LOCUS       S11-pAAV-hSynDIO        5798 bp    DNA     circular     14-APR-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|731175358|
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FEATURES             Location/Qualifiers
     misc_feature    756..789
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=lox
     misc_feature    complement(1764..1766)
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
                     /vntifkey="21"
                     /label=ATG
     misc_feature    complement(1868..1901)
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=loxP
     misc_feature    3868..3890
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="21"
                     /label=pGEX_3_primer
     rep_origin      3294..3600
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="33"
                     /label=f1_origin
     CDS             4119..4979
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="4"
                     /label=ORF\frame\1
     misc_feature    671..704
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=Lox
     rep_origin      5134..5753
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_feature    complement(1761..1787)
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
                     /vntifkey="21"
                     /label=AGV331-NheI-Cheta-FWD
     terminator      2544..3022
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="43"
                     /label=hGH_PA_terminator
     misc_feature    complement(829..1763)
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
                     /vntifkey="21"
                     /label=Cheta
     promoter        4049..4077
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    complement(1784..1817)
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(802..828)
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
                     /vntifkey="21"
                     /label=HA
     misc_feature    1928..2515
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    157..641
                     /vntifkey="21"
                     /label=hSynapsin\promoter
BASE COUNT     1286 a      1583 c      1514 g      1415 t 
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt 
       61 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc 
      121 aactccatca ctaggggttc ctgcggccgc acgcgtgtgt ctagactgca gagggccctg 
      181 cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac 
      241 gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct 
      301 atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc 
      361 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga 
      421 aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg 
      481 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca 
      541 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg 
      601 cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaga aggtaccgga tcctctagag 
      661 tcgactccgg aataacttcg tataggatac tttatacgaa gttatgcaga atggtagctg 
      721 gattgtagct gctattagca atatgaaacc tcttaataac ttcgtatagc atacattata 
      781 cgaagttatg gcgcgtaatt aggcgtagtc gggcacgtcg taggggtaca tggtggcggc 
      841 cgctggcacg gctccggcct cggcttcgtc ttcgacgaga gtctcgacct cgatctccgt 
      901 tccgccgatg ttcagtttgg tggttttgcg gatatctccg tgaatcaata tgtgctcgtg 
      961 gatcaggacg cgcaggtagt gtcccaacaa cccccaacaa tttttactca tcagatcaat 
     1021 aatcgtgtga cctacggtgg agccatagac gctcaggacg ccaaaacctt cgggccccaa 
     1081 aatgaagaga attgggaaca taccccagct cacgaaaaac agccatgcca tgccggtcac 
     1141 gacctggcgg caccgaccct ttggcacagt atgataaccc tcgatatatg ctttggcggc 
     1201 gtgaaaaaat gtgttcgcgc catagcacaa tccaagacaa aagaagatga ctttaacata 
     1261 gccggttgcc atggcgctgg tagcccccca cacgatagtc ccgatgtctg agacaaggag 
     1321 tcccatggtt ctcctgctgt agtcgttgct caggccggtg aggttgctca ggcggataag 
     1381 gatgacagga caagtgagca gccaggttgc atagcgcagc cactgcaccc ggtgtcctgt 
     1441 ggcaaggtag agcatagagg gattcttaaa ctcaaaaaag aactcgagaa tcaccttaac 
     1501 catttcaatg gcgcacacat agatctcctc ccagccgcat gtagatttcc aggtttggta 
     1561 ggcatagaac atcagcagca aaatgctgaa tcctgctgca agccactgca ggacatttga 
     1621 cgcggtctga gcgccgttcg tgccgcgaga ttcaatccat ccggcacagt aacattgatc 
     1681 ctcagggacc aggacggacc cgttcaccac cacaggatta gtaacgaaca aaagttcgcg 
     1741 tccgacggca gacaaagcgc cgccatagtc catggtggct agcataactt cgtataaagt 
     1801 atcctatacg aagttatttg ccttaaccca gaaattatca ctgttattct ttagaatggt 
     1861 gcaaagaata acttcgtata atgtatgcta tacgaagtta tgaattcgat atcaagctta 
     1921 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 
     1981 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 
     2041 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 
     2101 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 
     2161 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 
     2221 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 
     2281 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc 
     2341 tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 
     2401 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 
     2461 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat 
     2521 accgagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct 
     2581 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg 
     2641 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg 
     2701 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag 
     2761 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat 
     2821 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta 
     2881 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc 
     2941 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac 
     3001 cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg 
     3061 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag 
     3121 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag 
     3181 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc 
     3241 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa 
     3301 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     3361 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     3421 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     3481 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     3541 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     3601 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct 
     3661 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 
     3721 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc 
     3781 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat 
     3841 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt 
     3901 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg 
     3961 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa 
     4021 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 
     4081 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 
     4141 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 
     4201 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 
     4261 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 
     4321 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 
     4381 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 
     4441 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 
     4501 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 
     4561 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 
     4621 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 
     4681 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 
     4741 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 
     4801 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 
     4861 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 
     4921 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 
     4981 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 
     5041 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 
     5101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 
     5161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 
     5221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 
     5281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 
     5341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 
     5401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 
     5461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 
     5521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 
     5581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 
     5641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 
     5701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 
     5761 tacggttcct ggccttttgc tggccttttg ctcacatg 
//
