LOCUS       pAAV-hSynDIO-mCa        6897 bp    DNA     circular     27-MAR-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|729545430|
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FEATURES             Location/Qualifiers
     misc_feature    1887..1889
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
                     /vntifkey="21"
                     /label=TAA
     misc_feature    756..789
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=lox
     misc_feature    1857..1886
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
                     /vntifkey="21"
                     /label=MYC
     misc_feature    complement(2863..2865)
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
                     /vntifkey="21"
                     /label=ATG
     misc_feature    complement(2967..3000)
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=loxP
     misc_feature    1890..1897
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
                     /vntifkey="21"
                     /label=PacI
     misc_feature    795..800
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
                     /vntifkey="21"
                     /label=Kozak
     misc_feature    4967..4989
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="21"
                     /label=pGEX_3_primer
     rep_origin      4393..4699
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="33"
                     /label=f1_origin
     CDS             5218..6078
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="4"
                     /label=ORF\frame\1
     misc_feature    1871..1944
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
                     /vntifkey="21"
                     /label=AGV332\::\oMyc_PacI_HA_Cheta-2A_REV
     misc_feature    671..704
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=Lox
     rep_origin      6233..6852
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_feature    complement(2860..2886)
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
                     /vntifkey="21"
                     /label=AGV331-NheI-Cheta-FWD
     terminator      3643..4121
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="43"
                     /label=hGH_PA_terminator
     misc_feature    790..797
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
                     /vntifkey="21"
                     /label=AscI
     misc_feature    complement(1928..2862)
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
                     /vntifkey="21"
                     /label=Cheta
     promoter        5148..5176
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    complement(2883..2916)
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(1901..1927)
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
                     /vntifkey="21"
                     /label=HA
     misc_feature    3027..3614
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    157..641
                     /vntifkey="21"
                     /label=hSynapsin\promoter
     misc_feature    801..1856
                     /vntifkey="21"
                     /label=mCARv2
     primer          1827..1909
                     /vntifkey="27"
                     /label=mCARv2-R
     primer          785..823
                     /vntifkey="27"
                     /label=AscI-mCar-F
BASE COUNT     1587 a      1858 c      1792 g      1660 t 
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt 
       61 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc 
      121 aactccatca ctaggggttc ctgcggccgc acgcgtgtgt ctagactgca gagggccctg 
      181 cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac 
      241 gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct 
      301 atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc 
      361 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga 
      421 aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg 
      481 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca 
      541 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg 
      601 cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaga aggtaccgga tcctctagag 
      661 tcgactccgg aataacttcg tataggatac tttatacgaa gttatgcaga atggtagctg 
      721 gattgtagct gctattagca atatgaaacc tcttaataac ttcgtatagc atacattata 
      781 cgaagttatg gcgcgccacc atggctcgcc tactgtgctt cgtgctcttg tgcgggatcg 
      841 cggatttcac cagtggtttg agcatcacta cacccgaaca gaggatcgaa aaagccaaag 
      901 gggaaactgc gtatctacca tgcaagttta ctctcagtcc cgaagaccag ggaccactgg 
      961 acattgaatg gctgatatcc ccgtctgata accagatagt ggatcaagtg atcattttgt 
     1021 attctggaga caaaatttat gataactact atccggatct gaaaggacgg gtacatttta 
     1081 cgagtaacga tgtcaagtct ggcgacgcat ctataaatgt gaccaacctg cagctgtcgg 
     1141 acattggcac ttaccagtgc aaagtgaaga aagcccctgg ggttgcaaat aagaaattcc 
     1201 tgctgaccgt tcttgttaag ccttcaggta caagatgctt cgtggatgga tcggaagaga 
     1261 ttggaaatga cttcaagcta aaatgtgaac ccaaggaagg ctcccttcca ctacagtttg 
     1321 aatggcagaa actgtcggac tcccagacaa tgcctacgcc atggctggca gaaatgacgt 
     1381 caccagttat atctgtgaag aacgccagtt ctgagtattc tgggacatac agctgcacgg 
     1441 ttcaaaacag agtgggctct gaccagtgta tgctgcgact agacgttgtc ccaccctcca 
     1501 accgagccgg aacgatcgcg ggcgccgtca tagggacgct gctggccctt gtgctcatcg 
     1561 gggccatcct cttctgctgt cacaggaaac gcagagaaga gaagtacgag aaggaagttc 
     1621 atcatgatat cagggaagat gtgcctcctc caaagagtcg gacatccact gccaggagct 
     1681 atattggcag caaccattcc tccctgggat ccatgtcccc ctctaacatg gaggggtatt 
     1741 ccaagacgca gtataaccaa gtccccagtg aagactttga acgtgcgcct cagagcccga 
     1801 ctctggcacc cgctaagttc aagtacgctt acaagaccga tggcattaca gtggtagagc 
     1861 agaagctgat cagcgaggag gacctgtaat taattaatta ggcgtagtcg ggcacgtcgt 
     1921 aggggtacat ggtggcggcc gctggcacgg ctccggcctc ggcttcgtct tcgacgagag 
     1981 tctcgacctc gatctccgtt ccgccgatgt tcagtttggt ggttttgcgg atatctccgt 
     2041 gaatcaatat gtgctcgtgg atcaggacgc gcaggtagtg tcccaacaac ccccaacaat 
     2101 ttttactcat cagatcaata atcgtgtgac ctacggtgga gccatagacg ctcaggacgc 
     2161 caaaaccttc gggccccaaa atgaagagaa ttgggaacat accccagctc acgaaaaaca 
     2221 gccatgccat gccggtcacg acctggcggc accgaccctt tggcacagta tgataaccct 
     2281 cgatatatgc tttggcggcg tgaaaaaatg tgttcgcgcc atagcacaat ccaagacaaa 
     2341 agaagatgac tttaacatag ccggttgcca tggcgctggt agccccccac acgatagtcc 
     2401 cgatgtctga gacaaggagt cccatggttc tcctgctgta gtcgttgctc aggccggtga 
     2461 ggttgctcag gcggataagg atgacaggac aagtgagcag ccaggttgca tagcgcagcc 
     2521 actgcacccg gtgtcctgtg gcaaggtaga gcatagaggg attcttaaac tcaaaaaaga 
     2581 actcgagaat caccttaacc atttcaatgg cgcacacata gatctcctcc cagccgcatg 
     2641 tagatttcca ggtttggtag gcatagaaca tcagcagcaa aatgctgaat cctgctgcaa 
     2701 gccactgcag gacatttgac gcggtctgag cgccgttcgt gccgcgagat tcaatccatc 
     2761 cggcacagta acattgatcc tcagggacca ggacggaccc gttcaccacc acaggattag 
     2821 taacgaacaa aagttcgcgt ccgacggcag acaaagcgcc gccatagtcc atggtggcta 
     2881 gcataacttc gtataaagta tcctatacga agttatttgc cttaacccag aaattatcac 
     2941 tgttattctt tagaatggtg caaagaataa cttcgtataa tgtatgctat acgaagttat 
     3001 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg 
     3061 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 
     3121 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 
     3181 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 
     3241 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 
     3301 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 
     3361 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa 
     3421 tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc 
     3481 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 
     3541 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 
     3601 gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg catccctgtg 
     3661 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg 
     3721 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg 
     3781 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg 
     3841 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg 
     3901 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat 
     3961 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg 
     4021 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc 
     4081 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca 
     4141 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg 
     4201 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg 
     4261 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt 
     4321 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg 
     4381 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 
     4441 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 
     4501 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 
     4561 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg 
     4621 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 
     4681 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg 
     4741 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 
     4801 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct 
     4861 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg 
     4921 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 
     4981 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc 
     5041 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 
     5101 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 
     5161 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 
     5221 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 
     5281 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 
     5341 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 
     5401 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 
     5461 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 
     5521 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 
     5581 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 
     5641 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 
     5701 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 
     5761 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 
     5821 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 
     5881 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 
     5941 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 
     6001 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 
     6061 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 
     6121 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
     6181 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     6241 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     6301 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     6361 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 
     6421 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     6481 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     6541 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     6601 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 
     6661 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     6721 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     6781 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     6841 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatg 
//
