LOCUS       S21-pAAV-hCARmyc        6616 bp    DNA     circular     13-JUN-2016
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-11280591|
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FEATURES             Location/Qualifiers
     LTR             1..141
                     /vntifkey="19"
                     /label=L-ITR
     LTR             3878..4018
                     /vntifkey="19"
                     /label=R-ITR
     misc_feature    3311..3344
                     /vntifkey="21"
                     /label=lox2722
     misc_feature    3395..3428
                     /vntifkey="21"
                     /label=loxP
     polyA_signal    3443..3869
                     /vntifkey="25"
                     /label=W3SL
     promoter        156..640
                     /vntifkey="29"
                     /label=hSynapsin\promoter
     CDS             800..1894
                     /vntifkey="4"
                     /label=hCAR
     misc_signal     1895..1924
                     /vntifkey="87"
                     /label=myc
     primer_bind     150..175
                     /vntifkey="28"
                     /label=P13:MluI-hSyn-F
     misc_feature    complement(1936..3300)
                     /vntifkey="21"
                     /label=DTR-GFP
     primer          1928..1960
                     /vntifkey="27"
                     /label=P39:\PacI-GFPr-F
     primer          complement(3281..3309)
                     /vntifkey="27"
                     /label=P40:\SpeI-DTRr-R
BASE COUNT     1523 a      1692 c      1724 g      1677 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc 
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg 
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta 
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca 
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa 
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc 
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac 
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc 
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cctctagagt 
      661 cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg 
      721 attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac 
      781 gaagttatgg cgcgccacca tggcgctcct gctgtgcttc gtgctcctgt gcggagtagt 
      841 ggatttcgcc agaagtttga gtatcactac tcctgaagag atgattgaaa aagccaaagg 
      901 ggaaactgcc tatctgccgt gcaaatttac gcttagtccc gaagaccagg gaccgctgga 
      961 catcgagtgg ctgatatcac cagctgataa tcagaaggtg gatcaagtga ttattttata 
     1021 ttctggagac aaaatttatg atgactacta tccagatctg aaaggccgag tacattttac 
     1081 gagtaatgat ctcaaatctg gtgatgcatc aataaatgta acgaatttac aactgtcaga 
     1141 tattggcaca tatcagtgca aagtgaaaaa agctcctggt gttgcaaata agaagattca 
     1201 tctggtagtt cttgttaagc cttcaggtgc gagatgttac gttgatggat ctgaagaaat 
     1261 tggaagtgac tttaagataa aatgtgaacc aaaagaaggt tcacttccat tacagtatga 
     1321 gtggcaaaaa ttgtctgact cacagaaaat gcccacttca tggttagcag aaatgacttc 
     1381 atctgttata tctgtaaaaa atgcctcttc tgagtactct gggacataca gctgtacagt 
     1441 cagaaacaga gtgggctctg atcagtgcct gttgcgtcta aacgttgtcc ctccttcaaa 
     1501 taaagctgga ctaattgcag gagccattat aggaactttg cttgctctag cgctcattgg 
     1561 tcttatcatc ttttgctgtc gtaaaaagcg cagagaagaa aaatatgaaa aggaagttca 
     1621 tcacgatatc agggaagatg tgccacctcc aaagagccgt acgtccactg ccagaagcta 
     1681 catcggcagt aatcattcat ccctggggtc catgtctcct tccaacatgg aaggatattc 
     1741 caagactcag tataaccaag taccaagtga agactttgaa cgcactcctc agagtccgac 
     1801 tctcccacct gctaaggtag ctgcccctaa tctaagtcga atgggtgcga ttcctgtgat 
     1861 gattccagca cagagcaagg atgggtctat agtagagcag aagctgatca gcgaggagga 
     1921 cctgtaatta attaattact tgtacagctc gtccatgccg agagtgatcc cggcggcggt 
     1981 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga 
     2041 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt 
     2101 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa 
     2161 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta 
     2221 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag 
     2281 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt 
     2341 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt 
     2401 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt 
     2461 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt 
     2521 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg 
     2581 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc 
     2641 gcccttgctc accatggtgg cgaccggtgg atccgggtgg gaattagtca tgcccaactt 
     2701 cactttctct tcgttttcca catcataacc acctctccta tggtacctaa acatgagaag 
     2761 ccccacgatg accagcagac agacagagga cagcaccacg gccaccacag ccaggatagt 
     2821 tgtatggtca taggtatata agcgattttc cactgggagg ctcagcccat gacacctctc 
     2881 tccatggtaa cctgggtggc agatgcagga gggagcccgg agctccttca catatttgca 
     2941 ttctccgtgg atgcagaagt ccttgtattt ccgaagacat gggtccctct tcttccctag 
     3001 tcccttgcct ttcttctttc ttttcccgtg ctcctccttg cttggtgtgg ccagtgcttg 
     3061 tggcttggag gataaagtga ctctcaaaag gtccagatct gcctcttgca agtcacggac 
     3121 tttccggtcc cggccgcctc ctaggcgtag cagctggtcc gtagatccag tggaagggtc 
     3181 cgggttgctg gttccagcag ctagccctct ccgaagctgc tccaggctct cgccagtcac 
     3241 cagtgccgaa agaactgcag ccagaaggag cttcagcacc accgacggca gcagcttcat 
     3301 ggtgactagc ataacttcgt ataaagtatc ctatacgaag ttatttgcct taacccagaa 
     3361 attatcactg ttattcttta gaatggtgca aagaataact tcgtataatg tatgctatac 
     3421 gaagttatga attccgctcg agataatcaa cctctggatt acaaaatttg tgaaagattg 
     3481 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 
     3541 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 
     3601 ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 
     3661 cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga tgctattgct 
     3721 ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt tatttgtaac 
     3781 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt 
     3841 tcagggggag atgtgggagg ttttttaaag cggccgcagg aacccctagt gatggagttg 
     3901 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga 
     3961 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg 
     4021 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa 
     4081 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 
     4141 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 
     4201 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 
     4261 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt 
     4321 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 
     4381 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt 
     4441 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt 
     4501 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc 
     4561 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg 
     4621 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg 
     4681 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa 
     4741 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 
     4801 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 
     4861 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 
     4921 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 
     4981 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 
     5041 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 
     5101 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 
     5161 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 
     5221 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 
     5281 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 
     5341 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 
     5401 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 
     5461 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 
     5521 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 
     5581 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 
     5641 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 
     5701 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 
     5761 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 
     5821 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 
     5881 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 
     5941 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 
     6001 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 
     6061 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 
     6121 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 
     6181 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 
     6241 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 
     6301 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 
     6361 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 
     6421 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 
     6481 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 
     6541 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 
     6601 cttttgctca catgtt 
//
