg
     2281 tcttatacca actttccgta ccacttccta ccctcgtaaa gtcgagcttg cgttggatcc
     2341 accatggcct cctccgagaa cgtcatcacc gagttcatgc gcttcaaggt gcgcatggag
     2401 ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2461 ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     2521 atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc
     2581 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     2641 gaggacggcg gcgtggcgac cgtgacccag gactcctccc tgcaggacgg ctgcttcatc
     2701 tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtgat gcagaagaag
     2761 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     2821 gagacccaca aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtcc
     2881 atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga cgccaagctg
     2941 gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg caccgagggc
     3001 cgccaccacc tgttcctgta gcggccgcct cgactaggga taacagggta attgtttgaa
     3061 tgaggcttca gtactttaca gaatcgttgc ctgcacatct tggaaacact tgctgggatt
     3121 acttcgactt cttaacccaa cagaaggctc gagcaaccag aattcgagca attatcttgt
     3181 ttactaaaac tgaatacctt gctatctctt tgatacattt ttacaaagct gaattaaaat
     3241 ggtataaatt aaatcacttt tttcaattga cgcgtaattc taccgggtag gggaggcgct
     3301 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     3361 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     3421 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     3481 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     3541 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     3601 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     3661 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     3721 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     3781 ttcgacctgc agcccaagct tccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     3841 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     3901 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg
     3961 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct
     4021 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4081 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     4141 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4201 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     4261 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     4321 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4381 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     4441 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4501 tcggcatgga cgagctgtac aagtaagaat tgttcaattc ctgatcatcc tgcaggatgc
     4561 atgtcgactt cgaaacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     4621 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4681 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4741 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4801 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4861 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4921 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4981 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     5041 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     5101 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     5161 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     5221 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     5281 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     5341 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5401 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5461 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5521 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5581 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     5641 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     5701 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5761 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     5821 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5881 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5941 gtgaggaggc ttttttggag gcctaggctt ttgcgtcgag acgtacccaa ttcgccctat
     6001 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6061 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6181 cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6241 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6301 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6361 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6421 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6481 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6541 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6601 tttaacgcga attttaacaa aatattaacg tttacaattt cccaggtggc acttttcggg
     6661 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6721 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6781 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6841 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6901 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6961 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7021 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7081 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7141 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7201 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7261 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7321 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7381 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7441 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7501 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7561 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7621 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7921 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8461 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8521 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8581 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8641 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8701 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8761 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     8821 accctcacta aagggaacaa aagctggagc tgcaagc
//
