LOCUS       Exported                5859 bp ds-DNA     circular SYN 23-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Wilton R, Ahrendt AJ, Shinde S, Sholto-Douglas DJ, Johnson JL, 
            Brennan MB, Kemner KM.
  TITLE     A New Suite of Plasmid Vectors for Fluorescence-Based Imaging of 
            Root Colonizing Pseudomonads.
  JOURNAL   Front Plant Sci 2017;8:2242.
  PUBMED    29449848
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   R. Wilton
  TITLE     Direct Submission
  JOURNAL   Exported Apr 23, 2018 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /note="right end of Tn7"
     regulatory      629..723
                     /regulatory_class="terminator"
                     /note="bacteriophage lambda T0"
     regulatory      820..917
                     /regulatory_class="terminator"
                     /note="Escherichia coli rrnB T1"
     misc_feature    958..1005
                     /note="FRT; Flp recombinase target site"
     gene            complement(1135..1668)
                     /gene="aacC1"
                     /label=aacC1
     CDS             complement(1135..1668)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyl transferase"
                     /label=aacC1
                     /protein_id="AAT11770.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     misc_feature    1926..1973
                     /note="FRT; Flp recombinase target site"
     terminator      2043..2309
                     /label=T4 transcription terminator
                     /note="T4 transcription terminator"
     CDS             complement(2343..2360)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2373..3089)
                     /codon_start=1
                     /product="mTurquoise2 codon optimized for expression in P. 
                     fluorescens"
                     /label=mTurquoise2
                     /note="Expression optimized for Pseudomonas Fluorescens 
                     (BH)"
                     /translation="MNSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    3098..3104
                     /label=T7 gene10 RBS
     promoter        complement(3154..3182)
                     /label=Pc Promoter
     mobile_element  3376..3541
                     /mobile_element_type="transposon:Tn7L"
                     /note="left end of Tn7"
     misc_feature    3693..3960
                     /note="oriT; origin of transfer"
     gene            complement(4799..5659)
                     /gene="bla"
                     /label=bla
     CDS             complement(4799..5659)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAT11771.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggcgttgtga
     1081 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt
     1141 ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata
     1201 gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag
     1261 cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg
     1321 ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct caaacctggg
     1381 cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat
     1441 gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta
     1501 ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac
     1561 ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt
     1621 tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc
     1681 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     1741 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     1801 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
     1861 tacgaaccga acaggcttat gtcaattcga tccattgctg ttgacaaagg gaatcagggg
     1921 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     1981 ttttgaagct aattcgatca tgcatgagct ctgttgctga ctcataccag gctagtccga
     2041 ggagctttat gcttgtaaac cgttttgtga aaaaattttt aaaataaaaa aggggacctc
     2101 tagggtcccc aattaattag taatataatc tattaaaggt cattcaaaag gtcatccacc
     2161 ggatcaattc ccctgctcgc gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg
     2221 atccttggag cccttgccct cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt
     2281 gacccgcagt tgcaaaccct cactgatccg ctagtccgag gcctcgagat gcttaaagct
     2341 tagtgatggt gatgatgatg tgcggccgct tacttgtaca gctcgtccat gcccagggtg
     2401 atgccggccg cggtcacgaa ctccagcaga accatgtggt cgcgtttttc gttaggatct
     2461 ttactgagtt tactttgggt ggagaggtaa tggttgtcag gcagcagaac ggggccgtcg
     2521 ccgatggggg tattttgttg ataatggtct gcgagttgaa caccgccgtc ttcgatatta
     2581 tggcggattt taaaattagc tttgatgccg tttttctgtt tgtcagcggt gatgtaaacg
     2641 ttgtcggaga aatagttgta ttcgagtttg tggcccagga tgttaccgtc ttccttaaaa
     2701 tcaatgccct tcagttcgat gcggttgacc agggtatcgc cttcgaattt cacttcggcc
     2761 cgagttttgt agttgccgtc gtctttgaag aaaatggtgc gttcttgaac atagccttct
     2821 ggcatggcgc ttttgaagaa atcgtgttgt ttcatatggt cggggtaacg ggcaaagcat
     2881 tggacgcccc agctcaaggt ggtcacgagg gtaggccaag gaacagggag tttgccggta
     2941 gtgcagataa atttcagggt cagtttgccg taagttgcat cgccctcgcc ttcgccgctc
     3001 acggaaaatt tatggccatt cacgtcgcca tcgagttcca cgaggattgg cacaacgccg
     3061 gtaaagagct cttcgccctt ggaattcata tgtatatctc cttcttaaag ttaaacaaaa
     3121 ttatttcttg aacgggttcg ctacctgcat tacagcctac agaccgagaa ggcttatgtc
     3181 tactgggcca aggcatttgt gttgtggacg gcccgcagcc ttggtgggtt tcgacatccg
     3241 cgcgaaatgg ggcaagctga agtcgagggt tttctgacca tgctcgccac cgagaagcaa
     3301 gtggcgccgg ccacccaccg gcaggcgctc aacgcgctgt tgttcttgta tcggctcgcg
     3361 aaggccttgc aggccaacca gataagtgaa atctagttcc aaactatttt gtcattttta
     3421 attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta
     3481 aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac
     3541 agcggggcat ttttcttcct gttatgtttg ggcgctgcat taatgaatcg gccaacgcgc
     3601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg acccgctgcg
     3661 ctcggtcgtt cggctgcggc gagcggtatc agagcttatc ggccagcctc gcagagcagg
     3721 attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc
     3781 gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc
     3841 tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa
     3901 aatcgctata atgaccccga agcagggtta tgcagcggaa agtatacctt aaggaatccc
     3961 ctgataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4081 gacgctcgag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4441 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5401 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5761 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     5821 taacctataa aaataggcgt atcacgaggc cctttcgtc
//
