LOCUS       pAAV-hSynapsin1-        5986 bp    DNA     linear       25-APR-2018
FEATURES             Location/Qualifiers
     misc_feature    945..951
                     /note="gcamp3.X"
     misc_feature    449..920
                     /note="synapsin promoter"
     misc_feature    443..448
                     /note="BglII"
     misc_feature    2510..2530
                     /note="Mao 115r"
     misc_feature    2374..2962
                     /note="WPRE"
     misc_feature    2996..3227
                     /note="SV40"
     misc_feature    3256..3422
                     /note="ITR"
     misc_feature    260..426
                     /note="ITR"
     misc_feature    952..1011
                     /note="GAP43 Sequence"
     misc_feature    1012..2361
                     /note="GCaMP6m"
     misc_feature    1012..1119
                     /note="PRSET Leader"
     misc_feature    1459..1476
                     /note="Linker"
     misc_feature    1120..1182
                     /note="RS20 Peptide"
     misc_feature    1918..2358
                     /note="CaM 2-148"
     misc_feature    2140..2145
                     /note="M76G,K77S"
     source          1..5986
                     /dnas_title="pAAV-hSynapsin1-axon-GCaMP6m"
ORIGIN      
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaAGATCTct gcagagggcc ctgcgtatga gtgcaagtgg
      481 gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac cccgacccac
      541 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      601 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      661 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      721 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      781 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      841 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
      901 cgtgcctgag agcgcagtcg aattcaagct gctagcaagg atccCGCCAC CATGCTGTGC
      961 TGTATGAGAA GAACCAAACA GGTTGAAAAG AATGATGAGG ACCAAAAGAT TGGTTCTCAT
     1021 CATCATCATC ATCATGGTAT GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG
     1081 TACGACGATG ACGATAAGGA TCTCGCCACC ATGGTCGACT CATCACGTCG TAAGTGGAAT
     1141 AAGACAGGTC ACGCAGTCAG AGCTATAGGT CGGCTGAGCT CACTCGAGAA CGTCTATATC
     1201 AAGGCCGACA AGCAGAAGAA CGGCATCAAG GCGAACTTCA AGATCCGCCA CAACATCGAG
     1261 GACGGCGGCG TGCAGCTCGC CTACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC
     1321 GTGCTGCTGC CCGACAACCA CTACCTGAGC GTGCAGTCCA AACTTTCGAA AGACCCCAAC
     1381 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC
     1441 ATGGACGAGC TGTACAAGGG CGGTACCGGA GGGAGCATGG TGAGCAAGGG CGAGGAGCTG
     1501 TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC
     1561 AGCGTGTCCG GCGAGGGTGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC
     1621 TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC
     1681 GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC
     1741 ATGCCCGAAG GCTACATCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG
     1801 ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC
     1861 ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CCTGCCGGAC
     1921 CAACTGACTG AAGAGCAGAT CGCAGAATTT AAAGAGGCTT TCTCCCTATT TGACAAGGAC
     1981 GGGGATGGGA CAATAACAAC CAAGGAGCTG GGGACGGTGA TGCGGTCTCT GGGGCAGAAC
     2041 CCCACAGAAG CAGAGCTGCA GGACATGATC AATGAAGTAG ATGCCGACGG TGACGGCACA
     2101 ATCGACTTCC CTGAGTTCCT GACAATGATG GCAAGAAAAg gGAgcTACAG GGACACGGAA
     2161 GAAGAAATTA GAGAAGCGTT CGGTGTGTTT GATAAGGATG GCAATGGCTA CATCAGTGCA
     2221 GCAGAGCTTC GCCACGTGAT GACAAACCTT GGAGAGAAGT TAACAGATGA AGAGGTTGAT
     2281 GAAATGATCA GGGAAGCAGA CATCGATGGG GATGGTCAGG TAAACTACGA AGAGTTTGTA
     2341 CAAATGATGA CAGCGAAGTg AAAGCTTatc gataatcaac ctctggatta caaaatttgt
     2401 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2461 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2521 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2581 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2641 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2701 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2761 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     2821 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2881 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2941 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gacccgggcg gccgcttcga
     3001 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     3061 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3121 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     3181 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctt
     3241 cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc
     3301 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     3361 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     3421 cagccttaat taacctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3481 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3541 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     3601 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3661 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3721 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3781 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     3841 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3901 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     3961 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4021 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     4081 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4141 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4201 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4261 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4321 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4381 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4441 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4501 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4561 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4621 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4681 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4741 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4801 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4861 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4921 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4981 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5041 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5101 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5161 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5221 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5281 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5341 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5401 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5461 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5521 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5581 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5641 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5701 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5761 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5821 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5881 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5941 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//

