LOCUS       pRRL.TRE3G.dsRED       10339 bp    DNA     circular UNA 10-JUL-2014
DEFINITION  .
ACCESSION   urn.local...1to-3emcl0i
VERSION     urn.local...1to-3emcl0i
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..232
                     /gene="RSV"
                     /label="RSV"
     LTR             233..414
                     /gene="RU5"
                     /label="RU5"
     misc_feature    415..432
                     /gene="PBS"
                     /label="PBS"
     misc_feature    569..933
                     /gene="dGag"
                     /label="dGag"
     misc_feature    1079..1320
                     /gene="RRE"
                     /label="RRE"
     promoter        1945..2320
                     /label="TRE3G"
     CDS             2344..3021
                     /label="dsRED"
     misc_feature    3029..3376
                     /note="Geneious type: misc_marker"
                     /label="miRE (Ren.713)"
     promoter        3389..3897
                     /label="PGK"
     CDS             3920..4639
                     /label="Venus"
     misc_feature    4654..5225
                     /note="Geneious type: misc_signal"
                     /label="IRES"
     CDS             5246..6040
                     /label="Neo"
     misc_signal     6070..6658
                     /gene="WPRE"
                     /label="WPRE"
     LTR             6865..7098
                     /gene="SIN"
                     /label="SIN"
     misc_feature    7299..7505
                     /gene="SV40 pA/Ori"
                     /label="SV40\pA/Ori"
     misc_feature    7666..8124
                     /gene="F1 ORI"
                     /label="F1\ORI"
     CDS             8256..9113
                     /gene="Amp"
                     /label="Amp"
ORIGIN      
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     1921 ttttatcgat cctctagatt cgagtttact ccctatcagt gatagagaac gtatgaagag
     1981 tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga
     2041 acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat
     2101 cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag
     2161 tttactccct atcagtgata gagaacgtat aagctttagg cgtgtacggt gggcgcctat
     2221 aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga gcaattccac aacacttttg
     2281 tcttatacca actttccgta ccacttccta ccctcgtaaa gtcgagcttg cgttggatcc
     2341 accatggcct cctccgagaa cgtcatcacc gagttcatgc gcttcaaggt gcgcatggag
     2401 ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2461 ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     2521 atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc
     2581 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     2641 gaggacggcg gcgtggcgac cgtgacccag gactcctccc tgcaggacgg ctgcttcatc
     2701 tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtgat gcagaagaag
     2761 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     2821 gagacccaca aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtcc
     2881 atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga cgccaagctg
     2941 gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg caccgagggc
     3001 cgccaccacc tgttcctgta gcggccgcct cgactaggga taacagggta attgtttgaa
     3061 tgaggcttca gtactttaca gaatcgttgc ctgcacatct tggaaacact tgctgggatt
     3121 acttcgactt cttaacccaa cagaaggctc gagaaggtat attgctgttg acagtgagcg
     3181 caggaattat aatgcttatc tatagtgaag ccacagatgt atagataagc attataattc
     3241 ctatgcctac tgcctcggac ttcaaggggc tagaattcga gcaattatct tgtttactaa
     3301 aactgaatac cttgctatct ctttgataca tttttacaaa gctgaattaa aatggtataa
     3361 attaaatcac ttttttcaat tgacgcgtaa ttctaccggg taggggaggc gcttttccca
     3421 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     3481 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     3541 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     3601 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     3661 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     3721 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     3781 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     3841 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     3901 tgcagcccaa gcttccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     3961 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4021 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
     4081 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
     4141 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4201 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4261 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4321 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
     4381 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     4441 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4501 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     4561 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4621 ggacgagctg tacaagtaag aattgttcaa ttccgcccct ctccctcccc cccccctaac
     4681 gttactggcc gaagccgctt ggaataaggc cggtgtgtgt ttgtctatat gttattttcc
     4741 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     4801 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     4861 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     4921 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     4981 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     5041 agagtcaaat ggctctcctc aagcgtagtc aacaaggggc tgaaggatgc ccagaaggta
     5101 ccccattgta tgggaatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     5161 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     5221 acgatgataa gcttaccggt ccaccatgat tgaacaagat ggattgcacg caggttctcc
     5281 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5341 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5401 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     5461 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5521 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     5581 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5641 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5701 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5761 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     5821 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5881 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5941 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6001 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga tgtacgatgc atcgaaacgc
     6061 gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6121 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6181 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6241 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6301 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6361 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6421 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     6481 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6541 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6601 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     6661 ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     6721 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     6781 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6841 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6901 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6961 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7021 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7081 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7141 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7201 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7261 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7321 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7381 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7441 aggcctaggc ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg
     7501 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7561 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7621 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc
     7681 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7741 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7801 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7861 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7921 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7981 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     8041 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     8101 aaaatattaa cgtttacaat ttcccaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8161 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8221 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8281 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8341 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8401 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8461 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8521 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8581 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8641 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8701 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8761 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8821 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8881 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8941 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9001 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9061 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9121 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9421 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9901 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9961 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10021 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    10081 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    10141 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10201 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10261 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    10321 aaaagctgga gctgcaagc
//
