LOCUS       Exported                9217 bp ds-DNA     circular SYN 23-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Wilton R, Ahrendt AJ, Shinde S, Sholto-Douglas DJ, Johnson JL, 
            Brennan MB, Kemner KM.
  TITLE     A New Suite of Plasmid Vectors for Fluorescence-Based Imaging of 
            Root Colonizing Pseudomonads.
  JOURNAL   Front Plant Sci 2017;8:2242.
  PUBMED    29449848
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   R. Wilton
  TITLE     Direct Submission
  JOURNAL   Exported Apr 23, 2018 from SnapGene 4.1.7
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             194..880
                     /codon_start=1
                     /transl_table=11
                     /product="pVS1 resolvase"
                     /label=pVS1 resolvase
                     /note="ORF1; not required for stable maintenance"
                     /protein_id="AAD19683.1"
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
                     RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
                     TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
                     DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
                     RQEEQA"
     CDS             877..1092
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="ORF2; not required for stable maintenance"
                     /protein_id="AAD19684.1"
                     /translation="MKPHQDGQDEPFFITEEIEAEMIAAGYVFEPPAHVSTVRLHEILA
                     GLSDAKLAAWPASLAAEETERRRLKR"
     CDS             1179..1808
                     /codon_start=1
                     /transl_table=11
                     /product="pVS1 partitioning protein"
                     /label=pVS1 partitioning protein
                     /note="StaA; similar to IncC2"
                     /protein_id="AAD19685.1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1829..2044
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="ORF3; not required for stable maintenance"
                     /protein_id="AAD19686.1"
                     /translation="MSKSTNTLSAGRPSARSSKAATLASLADTPAMKRVNFQLPAEDHT
                     KLKMYAVRQGKTITELLSEYIAQLPE"
     CDS             2237..3310
                     /codon_start=1
                     /transl_table=11
                     /product="pVS1 replication protein"
                     /label=pVS1 replication protein RepA
                     /note="RepA"
                     /protein_id="AAD19687.1"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3376..3570
                     /label=pVS1 origin of replication
                     /note="pVS1 origin of replication"
     rep_origin      4262..5091
                     /label=p15A origin of replication
                     /note="p15A origin of replication"
     regulatory      5145..5411
                     /regulatory_class="terminator"
                     /note="T4 transcription terminator"
     CDS             complement(5445..5462)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(5475..6178)
                     /codon_start=3
                     /product="mRuby2 codon optimized for expression in P. 
                     fluorescens"
                     /label=mRuby2
                     /translation="KGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMRIK
                     VIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVVT
                     VMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHMAL
                     KVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKF
                     AGLGGGMDELYK"
     misc_feature    6194..6200
                     /label=T7 gene10 RBS
     promoter        complement(6250..6278)
                     /label=Pc Promoter
     CDS             complement(7219..7896)
                     /codon_start=1
                     /transl_table=11
                     /product="RK2 tetracycline repressor protein"
                     /label=RK2 TetR
                     /note="TetR"
                     /protein_id="AAD19689.1"
                     /translation="MFISDKVSSMTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGV
                     QQPALYWHFRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRD
                     GARIHAGTRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSD
                     AGERGGTVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRK
                     GDD"
     CDS             7900..9174
                     /codon_start=1
                     /transl_table=11
                     /product="RK2 tetracycline resistance protein"
                     /label=RK2 TetA
                     /note="TetA"
                     /protein_id="AAD19688.1"
                     /translation="MSTNLSVIKNPRVQSDQRRLVRRPDVKPNIPLIVILSTVALDAVG
                     IGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALVQFACAPVLGALSDRFGRRPILLVS
                     LAGATVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSA
                     CFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREAL
                     NPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFG
                     ILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGI
                     GMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAG
                     AALYLLCLPALRRGLWSGAGQRADR"
ORIGIN
        1 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
       61 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
      121 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
      181 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
      241 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
      301 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
      361 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
      421 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
      481 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
      541 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
      601 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
      661 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
      721 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
      781 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
      841 ggcagccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
      901 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
      961 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
     1021 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
     1081 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     1141 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     1201 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     1261 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     1321 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     1381 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     1441 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     1501 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     1561 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     1621 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     1681 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     1741 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     1801 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
     1861 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
     1921 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
     1981 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
     2041 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
     2101 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
     2161 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
     2221 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     2281 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     2341 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcagcc gctgatcgaa tccgcaaaga
     2401 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     2461 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     2521 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     2581 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     2641 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     2701 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     2761 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     2821 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     2881 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     2941 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
     3001 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     3061 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     3121 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     3181 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     3241 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     3301 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     3361 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     3421 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     3481 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     3541 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     3601 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     3661 tatcgcggcc gatggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     3721 cggacaagcc gcgccgtcgc cactcgaccg ccggcgctga ggtctgcctc gtgaagaagg
     3781 tgttgctgac tcataccagg caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3841 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3901 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3961 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4021 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4081 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4141 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4201 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4261 cttaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa
     4321 cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa
     4381 ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta
     4441 accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg
     4501 gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc
     4561 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
     4621 aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta
     4681 aaccgaaagg caggaacagg agagcgcacg agggagccgc cagggggaaa cgcctggtat
     4741 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg
     4801 tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg ccctctcact tccctgttaa
     4861 gtatcttcct ggcatcttcc aggaaatctc cgccccgttc gtaagccatt tccgctcgcc
     4921 gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaatat atcctgtatc
     4981 acatattctg ctgacgcacc ggtgcagcct tttttctcct gccacatgaa gcacttcact
     5041 gacaccctca tcagtgccaa catagtaagc cagtatacac tccgctagcg ctgaggtctg
     5101 cctcgtgaag aaggtgttgc tgactcatac caggtagtcc gaggagcttt atgcttgtaa
     5161 accgttttgt gaaaaaattt ttaaaataaa aaaggggacc tctagggtcc ccaattaatt
     5221 agtaatataa tctattaaag gtcattcaaa aggtcatcca ccggatcaat tcccctgctc
     5281 gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc cgatccttgg agcccttgcc
     5341 ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc ttgacccgca gttgcaaacc
     5401 ctcactgatc cgctagtccg aggcctcgag atgcttaaag cttagtgatg gtgatgatga
     5461 tgtgcggccg cttatttata caactcatcc ataccaccgc ccaagcccgc aaatttggcc
     5521 acagcgtgtt cacgttgcac gacgaacatc tcgttgtcgc tttcttccag gcgctccaaa
     5581 cgatgatcga ctgcgtgaat cccgggcatt ttgatattgc caaccgtctt tttggaacga
     5641 taagtagtga cgaaactgca cgacaaatgg ccgccgccgt ccactttcaa cgccatgtga
     5701 gtatagccgc gcaggccacc gtcagcggga tacatcattt cggtattcgg ttcccaacct
     5761 ttggtcttct tttgcatcac agggccgtta ctcggaaagt taacaccgcg gacttgcaca
     5821 tgatacacca ggcaaccatc ttcgagggaa gtgtcttgca tgacagtaac gacgccaccg
     5881 tcctcgtaac gggtaacccg ttcccacgtg aaaccttctg gaaaggactg cttgaagaaa
     5941 tccgggatgc ccttggggta cttgataaac gtgcgcgagc cgtacataaa ggaggtcgcg
     6001 agaatatcga atgcgaaagg gaggggcccg ccttcaatca ccttaatccg catggtttgg
     6061 gtgcccatgt aggggttgcc ttcgccctcg ccggtgcatt tgaattggtg accgttcacg
     6121 ctaccttcca tcaccacttt catgcgcatg ttttctttga tcagttcttc acctttggaa
     6181 ttcatatgta tatctccttc ttaaagttaa acaaaattat ttcttgaacg ggttcgctac
     6241 ctgcattaca gcctacagac cgagaaggct tatgtctact gggccaaggc atttgtgttg
     6301 tggacggccc gcagccttgg tgggtttcga catccgcgcg aaatggggca agctgaagtc
     6361 gagggttttc tgaccatgct cgccaccgag aagcaagtgg cgccggccac ccaccggcag
     6421 gcgctcaacg cgctgttgtt cttgtatcgg caacgcgtcg acgtcatatg gatccgatat
     6481 cgccgtggcg gccagctttt gccattctca ccggattcag tcgtcactca tggtgatttc
     6541 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     6601 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     6661 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     6721 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     6781 taacactggc agagcattac gctgacttga cgggacggcg gctttgttga ataaatcgaa
     6841 cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg
     6901 caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
     6961 gctccctcac tttctggctg gatgatgggg cgattcaggc ctggtatgag tcagcaacac
     7021 cttcttcacg aggcagacct cagcgccaga aggccgccag agaggccgag cgcggccgtg
     7081 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     7141 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     7201 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     7261 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     7321 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     7381 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     7441 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     7501 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     7561 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     7621 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     7681 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     7741 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     7801 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     7861 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     7921 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     7981 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     8041 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     8101 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     8161 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     8221 gccactgtcg actacgccat catggcgaca gcgcctttcc tttgggttct ctatatcggg
     8281 cggatcgtgg ccggcatcac cggggcgact ggggcggtag ccggcgctta tattgccgat
     8341 atcactgatg gcgatgagcg cgcgcggcac ttcggcttca tgagcgcctg tttcgggttc
     8401 gggatggtcg cgggacctgt gctcggtggg ctgatgggcg gtttctcccc ccacgctccg
     8461 ttcttcgccg cggcagcctt gaacggcctc aatttcctga cgggctgttt ccttttgccg
     8521 gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg ctctcaaccc gctcgcttcg
     8581 ttccggtggg cccggggcat gaccgtcgtc gccgccctga tggcggtctt cttcatcatg
     8641 caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt tcggcgagga tcgctttcac
     8701 tgggacgcga ccacgatcgg catttcgctt gccgcatttg gcattctgca ttcactcgcc
     8761 caggcaatga tcaccggccc tgtagccgcc cggctcggcg aaaggcgggc actcatgctc
     8821 ggaatgattg ccgacggcac aggctacatc ctgcttgcct tcgcgacacg gggatggatg
     8881 gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg gaatgccggc gctgcaagca
     8941 atgttgtcca ggcaggtgga tgaggaacgt caggggcagc tgcaaggctc actggcggcg
     9001 ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca cggcgatcta tgcggcttct
     9061 ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg ccctctactt gctctgcctg
     9121 ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac gagccgatcg ctgatcgtgg
     9181 aaacgatagg ggccgctcta gtactagcta gaactat
//
