LOCUS       S14-pAAV-hSynDIO        6868 bp    DNA     circular     10-JUL-2015
SOURCE      
  ORGANISM  
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            http://www.invitrogen.com/
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FEATURES             Location/Qualifiers
     LTR             1..141
                     /vntifkey="19"
                     /label=L-ITR
     misc_feature    complement(1900..2610)
                     /vntifkey="21"
                     /label=mRuby2
     LTR             4130..4270
                     /vntifkey="19"
                     /label=R-ITR
     misc_feature    complement(2623..3552)
                     /vntifkey="21"
                     /label=Cheta
     misc_feature    3563..3596
                     /vntifkey="21"
                     /label=lox2722
     misc_feature    3647..3680
                     /vntifkey="21"
                     /label=loxP
     polyA_signal    3695..4121
                     /vntifkey="25"
                     /label=W3SL
     promoter        156..640
                     /vntifkey="29"
                     /label=hSynapsin\promoter
     misc_signal     1856..1885
                     /vntifkey="87"
                     /label=myc
     primer          1900..1954
                     /vntifkey="27"
                     /label=P26:mRuby2-F
     misc_feature    complement(1856..1924)
                     /vntifkey="21"
                     /label=P27:Myc-PacI-mRuby-R
     primer          complement(3540..3566)
                     /vntifkey="27"
                     /label=AGV331
     primer_bind     150..175
                     /vntifkey="28"
                     /label=P13:MluI-hSyn-F
     misc_feature    800..1855
                     /vntifkey="21"
                     /label=mCARv2
BASE COUNT     1656 a      1796 c      1732 g      1684 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc 
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg 
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta 
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca 
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa 
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc 
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac 
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc 
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cctctagagt 
      661 cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg 
      721 attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac 
      781 gaagttatgg cgcgccacca tggctcgcct actgtgcttc gtgctcttgt gcgggatcgc 
      841 ggatttcacc agtggtttga gcatcactac acccgaacag aggatcgaaa aagccaaagg 
      901 ggaaactgcg tatctaccat gcaagtttac tctcagtccc gaagaccagg gaccactgga 
      961 cattgaatgg ctgatatccc cgtctgataa ccagatagtg gatcaagtga tcattttgta 
     1021 ttctggagac aaaatttatg ataactacta tccggatctg aaaggacggg tacattttac 
     1081 gagtaacgat gtcaagtctg gcgacgcatc tataaatgtg accaacctgc agctgtcgga 
     1141 cattggcact taccagtgca aagtgaagaa agcccctggg gttgcaaata agaaattcct 
     1201 gctgaccgtt cttgttaagc cttcaggtac aagatgcttc gtggatggat cggaagagat 
     1261 tggaaatgac ttcaagctaa aatgtgaacc caaggaaggc tcccttccac tacagtttga 
     1321 atggcagaaa ctgtcggact cccagacaat gcctacgcca tggctggcag aaatgacgtc 
     1381 accagttata tctgtgaaga acgccagttc tgagtattct gggacataca gctgcacggt 
     1441 tcaaaacaga gtgggctctg accagtgtat gctgcgacta gacgttgtcc caccctccaa 
     1501 ccgagccgga acgatcgcgg gcgccgtcat agggacgctg ctggcccttg tgctcatcgg 
     1561 ggccatcctc ttctgctgtc acaggaaacg cagagaagag aagtacgaga aggaagttca 
     1621 tcatgatatc agggaagatg tgcctcctcc aaagagtcgg acatccactg ccaggagcta 
     1681 tattggcagc aaccattcct ccctgggatc catgtccccc tctaacatgg aggggtattc 
     1741 caagacgcag tataaccaag tccccagtga agactttgaa cgtgcgcctc agagcccgac 
     1801 tctggcaccc gctaagttca agtacgctta caagaccgat ggcattacag tggtagagca 
     1861 gaagctgatc agcgaggagg acctgtaatt aattaattac ttgtacagct cgtccatccc 
     1921 accaccaagc ccggcgaact tggcaactgc gtgttcgcgt tgtactacga acatttcatt 
     1981 gtcactttcc tctaaccttt ccaggcggtg atcaacggca tggataccgg gcatcttgat 
     2041 gttcccgacg gtcttttttg acctgtaagt tgttacgaaa gagcaagaca gatggccacc 
     2101 accatcaact ttcagtgcca tatgagtgta tcccctcaga ccaccatctg ctggatacat 
     2161 catctctgta ttaggctccc aacccttggt cttcttctgc atcacgggac cattggaggg 
     2221 aaagtttacc cctctgactt ggacgtggta aacgagacag ccatcctcaa ggctggtgtc 
     2281 ctgcatgacg gtgacgactc caccatcttc gtatctcgta actctttccc aagtaaaacc 
     2341 ctcaggaaag gactgtttaa agaaatcagg aatgcctttc gggtacttga taaaagtacg 
     2401 gctgccatac atgaacgacg tggcaagaat gtcaaaggca aatggcaggg gtcctccctc 
     2461 gatgactttg atcctcatgg tttgagttcc catgtacgga ttgccttctc cttcacctgt 
     2521 gcatttgaat tggtggccgt tgaccgaacc ttccatgacc accttcatac gcatattttc 
     2581 cttgatcagc tcttcgccct tagacaccat ggtggcggcc gctggcacgg ctccggcctc 
     2641 ggcttcgtct tcgacgagag tctcgacctc gatctccgtt ccgccgatgt tcagtttggt 
     2701 ggttttgcgg atatctccgt gaatcaatat gtgctcgtgg atcaggacgc gcaggtagtg 
     2761 tcccaacaac ccccaacaat ttttactcat cagatcaata atcgtgtgac ctacggtgga 
     2821 gccatagacg ctcaggacgc caaaaccttc gggccccaaa atgaagagaa ttgggaacat 
     2881 accccagctc acgaaaaaca gccatgccat gccggtcacg acctggcggc accgaccctt 
     2941 tggcacagta tgataaccct cgatatatgc tttggcggcg tgaaaaaatg tgttcgcgcc 
     3001 atagcacaat ccaagacaaa agaagatgac tttaacatag ccggttgcca tggcgctggt 
     3061 agccccccac acgatagtcc cgatgtctga gacaaggagt cccatggttc tcctgctgta 
     3121 gtcgttgctc aggccggtga ggttgctcag gcggataagg atgacaggac aagtgagcag 
     3181 ccaggttgca tagcgcagcc actgcacccg gtgtcctgtg gcaaggtaga gcatagaggg 
     3241 attcttaaac tcaaaaaaga actcgagaat caccttaacc atttcaatgg cgcacacata 
     3301 gatctcctcc cagccgcatg tagatttcca ggtttggtag gcatagaaca tcagcagcaa 
     3361 aatgctgaat cctgctgcaa gccactgcag gacatttgac gcggtctgag cgccgttcgt 
     3421 gccgcgagat tcaatccatc cggcacagta acattgatcc tcagggacca ggacggaccc 
     3481 gttcaccacc acaggattag taacgaacaa aagttcgcgt ccgacggcag acaaagcgcc 
     3541 gccatagtcc atggtggcta gcataacttc gtataaagta tcctatacga agttatttgc 
     3601 cttaacccag aaattatcac tgttattctt tagaatggtg caaagaataa cttcgtataa 
     3661 tgtatgctat acgaagttat gaattccgct cgagataatc aacctctgga ttacaaaatt 
     3721 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 
     3781 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 
     3841 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 
     3901 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt 
     3961 gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc 
     4021 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt 
     4081 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcggccgca ggaaccccta 
     4141 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca 
     4201 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc 
     4261 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac 
     4321 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg 
     4381 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 
     4441 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 
     4501 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 
     4561 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 
     4621 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta 
     4681 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa 
     4741 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt 
     4801 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc 
     4861 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 
     4921 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 
     4981 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 
     5041 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 
     5101 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 
     5161 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 
     5221 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 
     5281 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 
     5341 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 
     5401 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 
     5461 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 
     5521 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 
     5581 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 
     5641 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 
     5701 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 
     5761 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 
     5821 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 
     5881 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 
     5941 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 
     6001 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 
     6061 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 
     6121 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 
     6181 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 
     6241 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 
     6301 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 
     6361 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 
     6421 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 
     6481 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 
     6541 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 
     6601 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 
     6661 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 
     6721 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 
     6781 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 
     6841 ccttttgctg gccttttgct cacatgtt 
//
