LOCUS       S23-pAAV-hSynDIO        6913 bp    DNA     circular     18-JAN-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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FEATURES             Location/Qualifiers
     LTR             1..141
                     /vntifkey="19"
                     /label=L-ITR
     LTR             4175..4315
                     /vntifkey="19"
                     /label=R-ITR
     misc_feature    3608..3641
                     /vntifkey="21"
                     /label=lox2722
     misc_feature    3692..3725
                     /vntifkey="21"
                     /label=loxP
     polyA_signal    3740..4166
                     /vntifkey="25"
                     /label=W3SL
     promoter        156..640
                     /vntifkey="29"
                     /label=hSynapsin\promoter
     CDS             800..1894
                     /vntifkey="4"
                     /label=hCAR
     misc_signal     1895..1924
                     /vntifkey="87"
                     /label=myc
     primer_bind     150..175
                     /vntifkey="28"
                     /label=P13:MluI-hSyn-F
     misc_feature    complement(1939..3597)
                     /vntifkey="21"
                     /label=ChETA-EYFP
BASE COUNT     1635 a      1762 c      1803 g      1713 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc 
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg 
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta 
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca 
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa 
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc 
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac 
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc 
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cctctagagt 
      661 cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg 
      721 attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac 
      781 gaagttatgg cgcgccacca tggcgctcct gctgtgcttc gtgctcctgt gcggagtagt 
      841 ggatttcgcc agaagtttga gtatcactac tcctgaagag atgattgaaa aagccaaagg 
      901 ggaaactgcc tatctgccgt gcaaatttac gcttagtccc gaagaccagg gaccgctgga 
      961 catcgagtgg ctgatatcac cagctgataa tcagaaggtg gatcaagtga ttattttata 
     1021 ttctggagac aaaatttatg atgactacta tccagatctg aaaggccgag tacattttac 
     1081 gagtaatgat ctcaaatctg gtgatgcatc aataaatgta acgaatttac aactgtcaga 
     1141 tattggcaca tatcagtgca aagtgaaaaa agctcctggt gttgcaaata agaagattca 
     1201 tctggtagtt cttgttaagc cttcaggtgc gagatgttac gttgatggat ctgaagaaat 
     1261 tggaagtgac tttaagataa aatgtgaacc aaaagaaggt tcacttccat tacagtatga 
     1321 gtggcaaaaa ttgtctgact cacagaaaat gcccacttca tggttagcag aaatgacttc 
     1381 atctgttata tctgtaaaaa atgcctcttc tgagtactct gggacataca gctgtacagt 
     1441 cagaaacaga gtgggctctg atcagtgcct gttgcgtcta aacgttgtcc ctccttcaaa 
     1501 taaagctgga ctaattgcag gagccattat aggaactttg cttgctctag cgctcattgg 
     1561 tcttatcatc ttttgctgtc gtaaaaagcg cagagaagaa aaatatgaaa aggaagttca 
     1621 tcacgatatc agggaagatg tgccacctcc aaagagccgt acgtccactg ccagaagcta 
     1681 catcggcagt aatcattcat ccctggggtc catgtctcct tccaacatgg aaggatattc 
     1741 caagactcag tataaccaag taccaagtga agactttgaa cgcactcctc agagtccgac 
     1801 tctcccacct gctaaggtag ctgcccctaa tctaagtcga atgggtgcga ttcctgtgat 
     1861 gattccagca cagagcaagg atgggtctat agtagagcag aagctgatca gcgaggagga 
     1921 cctgtaatta attaattact tgtacagctc gtccatgccg agagtgatcc cggcggcggt 
     1981 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga 
     2041 ctggtagctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt 
     2101 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa 
     2161 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta 
     2221 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag 
     2281 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt 
     2341 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt 
     2401 gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg aagcactgca ggccgtagcc 
     2461 gaaggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt 
     2521 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg 
     2581 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc 
     2641 gcccttgctc accatggtgg cggccgctgg cacggctccg gcctcggctt cgtcttcgac 
     2701 gagagtctcg acctcgatct ccgttccgcc gatgttcagt ttggtggttt tgcggatatc 
     2761 tccgtgaatc aatatgtgct cgtggatcag gacgcgcagg tagtgtccca acaaccccca 
     2821 acaattttta ctcatcagat caataatcgt gtgacctacg gtggagccat agacgctcag 
     2881 gacgccaaaa ccttcgggcc ccaaaatgaa gagaattggg aacatacccc agctcacgaa 
     2941 aaacagccat gccatgccgg tcacgacctg gcggcaccga ccctttggca cagtatgata 
     3001 accctcgata tatgctttgg cggcgtgaaa aaatgtgttc gcgccatagc acaatccaag 
     3061 acaaaagaag atgactttaa catagccggt tgccatggcg ctggtagccc cccacacgat 
     3121 agtcccgatg tctgagacaa ggagtcccat ggttctcctg ctgtagtcgt tgctcaggcc 
     3181 ggtgaggttg ctcaggcgga taaggatgac aggacaagtg agcagccagg ttgcatagcg 
     3241 cagccactgc acccggtgtc ctgtggcaag gtagagcata gagggattct taaactcaaa 
     3301 aaagaactcg agaatcacct taaccatttc aatggcgcac acatagatct cctcccagcc 
     3361 gcatgtagat ttccaggttt ggtaggcata gaacatcagc agcaaaatgc tgaatcctgc 
     3421 tgcaagccac tgcaggacat ttgacgcggt ctgagcgccg ttcgtgccgc gagattcaat 
     3481 ccatccggca cagtaacatt gatcctcagg gaccaggacg gacccgttca ccaccacagg 
     3541 attagtaacg aacaaaagtt cgcgtccgac ggcagacaaa gcgccgccat agtccatggt 
     3601 ggctagcata acttcgtata aagtatccta tacgaagtta tttgccttaa cccagaaatt 
     3661 atcactgtta ttctttagaa tggtgcaaag aataacttcg tataatgtat gctatacgaa 
     3721 gttatgaatt ccgctcgaga taatcaacct ctggattaca aaatttgtga aagattgact 
     3781 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 
     3841 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta 
     3901 gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 
     3961 ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta 
     4021 tttgtaacca tctagcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat 
     4081 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca 
     4141 gggggagatg tgggaggttt tttaaagcgg ccgcaggaac ccctagtgat ggagttggcc 
     4201 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc 
     4261 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc 
     4321 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc 
     4381 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 
     4441 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 
     4501 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 
     4561 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac 
     4621 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 
     4681 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt 
     4741 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac 
     4801 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca 
     4861 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg 
     4921 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct 
     4981 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg 
     5041 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 
     5101 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 
     5161 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 
     5221 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 
     5281 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 
     5341 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 
     5401 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 
     5461 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 
     5521 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 
     5581 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 
     5641 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 
     5701 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 
     5761 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 
     5821 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 
     5881 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 
     5941 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 
     6001 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 
     6061 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 
     6121 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
     6181 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     6241 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     6301 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     6361 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 
     6421 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     6481 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     6541 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     6601 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     6661 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     6721 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     6781 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     6841 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     6901 ttgctcacat gtt 
//
