LOCUS       Exported                5932 bp ds-DNA    circular SYN 01-MAR-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pLIC-SM-HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 25, 2018 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    124..500
                     /note="Tubulin Promotor"
                     /note="color: #339966"
     CDS             506..1168
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ccffcc"
                     /translation="MHEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFS
                     SLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVAN
                     MDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    1170..1481
                     /note="TgAntigenP30"
                     /note="color: #a6acb3"
     primer_bind     complement(1486..1502)
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     promoter        complement(1535..1553)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(1560..1576)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      1718..2173
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        2199..2303
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2304..3164
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2304 .. 2372 / #ccffcc / signal sequence
                        2: 2373 .. 3164 / #ccffcc
                     Cleavage site after base 2372"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3335..3923
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        4247..4277
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 4247 .. 4252 / #ffffff / -35
                        2: 4253 .. 4270 / #ffffff
                        3: 4271 .. 4277 / #ffffff / -10"
     protein_bind    4285..4301
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     4309..4325
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        4346..4364
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    4440..5489
                     /note="SMGFP_HA"
                     /note="color: #ff9900"
     CDS             4443..4469
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             4473..4499
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             4503..4529
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             5058..5084
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             5091..5117
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             5130..5156
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             5163..5189
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             5400..5426
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             5430..5456
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             5460..5486
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     misc_feature    5490..5895
                     /note="HXGPRT 3' UTR"
                     /note="color: #cc99ff"
     primer_bind     5914..5930
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 aagcttgata tgcatgtccg cgttcgtgaa attctctgat caagcggagt gatcaccaat
       61 catcgtctca gcgggatgac gttgcggcaa gggccggctc gcggtgggca gtcagatgcc
      121 gaacgtaact caggacggct tgcgctcatc gcagaacagg ggtggtgcct gcattgggtg
      181 cggttggtga tcctggttgg accggtggag atgcgcgcgc acgaagggga tgtgtcagaa
      241 acattttgtt tgttctctgt gaacttttag atgtgttaaa ggcggcgaat attagcagag
      301 agtcctcctt gttccattct ctcttgaatt tcgccctttc cttctctttg cgagtgtggt
      361 agagaacaag cactcgttcg ccgtccctga cgacgcaacc cgcgcagaag acatccacca
      421 aacggtgtta cacaatcacc ttgtgtgaag ttcttgcgga aaactactcg ttggcatttt
      481 ttcttgaatt ccctttttcg acaaaatgca tgagaaaaaa atcactggat ataccaccgt
      541 tgatatatcc caatcgcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
      601 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
      661 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
      721 ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
      781 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
      841 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
      901 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
      961 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     1021 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
     1081 tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     1141 ctgcgatgag tggcagggcg gggcttaatc aatcaccgtt gtgctcactt ctcaaatcga
     1201 caaaggaaac acacttcgtg cagcatgtgc cccattataa agaaactgag ttgttccgct
     1261 gtggcttgca ggtgtcacat ccacaaaaac cggccgactc taaataggag tgtttcgcag
     1321 caagcagcga aagtttatga ctgggtccga atctctgaac ggatgtgtgg cggacctggc
     1381 tgatgttgat cgccgtcgac acacgcgcca catgggtcaa tacacaagac agctatcagt
     1441 tgttttagtc gaaccggtta acacaattct tgcccccccg agggggatcc actagttcta
     1501 gagcggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta ttacaattca
     1561 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1621 cttgcagcac atcccccctt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1681 ccttcccaac agttgcgtag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     1741 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1801 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1861 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1921 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1981 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     2041 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     2101 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     2161 acgtttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2221 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2281 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2341 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2401 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2461 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2521 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2581 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2641 atggcatgac agtaagagaa ttatgcagtg ctgccataag catgagtgat aacactgcgg
     2701 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt tttcacaaca
     2761 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2821 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2881 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     2941 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3001 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     3061 cctcccgtat cgtagttatc tacacgacgg gcagtcaggc aactatggat gaacgaaata
     3121 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     3181 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3241 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3301 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3361 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3421 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3481 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3541 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3601 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3661 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3721 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3781 gcggcagggt cggaacagga gagcgcacga gggagcttcc aggggggaac gcctggtatc
     3841 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3901 caggggggcc gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3961 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4021 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4081 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4141 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     4201 gcaacgcaat taatgtgagt tacctcactc attaggcacc ccaggcttta cactttatgc
     4261 ttccggctcc tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     4321 atgaccatga ttacgccaag ctcggaatta accctcacta aagggaacaa aagctgggta
     4381 ccctgtactt ccaatccaat ttaattaaaa ttggaagtgg aggacgggaa ttccctagga
     4441 tgtaccctta tgatgtgccc gattatgctg gctaccctta tgatgttcct gattacgccg
     4501 gatatccgta tgacgtgcca gactacgcgg gaggtgtgag caagggagag gagttgttta
     4561 ccggcgtggt ccccatactg gtggagttgg atggcgacgt taatggacat aaattctcgg
     4621 tccgcggcga gggagaggga gacgccacca atggcaagct gacgcttaag ttcatttgca
     4681 cgactggaaa attgcctgtc ccctggccga ccctggtcac cacactgacc tacggagtgc
     4741 agtgcttctc ccgttaccca gaccacatga agcagcacga tttcttcaag agcgcaatgc
     4801 cggagggata cgtgcaagaa cggactattt ccttcaagga tgacggaacc tataagaccc
     4861 gtgcggaggt caaattcgag ggtgacaccc tggtgaaccg aattgaactc aaaggaatcg
     4921 atttcaagga ggatggaaat attctgggtc acaagctgga atacaacttc aacagccata
     4981 atgtgtacat tacggctgat aagcagaaga acggcattaa ggccaatttc aagatccgcc
     5041 acaacgttga gggtggatat ccctacgacg tgcccgatta tgcgggcgga tatccgtatg
     5101 atgttccaga ttatgctggt ggtggaggct atccctatga tgtccccgac tacgccggag
     5161 gttacccata cgatgtgccc gactacgctg gaggcgatgg cagcgtgcag ctggcagatc
     5221 attatcaaca gaataccccc ataggtgatg gccccgttct gcttccagat aatcactacc
     5281 tttccaccca gagcgtgctt tcgaaagacc cgaacgaaaa gcgtgatcac atggtcctgc
     5341 tggagtttgt gaccgcggct ggaatcaccc tgggtatgga cgaactctac aagggtggct
     5401 acccctacga tgtgccggat tacgctggat atccgtatga cgtaccggac tatgccggtt
     5461 acccgtacga tgtcccggac tacgcttaac atatgtagaa aagttgtaac gttagtaaac
     5521 gtaacacacg ggtataatgc catttaatgg cgccgtatat ttttcactcc ccctctccga
     5581 taggaggaaa aagacgatcc gtctgcgaat gtatattaca tcgaccggcc atagtacgag
     5641 aagtggtgcc ggtattgttc tgtccggggg atgtaccatc ctattttttg tatatgtcgt
     5701 tcttgtcggg gagcaacagc ccatcttccg gctattgtct cgtatcagtt gtgaaacaga
     5761 ctgcgttttg ccgaaaatga gtcctatgtc cgatggctat ttgtaccgct tcgtaccatt
     5821 tcgcttgtca gctgtaatca accgaattca tttggcgaga catgattccg attaaattct
     5881 ttactgcgcg tgctcggatc cactagttct actcgaggtc gacggtatcg at
//
