LOCUS       pRRL.TRE3G.dsRED       10293 bp    DNA     circular UNA 10-JUL-2014
DEFINITION  .
ACCESSION   urn.local...1tt-3emcl0p
VERSION     urn.local...1tt-3emcl0p
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..232
                     /gene="RSV"
                     /label="RSV"
     LTR             233..414
                     /gene="RU5"
                     /label="RU5"
     misc_feature    415..432
                     /gene="PBS"
                     /label="PBS"
     misc_feature    569..933
                     /gene="dGag"
                     /label="dGag"
     misc_feature    1079..1320
                     /gene="RRE"
                     /label="RRE"
     misc_feature    1804..1926
                     /gene="PPT"
                     /label="PPT"
     promoter        1945..2320
                     /label="TRE3G"
     CDS             2344..3021
                     /label="dsRED"
     misc_feature    3029..3376
                     /note="Geneious type: misc_marker"
                     /label="miRE (Ren.713)"
     promoter        3389..3897
                     /label="PGK"
     CDS             3920..4639
                     /label="Venus"
     misc_feature    4656..5225
                     /note="Geneious type: misc_signal"
                     /label="IRES"
     CDS             5232..5978
                     /label="rtTA3"
     misc_signal     6024..6612
                     /gene="WPRE"
                     /label="WPRE"
     LTR             6819..7052
                     /gene="SIN"
                     /label="SIN"
     misc_feature    7253..7459
                     /gene="SV40 pA/Ori"
                     /label="SV40\pA/Ori"
     misc_feature    7620..8078
                     /gene="F1 ORI"
                     /label="F1\ORI"
     CDS             8210..9067
                     /gene="Amp"
                     /label="Amp"
ORIGIN      
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     1921 ttttatcgat cctctagatt cgagtttact ccctatcagt gatagagaac gtatgaagag
     1981 tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga
     2041 acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat
     2101 cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag
     2161 tttactccct atcagtgata gagaacgtat aagctttagg cgtgtacggt gggcgcctat
     2221 aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga gcaattccac aacacttttg
     2281 tcttatacca actttccgta ccacttccta ccctcgtaaa gtcgagcttg cgttggatcc
     2341 accatggcct cctccgagaa cgtcatcacc gagttcatgc gcttcaaggt gcgcatggag
     2401 ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2461 ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     2521 atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc
     2581 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     2641 gaggacggcg gcgtggcgac cgtgacccag gactcctccc tgcaggacgg ctgcttcatc
     2701 tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtgat gcagaagaag
     2761 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     2821 gagacccaca aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtcc
     2881 atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga cgccaagctg
     2941 gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg caccgagggc
     3001 cgccaccacc tgttcctgta gcggccgcct cgactaggga taacagggta attgtttgaa
     3061 tgaggcttca gtactttaca gaatcgttgc ctgcacatct tggaaacact tgctgggatt
     3121 acttcgactt cttaacccaa cagaaggctc gagaaggtat attgctgttg acagtgagcg
     3181 caggaattat aatgcttatc tatagtgaag ccacagatgt atagataagc attataattc
     3241 ctatgcctac tgcctcggac ttcaaggggc tagaattcga gcaattatct tgtttactaa
     3301 aactgaatac cttgctatct ctttgataca tttttacaaa gctgaattaa aatggtataa
     3361 attaaatcac ttttttcaat tgacgcgtaa ttctaccggg taggggaggc gcttttccca
     3421 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     3481 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     3541 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     3601 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     3661 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     3721 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     3781 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     3841 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     3901 tgcagcccaa gcttccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     3961 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4021 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
     4081 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
     4141 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4201 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4261 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4321 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
     4381 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     4441 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4501 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     4561 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4621 ggacgagctg tacaagtaag aattgttcaa ttccgccccc cccccctaac gttactggcc
     4681 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     4741 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     4801 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     4861 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     4921 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     4981 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     5041 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     5101 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     5161 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     5221 tggccacaac catgagtaga ctggacaaga gcaaagtcat aaacggagct ctggaattac
     5281 tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
     5341 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa
     5401 tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag
     5461 actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg
     5521 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
     5581 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
     5641 tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg
     5701 aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt
     5761 tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
     5821 tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact
     5881 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
     5941 ctcttgacga ttttgacctt gacatgctcc ccgggtaatc cggactcgac ctgcagccaa
     6001 gcttatcgaa acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga
     6061 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6121 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6181 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6241 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6301 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6361 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     6421 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6481 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6541 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6601 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac
     6661 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     6721 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     6781 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6841 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     6901 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6961 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7021 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7081 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7141 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7201 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7261 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7321 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7381 ggaggctttt ttggaggcct aggcttttgc gtcgagacgt acccaattcg ccctatagtg
     7441 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     7501 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     7561 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcg
     7621 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7681 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7741 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7801 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7861 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7921 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7981 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8041 acgcgaattt taacaaaata ttaacgttta caatttccca ggtggcactt ttcggggaaa
     8101 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8161 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8221 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8281 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8341 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8401 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8461 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8521 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8581 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8641 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8701 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8761 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8821 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8881 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8941 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     9001 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     9061 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9121 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9181 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9241 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9301 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9361 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     9421 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9481 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9541 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9601 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9661 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9721 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9781 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9841 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9901 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9961 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
    10021 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
    10081 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    10141 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    10201 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
    10261 tcactaaagg gaacaaaagc tggagctgca agc
//
