LOCUS       Exported                3595 bp ds-DNA     circular SYN 26-APR-2018
DEFINITION  Ligation of inverted pSP73TEL* into 
            pENTR/D/hH1_DeltaNMP265-DeltaSalI+Linker*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 26, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
COMMENT     Vector molecule:  pENTR/D/hH1_DeltaNMP265-DeltaSalI+Linker*
               Fragment ends:
               Fragment size: 2727
            Insert molecule:  pSP73TEL*
               Fragment ends:
               Fragment size: 868
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    268..295
                     /label=T2
     misc_feature    427..470
                     /label=T1
     misc_feature    537..553
                     /label=M13fw-Lab
                     /note="Labor Primer"
     misc_feature    537..552
                     /label=M13fw
     misc_feature    569..651
                     /label=attL1'
     misc_feature    complement(652..668)
                     /label=attL1'
     misc_feature    697..796
                     /label=hH1 prom
     misc_feature    802
                     /label=TSP
     misc_feature    813..830
                     /label=exoTerra-Primer_fwd
     misc_feature    complement(851..1658)
                     /label=Tel-Region
     misc_feature    1695..1699
                     /label=polIII TS
                     /note="termination signal"
     misc_feature    1720..1729
                     /label=attL2'
     misc_feature    1730..1819
                     /label='attL2
     misc_feature    1856..1874
                     /label=M13rev
                     /note="labor Primer"
     misc_feature    1860..1876
                     /label=M13r
     misc_feature    1989..2798
                     /label=Kan
     misc_feature    2919..3592
                     /label=pUC ori
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttt aaaggaacca attcagtcga tcgactggat ccatatttgc atgtcgctat
      721 gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc
      781 tgtatgagac cactcgcggc cagtggaatt gtcgatcccc gggtaccgag ctcgaattcc
      841 gcggaattcc ggttggggtt ggggttgggg ttggggttgg ggttagggtt ggggttaggg
      901 ttagcgttag ggttagggtt agggttgggg ttagggttag ggttagggtt agggttaggg
      961 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1021 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1081 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1141 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1201 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1261 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1321 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1381 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1441 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1501 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1561 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1621 ttagggttag ggttagggtt agggttaggg ttagggttcg gaattcatcg atgatatcag
     1681 atcgacaatt ccactttttt taattctcga gatatctaga cccagctttc ttgtacaaag
     1741 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1801 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     1861 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1921 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     1981 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     2041 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     2101 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     2161 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     2221 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     2281 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2341 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2401 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2461 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2521 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2581 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2641 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2701 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2761 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     2821 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     2881 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2941 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3001 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3061 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3301 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     3361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
