LOCUS       Exported                4257 bp ds-DNA     circular SYN 30-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p1512-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Parsa M. Nafisi, Tural Aksel, Shawn M. Douglas
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds.
  JOURNAL   2018
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Shawn Douglas Lab
  TITLE     Direct Submission
  JOURNAL   Exported Apr 30, 2018 from SnapGene 2.6.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     rep_origin      44..424
                     /direction=RIGHT
                     /note="M13ori"
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    324..328
                     /note="ori-alpha"
     misc_feature    342..346
                     /note="ori-beta"
     misc_feature    351..356
                     /note="ori-gamma"
     misc_feature    360..365
                     /note="ori-delta"
     misc_feature    430..1549
                     /note="p1512_Insert"
     misc_feature    1555..1875
                     /note="M13term"
     misc_feature    1836..1840
                     /note="term-alpha"
     misc_feature    1854..1858
                     /note="term-beta"
     misc_feature    1863..1868
                     /note="term-gamma"
     rep_origin      complement(2293..2881)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3052..3912)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3913..4017)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc aactgctggg ccatatcgac atggacacca gagaatggag cgacggcgtg
      481 ctcacaaact ccgccagaca agtcgtgcgc gaacctcaag acgtcagctc ttggatcatc
      541 tgcgatggtg atattgaccc tgagtggatc gagtccctga attccgtgtt ggatgacaac
      601 aggctcctca caatgccttc tggtgagaga atccagttcg gtcctaacgt gaacttcgtg
      661 ttcgagacac acgatctcag ctgtgctagc ccagctacta tctcccgcat gggaatgatc
      721 ttcttgtccg acgaggagac agatttgaac tcattgatca agtcttggct cagaaaccag
      781 cctgcagaat ataggaataa cctggagaac tggatcggtg attacttcga gaaggctttg
      841 cagtgggtgc tgaaacagaa cgactatgtc gtcgaaacca gcctggtcgg tacagttatg
      901 aacggactct cccatctgca cggatgcaga gatcacgatg agtttatcat caatttgatc
      961 cgcggactgg gaggtaactt gaatatgaaa tctcgcctgg agttcactaa agaagtgttc
     1021 cactgggcta gggagtcacc acctgacttc cacaaaccta tggacaccta ctatgattcc
     1081 acaagaggca ggttggccac ctacgtgctg aagaagcctg aggacctcac cgctgacgac
     1141 ttctccaacg gactgactct gcccgtgatc cagactccag acatgcagcg cggactcgat
     1201 tactttaagc cctggctcag ctccgatacc aagcaacctt tcattctcgt gggaccagag
     1261 ggatgtggta aaggaatgct cctgaggtat gcattctccc agctccgctc aacccaaatt
     1321 gccactgttc actgttcagc ccaaacaact tcaaggcatc tcctccagaa gctcagccag
     1381 acctgtatgg ttatcagcac caacaccggc agagtttacc gcccaaagga ttgtgagcgc
     1441 ctcgtgttgt acctcaaaga tatcaatctg cccaaactcg ataagtgggg cacttccacc
     1501 ctcgtggcat ttctccaaca ggtgctgacc taccagggct tctacgacgg gatccacgcg
     1561 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1621 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1681 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1741 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     1801 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1861 ttgttccaaa ctggagaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1921 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1981 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2041 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2101 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2161 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2221 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2281 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2341 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2401 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2461 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2521 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2581 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2641 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2701 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2761 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2821 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2881 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2941 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3001 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3061 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3121 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3181 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3241 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3301 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3361 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3421 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3481 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3541 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3601 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3661 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3721 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3781 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3841 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3901 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3961 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4021 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     4081 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     4141 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     4201 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaa
//
