LOCUS       pCS6-YFP-SMASh(A        8340 bp    DNA     circular UNA 21-MAY-2018
DEFINITION  pCS6-Venus-SMASh II orthogonal variant.
ACCESSION   urn.local...91pe6j5-1a
VERSION     urn.local...91pe6j5-1a
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      86..541
                     /label="M13 origin"
     CDS             829..1689
                     /label="AmpR"
     promoter        2950..3476
                     /label="CMV promoter"
     misc_feature    3672..3677
                     /note="Geneious type: kozak"
                     /label="kozak"
     CDS             3678..4394
                     /label="Venus YFP"
     CDS             4458..5369
                     /label="SMASh(AI) tag"
     misc_feature    4683..4685
                     /note="Geneious type: Mutation"
                     /label="V36M"
     misc_feature    4737..4739
                     /note="Geneious type: Mutation"
                     /label="T54A"
     misc_feature    4941..4943
                     /note="Geneious type: Mutation"
                     /label="S122G"
     misc_feature    5370..5396
                     /note="Geneious type: affinity tag"
                     /label="HA tag"
     misc_feature    5436..5438
                     /note="Geneious type: stop codon"
                     /label="STOP"
ORIGIN      
        1 ggtttgcgta ttggctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
       61 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
      121 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      181 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      241 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      301 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
      361 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
      421 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
      481 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
      541 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      601 cattcaaata tgtatccgct catgccaggt cttggactgg tgagaacggc ttgctcggca
      661 gcttcgatgt gtgctggagg gagaataaag gtctaagatg tgcgatagag ggaagtcgca
      721 ttgaattatg tgctgtgtag ggatcgctgg tatcaaatat gtgtgcccac ccctggcatg
      781 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      841 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      901 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      961 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1021 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1081 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1141 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1201 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1261 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1321 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1381 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1441 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1501 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1561 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1621 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1681 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1741 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgcc ataacttcgt
     1801 ataatgtatg ctatacgaag ttatggcatg accaaaatcc cttaacgtga gttttcgttc
     1861 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1921 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1981 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2041 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2101 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2161 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2221 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2281 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2341 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2401 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2461 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2521 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2581 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2641 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2701 gcgcgttggc cgattcatta atgcagagct tgcaattcgc gcgtttttca atattattga
     2761 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2821 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2881 attattatca tgacattaac ctataaaaat aggcgtatta cgaggccctt tcacgcatta
     2941 gatgcatgtc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3001 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     3061 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     3121 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     3181 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     3241 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     3301 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     3361 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3421 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3481 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
     3541 ccggactcta gcctaggccg cgggacggat aacaatttca cacaggaaac agctatgacc
     3601 attaggccta tttaggtgac actatagaac aagtttgtac aaaaaagcag gctggtaccg
     3661 gtccggaatt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3721 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     3781 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc
     3841 ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct
     3901 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     3961 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4021 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4081 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg
     4141 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
     4201 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4261 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
     4321 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4381 acgagctgta caagcccggg cccatggcca gcatgactgg tggacagcaa atggggtcga
     4441 cgctctacca ggagttcgat gagatggaag agtgctctca gcacttaccc ggcgccggca
     4501 gtagtggcga tatcatggat tacaaggatg acgacgataa gggctcttcc gggacaggct
     4561 ccggatccgg cactagtgcg cccatcacgg cgtacgccca gcagacgaga ggcctcctag
     4621 ggtgtataat caccagcctg actggccggg acaaaaacca agtggagggt gaggtccaga
     4681 tcatgtcaac tgctacccaa accttcctgg caacgtgcat caatggggta tgctgggcag
     4741 tctaccacgg ggccggaacg aggaccatcg catcacccaa gggtcctgtc atccagatgt
     4801 ataccaatgt ggaccaagac cttgtgggct ggcccgctcc tcaaggttcc cgctcattga
     4861 caccctgtac ctgcggctcc tcggaccttt acctggtcac gaggcacgcc gatgtcattc
     4921 ccgtgcgccg gcgaggtgat ggcaggggta gcctgctttc gccccggccc atttcctact
     4981 tgaaaggctc ctcggggggt ccgctgttgt gccccgcggg acacgccgtg ggcctattca
     5041 gggccgcggt gtgcacccgt ggagtggcta aagcggtgga ctttatccct gtggagaacc
     5101 tagagacaac catgagatcc ccggtgttca cggacaactc ctctccacca gcagtcaccc
     5161 tgacgcaccc aatcaccaaa atcgatacca aatacatcat gacatgcatg tcggccgacc
     5221 tggaggtcgt cacgagcacc tgggtgctcg ttggcggcgt cctggctgct ctggccgcgt
     5281 attgcctgtc aacaggctgc gtggtcatag tgggcaggat cgtcttgtcc gggaagccgg
     5341 caattatacc tgacagggag gttctctact atccctacga tgtgcccgat tacgctggcg
     5401 cgccttcagt agccagtgac aggacccagc ctgaatagag gggccagccc agggtcccca
     5461 gcccgcctgc cacacccagc ctgtggcttt tgccaactag gacttgagct ggggctgact
     5521 cccaaaggga tgccctgtcc agccagacat ctactcaccc actgacctgg cctgactcac
     5581 aactgccaca caaccatgct acatggactg aacatcaaga agcccctttc ccatagggct
     5641 cccacctgcc gcctacccct catctgtctg ccctggtcct ggcccccagc cccattggcc
     5701 tcaccctcta cactctcaga ccatcacaga acacgatctg gcttcctcat tctgctccag
     5761 tgtccagggc tctttgggta atcaagaaac caagtgtctg aaaggcaaca aaaagtagca
     5821 ccaaaaccca ggggacatcc tagggcagat gctaaagcag agatgctgag ggaacctcaa
     5881 cttccgggga tgcagcccac tcctgcagac acagcagatc cagctggtac cctagctgcc
     5941 gcccagggca accggccagt cttgggcagc atagctcccc tcccaggggt gagctgaagc
     6001 cacaaatgca gctgaagcag cagacctgac atcctggcac ctcctggccc ccaggtgagc
     6061 cctgcaccgt gtatcttaga gtgggggggg ggggcgtgca gaaccaagtc cactttccag
     6121 gagcccttca aggcttccct agcaaattag ttgcaagggt ggagtgaagt ggcatcagag
     6181 tgggtgtcat tgtagtgggc actggcactt cacggtccaa ggacggggct gaggtggggg
     6241 cggttctggg ggaatttgct aagccagctc accgaggacc ctgtgatttc tcaccccagc
     6301 agtgattcat accagtgaag aagagcagag ctcagctccg tgactcagcc atggcaggcg
     6361 gagggtcccc gaggggctga gtcctcacac ccagccgagg cagcggctgg agcctacaga
     6421 gccaggagaa tgacaccagg taagaaagtc ttgcctccta tcctgaccaa gcctcagatc
     6481 ccagccactc tccagatttc tgggttatcc gggaagtcct gacaggcccc aggggtacca
     6541 gctgggagca gggtaacctg gtgtccctcc tggatctagt ggtgagcagt tctctcacca
     6601 tcttgctgct tcctgtgtcc ttcaggtctc aaggctgctg agaagtcttt gctgccatgt
     6661 ctggaaaggg catcaccaca ccaccagcac cagcaccatc ctctcttctc ctgaagctgc
     6721 ctacatgggt tccaagacac tttcaaggca gagaaaacaa gattacagag aggaggtgcc
     6781 tggcaggggg cagcacccca gctcagccca agagctgaag gtgaagacaa gccagcatga
     6841 aaccacgggt ctgccatgat gcccgccccg ctggccgctc actagctgcc tgccattagc
     6901 ctctgcagct tgagcagggt ctgtaccctt ggtcctcttg gcacagagcc cagctcgctg
     6961 catggccttt ggctccccga ccagaccctt gcaggagcct taaggcttgg gcccctgccc
     7021 agcctgatct ttcctgctgt gccctgcctg ccaggtcaag cccagtccca ggagacccca
     7081 ggccttggct cctaggctgt tccatgaacc tccctgacct gcctggtgat tgcccagctg
     7141 aaacctcagc caagccccag ctccaattac cttgtgctgg tagctgcttg tgtctgcagt
     7201 ctgagtaggc cttgttgcgg ttcctccatc tgcctggtct attggtgttc tgagaccaat
     7261 tccactgatg ttctgacaga tcctccaccc tgtgcccctg ccagccccca caagtttatt
     7321 tttgcacaaa agccatgacc catactcatt tggctggcat agggtgtgta ggtaggccct
     7381 ggggactagg gagaccctgg agatctcaag agagtgtggc tatcccctat tttcaccaag
     7441 ccttgaatat ccagccaggc tgtctgccca taccatctta cctcaaagac agatatatat
     7501 ctatatatga ttttgttaat aaaactatga aacttattgg agtatgtgcc atttctcgag
     7561 ggtgtgggat gggctgaagc aggacctcag aggacactgc tcaaggaggt acaggaacac
     7621 ccaccgccta tgtaccccag aaaaacttct aagtctagag tatccctcga ggggcccaag
     7681 cttacgcgta cccagctttc ttgtacaaag tggtccctat agtgagtcgt attataagct
     7741 aggcactggc cgtcgtttta caacgtcgtg actgggaaaa ctgctagctt gggatctttg
     7801 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     7861 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactagct gcatatgctt
     7921 gctgcttgag agttttgctt actgagtatg atttatgaaa atattataca caggagctag
     7981 tgattctaat tgtttgtgta ttttagattc acagtcccaa ggctcatttc aggcccctca
     8041 gtcctcacag tctgttcatg atcataatca gccataccac atttgtagag gttttacttg
     8101 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     8161 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8221 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8281 tatcttatca tgtctggatc gatcctgcat taatgaatcg gccaacgcgc ggggagaggc
//
