LOCUS       S20-pAAV-hSynDIO        7287 bp    DNA     circular      1-JUN-2016
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-11263435|
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COMMENT                            5 1 "CTGCAG" "GACGTC") 
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COMMENT                            3 3 "CCCGGG" "GGGCCC") 
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FEATURES             Location/Qualifiers
     misc_feature    1887..1889
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
                     /vntifkey="21"
                     /label=TAA
     misc_feature    756..789
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=lox
     misc_feature    1857..1886
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
                     /vntifkey="21"
                     /label=MYC
     misc_feature    complement(3357..3390)
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=loxP
     misc_feature    1890..1897
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
                     /vntifkey="21"
                     /label=PacI
     misc_feature    795..800
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
                     /vntifkey="21"
                     /label=Kozak
     misc_feature    5357..5379
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="21"
                     /label=pGEX_3_primer
     rep_origin      4783..5089
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="33"
                     /label=f1_origin
     CDS             5608..6468
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="4"
                     /label=ORF\frame\1
     misc_feature    671..704
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=Lox
     rep_origin      6623..7242
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="33"
                     /label=pBR322_origin
     terminator      4033..4511
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="43"
                     /label=hGH_PA_terminator
     misc_feature    790..797
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
                     /vntifkey="21"
                     /label=AscI
     promoter        5538..5566
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    complement(3273..3306)
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
                     /vntifkey="21"
                     /label=loxP
     misc_feature    3417..4004
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    157..641
                     /vntifkey="21"
                     /label=hSynapsin\promoter
     misc_feature    801..1856
                     /vntifkey="21"
                     /label=mCARv2
     primer          785..823
                     /vntifkey="27"
                     /label=AscI-mCar-F
     LTR             4551..4691
                     /vntifkey="19"
                     /label=R-ITR
     LTR             2..142
                     /vntifkey="19"
                     /label=L-ITR
     misc_feature    complement(1901..3262)
                     /vntifkey="21"
                     /label=DTR-GFP
BASE COUNT     1574 a      1985 c      1940 g      1788 t 
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt 
       61 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc 
      121 aactccatca ctaggggttc ctgcggccgc acgcgtgtgt ctagactgca gagggccctg 
      181 cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac 
      241 gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct 
      301 atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc 
      361 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga 
      421 aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg 
      481 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca 
      541 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg 
      601 cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaga aggtaccgga tcctctagag 
      661 tcgactccgg aataacttcg tataggatac tttatacgaa gttatgcaga atggtagctg 
      721 gattgtagct gctattagca atatgaaacc tcttaataac ttcgtatagc atacattata 
      781 cgaagttatg gcgcgccacc atggctcgcc tactgtgctt cgtgctcttg tgcgggatcg 
      841 cggatttcac cagtggtttg agcatcacta cacccgaaca gaggatcgaa aaagccaaag 
      901 gggaaactgc gtatctacca tgcaagttta ctctcagtcc cgaagaccag ggaccactgg 
      961 acattgaatg gctgatatcc ccgtctgata accagatagt ggatcaagtg atcattttgt 
     1021 attctggaga caaaatttat gataactact atccggatct gaaaggacgg gtacatttta 
     1081 cgagtaacga tgtcaagtct ggcgacgcat ctataaatgt gaccaacctg cagctgtcgg 
     1141 acattggcac ttaccagtgc aaagtgaaga aagcccctgg ggttgcaaat aagaaattcc 
     1201 tgctgaccgt tcttgttaag ccttcaggta caagatgctt cgtggatgga tcggaagaga 
     1261 ttggaaatga cttcaagcta aaatgtgaac ccaaggaagg ctcccttcca ctacagtttg 
     1321 aatggcagaa actgtcggac tcccagacaa tgcctacgcc atggctggca gaaatgacgt 
     1381 caccagttat atctgtgaag aacgccagtt ctgagtattc tgggacatac agctgcacgg 
     1441 ttcaaaacag agtgggctct gaccagtgta tgctgcgact agacgttgtc ccaccctcca 
     1501 accgagccgg aacgatcgcg ggcgccgtca tagggacgct gctggccctt gtgctcatcg 
     1561 gggccatcct cttctgctgt cacaggaaac gcagagaaga gaagtacgag aaggaagttc 
     1621 atcatgatat cagggaagat gtgcctcctc caaagagtcg gacatccact gccaggagct 
     1681 atattggcag caaccattcc tccctgggat ccatgtcccc ctctaacatg gaggggtatt 
     1741 ccaagacgca gtataaccaa gtccccagtg aagactttga acgtgcgcct cagagcccga 
     1801 ctctggcacc cgctaagttc aagtacgctt acaagaccga tggcattaca gtggtagagc 
     1861 agaagctgat cagcgaggag gacctgtaat taattaatta cttgtacagc tcgtccatgc 
     1921 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt 
     1981 tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg 
     2041 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct 
     2101 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga 
     2161 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct 
     2221 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca 
     2281 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt 
     2341 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc 
     2401 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct 
     2461 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct 
     2521 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca 
     2581 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatccgggt 
     2641 gggaattagt catgcccaac ttcactttct cttcgttttc cacatcataa ccacctctcc 
     2701 tatggtacct aaacatgaga agccccacga tgaccagcag acagacagag gacagcacca 
     2761 cggccaccac agccaggata gttgtatggt cataggtata taagcgattt tccactggga 
     2821 ggctcagccc atgacacctc tctccatggt aacctgggtg gcagatgcag gagggagccc 
     2881 ggagctcctt cacatatttg cattctccgt ggatgcagaa gtccttgtat ttccgaagac 
     2941 atgggtccct cttcttccct agtcccttgc ctttcttctt tcttttcccg tgctcctcct 
     3001 tgcttggtgt ggccagtgct tgtggcttgg aggataaagt gactctcaaa aggtccagat 
     3061 ctgcctcttg caagtcacgg actttccggt cccggccgcc tcctaggcgt agcagctggt 
     3121 ccgtagatcc agtggaaggg tccgggttgc tggttccagc agctagccct ctccgaagct 
     3181 gctccaggct ctcgccagtc accagtgccg aaagaactgc agccagaagg agcttcagca 
     3241 ccaccgacgg cagcagcttc atggtgacta gcataacttc gtataaagta tcctatacga 
     3301 agttatttgc cttaacccag aaattatcac tgttattctt tagaatggtg caaagaataa 
     3361 cttcgtataa tgtatgctat acgaagttat gaattcgata tcaagcttat cgataatcaa 
     3421 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 
     3481 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 
     3541 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 
     3601 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 
     3661 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 
     3721 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 
     3781 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat 
     3841 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 
     3901 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 
     3961 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg 
     4021 ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg 
     4081 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt 
     4141 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc 
     4201 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag 
     4261 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc 
     4321 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt 
     4381 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg 
     4441 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct 
     4501 tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct 
     4561 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc 
     4621 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag 
     4681 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 
     4741 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt 
     4801 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 
     4861 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 
     4921 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 
     4981 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 
     5041 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 
     5101 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 
     5161 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 
     5221 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac 
     5281 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 
     5341 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 
     5401 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 
     5461 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 
     5521 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 
     5581 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 
     5641 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 
     5701 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 
     5761 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 
     5821 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 
     5881 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 
     5941 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 
     6001 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 
     6061 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 
     6121 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 
     6181 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 
     6241 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 
     6301 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 
     6361 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 
     6421 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 
     6481 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 
     6541 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 
     6601 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
     6661 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 
     6721 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 
     6781 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 
     6841 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 
     6901 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 
     6961 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 
     7021 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 
     7081 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 
     7141 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 
     7201 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 
     7261 gccttttgct ggccttttgc tcacatg 
//
