LOCUS       S18-pAAV-hSynDIO        7286 bp    DNA     circular      1-JUN-2016
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-11263428|
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COMMENT                             "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                                            " N2: 2797 " 1))))) "KpnI: 2 sites") 
COMMENT                            5 1 "GGTACC" "CCATGG") 
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COMMENT                            5 "ACGCGT" "TGCGCA") 
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COMMENT                            1 5 "CCATGG" "GGTACC") 
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COMMENT                            1 5 "GCTAGC" "CGATCG") 
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COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                            5 3 "TTAATTAA" "AATTAATT") 
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COMMENT                            3 3 "CACGTG" "GTGCAC") 
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COMMENT                            5 1 "CTGCAG" "GACGTC") 
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COMMENT                            4 2 "CGATCG" "GCTAGC") 
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COMMENT                            3 3 "CAGCTG" "GTCGAC") 
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COMMENT                            5 1 "GAGCTC" "CTCGAG") 
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COMMENT                            4 2 "CCGCGG" "GGCGCC") 
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COMMENT                            1 5 "GTCGAC" "CAGCTG") 
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COMMENT                            3 3 "CCCGGG" "GGGCCC") 
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COMMENT                            1 5 "CTCGAG" "GAGCTC") 
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COMMENT                            1 5 "CCCGGG" "GGGCCC"))) 
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FEATURES             Location/Qualifiers
     misc_feature    1887..1889
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
                     /vntifkey="21"
                     /label=TAA
     misc_feature    756..789
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=lox
     misc_feature    1857..1886
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
                     /vntifkey="21"
                     /label=MYC
     misc_feature    complement(3356..3389)
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=loxP
     misc_feature    1890..1897
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
                     /vntifkey="21"
                     /label=PacI
     misc_feature    795..800
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
                     /vntifkey="21"
                     /label=Kozak
     misc_feature    5356..5378
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="21"
                     /label=pGEX_3_primer
     rep_origin      4782..5088
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="33"
                     /label=f1_origin
     CDS             5607..6467
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="4"
                     /label=ORF\frame\1
     misc_feature    671..704
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=Lox
     rep_origin      6622..7241
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="33"
                     /label=pBR322_origin
     terminator      4032..4510
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="43"
                     /label=hGH_PA_terminator
     misc_feature    790..797
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
                     /vntifkey="21"
                     /label=AscI
     promoter        5537..5565
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    complement(3272..3305)
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=loxP
     misc_feature    3416..4003
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    157..641
                     /vntifkey="21"
                     /label=hSynapsin\promoter
     misc_feature    801..1856
                     /vntifkey="21"
                     /label=mCARv2
     primer          785..823
                     /vntifkey="27"
                     /label=AscI-mCar-F
     misc_feature    complement(1901..3271)
                     /vntifkey="21"
                     /label=GCamp6f
     LTR             4550..4690
                     /vntifkey="19"
                     /label=R-ITR
     LTR             2..142
                     /vntifkey="19"
                     /label=L-ITR
BASE COUNT     1570 a      1990 c      1896 g      1830 t 
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt 
       61 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc 
      121 aactccatca ctaggggttc ctgcggccgc acgcgtgtgt ctagactgca gagggccctg 
      181 cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac 
      241 gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct 
      301 atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc 
      361 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga 
      421 aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg 
      481 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca 
      541 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg 
      601 cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaga aggtaccgga tcctctagag 
      661 tcgactccgg aataacttcg tataggatac tttatacgaa gttatgcaga atggtagctg 
      721 gattgtagct gctattagca atatgaaacc tcttaataac ttcgtatagc atacattata 
      781 cgaagttatg gcgcgccacc atggctcgcc tactgtgctt cgtgctcttg tgcgggatcg 
      841 cggatttcac cagtggtttg agcatcacta cacccgaaca gaggatcgaa aaagccaaag 
      901 gggaaactgc gtatctacca tgcaagttta ctctcagtcc cgaagaccag ggaccactgg 
      961 acattgaatg gctgatatcc ccgtctgata accagatagt ggatcaagtg atcattttgt 
     1021 attctggaga caaaatttat gataactact atccggatct gaaaggacgg gtacatttta 
     1081 cgagtaacga tgtcaagtct ggcgacgcat ctataaatgt gaccaacctg cagctgtcgg 
     1141 acattggcac ttaccagtgc aaagtgaaga aagcccctgg ggttgcaaat aagaaattcc 
     1201 tgctgaccgt tcttgttaag ccttcaggta caagatgctt cgtggatgga tcggaagaga 
     1261 ttggaaatga cttcaagcta aaatgtgaac ccaaggaagg ctcccttcca ctacagtttg 
     1321 aatggcagaa actgtcggac tcccagacaa tgcctacgcc atggctggca gaaatgacgt 
     1381 caccagttat atctgtgaag aacgccagtt ctgagtattc tgggacatac agctgcacgg 
     1441 ttcaaaacag agtgggctct gaccagtgta tgctgcgact agacgttgtc ccaccctcca 
     1501 accgagccgg aacgatcgcg ggcgccgtca tagggacgct gctggccctt gtgctcatcg 
     1561 gggccatcct cttctgctgt cacaggaaac gcagagaaga gaagtacgag aaggaagttc 
     1621 atcatgatat cagggaagat gtgcctcctc caaagagtcg gacatccact gccaggagct 
     1681 atattggcag caaccattcc tccctgggat ccatgtcccc ctctaacatg gaggggtatt 
     1741 ccaagacgca gtataaccaa gtccccagtg aagactttga acgtgcgcct cagagcccga 
     1801 ctctggcacc cgctaagttc aagtacgctt acaagaccga tggcattaca gtggtagagc 
     1861 agaagctgat cagcgaggag gacctgtaat taattaatta cttcgctgtc atcatttgta 
     1921 caaactcttc gtagtttacc tgaccatccc catcgatgtc tgcttccctg atcatttcat 
     1981 caacctcttc atctgttaac ttctctccaa ggtttgtcat cacgtggcga agctctgctg 
     2041 cactgatgta gccattgcca tccttatcaa acacaccgaa cgcttctcta atttcttctt 
     2101 ccgtgtccct gtatttcatt tttcttgcca tcattgtcag gaactcaggg aagtcgattg 
     2161 tgccgtcacc gtcggcatct acttcattga tcatgtcctg cagctctgct tctgtggggt 
     2221 tctgccccag agaccgcatc accgtcccca gctccttggt tgttattgtc ccatccccgt 
     2281 ccttgtcaaa tagggagaat tcctctttaa attctgcgat ctgctcttca gtcagttggt 
     2341 ccggcaggtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc 
     2401 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct 
     2461 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gatgtagcct tcgggcatgg 
     2521 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc 
     2581 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga 
     2641 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc accctcgccg gacacgctga 
     2701 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca 
     2761 gctcctcgcc cttgctcacc atgctccctc cggtaccgcc cttgtacagc tcgtccatgc 
     2821 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt 
     2881 tggggtcttt cgaaagtttg gactgcacgc tcaggtagtg gttgtcgggc agcagcacgg 
     2941 ggccgtcgcc gatgggggtg ttctgctggt agtggtaggc gagctgcacg ccgccgtcct 
     3001 cgatgttgtg gcggatcttg aagttcgcct tgatgccgtt cttctgcttg tcggccttga 
     3061 tatagacgtt ctcgagtgag ctcagccgac ctatagctct gactgcgtga cctgtcttat 
     3121 tccacttacg acgtgatgag tcgaccatgg tggcgagatc cttatcgtca tcgtcgtaca 
     3181 gatcccgacc catttgctgt ccaccagtca tgctagccat accatgatga tgatgatgat 
     3241 gagaacccat ggtggcaaca gcgctactag cataacttcg tataaagtat cctatacgaa 
     3301 gttatttgcc ttaacccaga aattatcact gttattcttt agaatggtgc aaagaataac 
     3361 ttcgtataat gtatgctata cgaagttatg aattcgatat caagcttatc gataatcaac 
     3421 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 
     3481 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 
     3541 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 
     3601 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 
     3661 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 
     3721 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 
     3781 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg 
     3841 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 
     3901 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 
     3961 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc 
     4021 tcgagagatc tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga 
     4081 agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc 
     4141 tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca 
     4201 agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt 
     4261 ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca 
     4321 gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt 
     4381 ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt 
     4441 gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt 
     4501 ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta 
     4561 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca 
     4621 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc 
     4681 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac 
     4741 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg 
     4801 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 
     4861 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 
     4921 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 
     4981 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 
     5041 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 
     5101 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 
     5161 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt 
     5221 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc 
     5281 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 
     5341 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 
     5401 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 
     5461 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 
     5521 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 
     5581 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 
     5641 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 
     5701 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 
     5761 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 
     5821 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 
     5881 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 
     5941 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 
     6001 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 
     6061 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 
     6121 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 
     6181 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 
     6241 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 
     6301 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 
     6361 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 
     6421 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 
     6481 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 
     6541 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 
     6601 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 
     6661 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 
     6721 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 
     6781 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 
     6841 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 
     6901 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 
     6961 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 
     7021 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 
     7081 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 
     7141 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 
     7201 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 
     7261 ccttttgctg gccttttgct cacatg 
//
