LOCUS       pAAV-hSynapsin1-        5989 bp    DNA     circular     25-APR-2018
FEATURES             Location/Qualifiers
     misc_feature    948..954
                     /note="gcamp3.X"
     misc_feature    449..920
                     /note="synapsin promoter"
     misc_feature    443..448
                     /note="BglII"
     misc_feature    2513..2533
                     /note="Mao 115r"
     misc_feature    2377..2965
                     /note="WPRE"
     misc_feature    2999..3230
                     /note="SV40"
     misc_feature    3259..3425
                     /note="ITR"
     misc_feature    260..426
                     /note="ITR"
     misc_feature    1015..1122
                     /note="PRSET Leader"
     misc_feature    1021..1038
                     /note="6xHisTag"
     misc_feature    1186..1461
                     /note="cpGFP 147-238"
     misc_feature    1462..1479
                     /note="Linker"
     misc_feature    1480..1920
                     /note="cpGFP 1-146"
     misc_feature    1123..1185
                     /note="RS20 Peptide"
     misc_feature    1921..2361
                     /note="CaM 2-148"
     misc_feature    958..1014
                     /note="GAP43 Sequence"
     source          1..5989
                     /dnas_title="pAAV-hSynapsin1-axon-GCaMP6f"
ORIGIN      
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaAGATCTct gcagagggcc ctgcgtatga gtgcaagtgg
      481 gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac cccgacccac
      541 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      601 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      661 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      721 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      781 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      841 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
      901 cgtgcctgag agcgcagtcg aattcaagct gctagcaagg atccaccCGC CACCatgCTG
      961 TGCTGTATGA GAAGAACCAA ACAGGTTGAA AAGAATGATG AGGACCAAAA GATTggttct
     1021 catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat
     1081 ctgtacgacg atgacgataa ggatctcgcc accatggtcg actcatcacg tcgtaagtgg
     1141 aataagacag gtcacgcagt cagagctata ggtcggctga gctcactcga gaacgtctat
     1201 atcaaggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
     1261 gaggacggcg gcgtgcagct cgcctaccac taccagcaga acacccccat cggcgacggc
     1321 cccgtgctgc tgcccgacaa ccactacctg agcgtgcagt ccaaactttc gaaagacccc
     1381 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1441 ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagcaa gggcgaggag
     1501 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1561 ttcagcgtgt ccggcgaggg tgagggcgat gccacctacg gcaagctgac cctgaagttc
     1621 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1681 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1741 gccatgcccg aaggctacat ccaggagcgc accatcttct tcaaggacga cggcaactac
     1801 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1861 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacctgccg
     1921 gaccaactga ctgaagagca gatcgcagaa tttaaagagg aattctccct atttgacaag
     1981 gacggggatg ggacaataac aaccaaggag ctggggacgg tgatgcggtc tctggggcag
     2041 aaccccacag aagcagagct gcaggacatg atcaatgaag tagatgccga cggtgacggc
     2101 acaatcgact tccctgagtt cctgacaatg atggcaagaa aaatgaaata cagggacacg
     2161 gaagaagaaa ttagagaagc gttcggtgtg tttgataagg atggcaatgg ctacatcagt
     2221 gcagcagagc ttcgccacgt gatgacaaac cttggagaga agttaacaga tgaagaggtt
     2281 gatgaaatga tcagggaagc agacatcgat ggggatggtc aggtaaacta cgaagagttt
     2341 gtacaaatga tgacagcgaa gtaaAAGCTT atcgataatc aacctctgga ttacaaaatt
     2401 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2461 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2521 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2581 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     2641 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     2701 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     2761 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     2821 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     2881 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     2941 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgacccgg gcggccgctt
     3001 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     3061 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     3121 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     3181 atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     3241 cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta actacaagga
     3301 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     3361 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     3421 gcgcagcctt aattaaccta attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     3481 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     3541 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     3601 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3661 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3721 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3781 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3841 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3901 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     3961 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4021 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     4081 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4141 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4201 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4261 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4321 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4381 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4441 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4501 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4561 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4621 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4681 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4741 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4801 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4861 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4921 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4981 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5041 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5101 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5161 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5221 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5281 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5341 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5401 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5461 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5521 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5581 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5641 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5701 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5761 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5821 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5881 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5941 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//

