LOCUS       pCS6-ZF(VEGFA)-S        7834 bp    DNA     circular UNA 24-MAY-2018
DEFINITION  pCS6 vector based.
ACCESSION   urn.local...91qqbh7-n7
VERSION     urn.local...91qqbh7-n7
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..527
                     /label="CMV promoter"
     misc_feature    723..728
                     /note="Geneious type: kozak"
                     /label="kozak"
     CDS             729..1010
                     /label="Zinc Finger (VEGFA)"
     misc_feature    1038..1064
                     /note="Geneious type: Affinity Tag"
                     /label="HA Tag"
     misc_feature    1083..1103
                     /note="Geneious type: Cleavage site"
                     /label="Cleavage site"
     CDS             1083..1790
                     /label="StaPL(AI) module"
     misc_feature    1287..1289
                     /note="Geneious type: Mutation"
                     /label="V36M"
     misc_feature    1341..1343
                     /note="Geneious type: Mutation"
                     /label="T54A"
     misc_feature    1545..1547
                     /note="Geneious type: Mutation"
                     /label="S122G"
     CDS             1791..2507
                     /label="Venus YFP"
     CDS             2529..4085
                     /label="VPR"
     misc_feature    2685..2702
                     /note="Geneious type: Mutation"
                     /label="Internal NLS removed"
     misc_feature    4104..4124
                     /note="Geneious type: Cleavage site"
                     /label="Cleavage site"
     CDS             4149..4196
                     /label="NLS"
     misc_feature    4197..4199
                     /note="Geneious type: stop_codon"
                     /note="Geneious type: Stop codon"
                     /label="STOP"
     rep_origin      4971..5426
                     /label="M13 Origin"
     CDS             5714..6574
                     /label="AmpR"
ORIGIN      
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agcctaggcc gcggagcgga taacaatttc acacaggaaa cagctatgac cattaggcct
      661 atttaggtga cactatagaa caagtttgta caaaaaagca ggctggtacc ggtccggaat
      721 tcgccaccat gggcaaaaag aagcagcaca tctgccacat ccagggctgc ggcaaggtgt
      781 acggcaccac cagcaacctg aggaggcacc ttaggtggca caccggggag aggcccttca
      841 tgtgcacctg gagctactgc ggaaaaaggt tcaccaggag cagcaacttg cagaggcaca
      901 agaggaccca cacaggcgag aagaagttcg cctgccccga gtgccccaag aggtttatga
      961 ggagcgacca tctgagcagg cacatcaaga cccaccagaa caagaaaggc gggggcagtg
     1021 gcgggagcgg gcgcgcctat ccctacgatg tgcccgatta cgctggtgga tccggcgggt
     1081 cagatgagat ggaagagtgc tctcagcacg gcggatctgg aggttctaca ggctgcgtgg
     1141 tcatagtggg caggatcgtc ttgtccggat ccggcactag tgcgcccatc acggcgtacg
     1201 cccagcagac gagaggcctc ctagggtgta taatcaccag cctgactggc cgggacaaaa
     1261 accaagtgga gggtgaggtc cagatcatgt caactgctac ccaaaccttc ctggcaacgt
     1321 gcatcaatgg ggtatgctgg gcagtctacc acggggccgg aacgaggacc atcgcatcac
     1381 ccaagggtcc tgtcatccag atgtatacca atgtggacca agaccttgtg ggctggcccg
     1441 ctcctcaagg ttcccgctca ttgacaccct gtacctgcgg ctcctcggac ctttacctgg
     1501 tcacgaggca cgccgatgtc attcccgtgc gccggcgagg tgatggcagg ggtagcctgc
     1561 tttcgccccg gcccatttcc tacttgaaag gctcctcggg gggtccgctg ttgtgccccg
     1621 cgggacacgc cgtgggccta ttcagggccg cggtgtgcac ccgtggagtg gctaaagcgg
     1681 tggactttat ccctgtggag aacctagaga caaccatgag atccccggtg ttcacggaca
     1741 actcctctcc accagcagtc accctgacgc acgggggttc aggcggtagt atggtgagca
     1801 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1861 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1921 ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1981 ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     2041 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2101 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2161 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2221 acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     2281 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
     2341 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     2401 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     2461 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggga ggctccggag
     2521 gtagcggcga tgctttagac gattttgact tagatatgct tggttcagac gcgttagacg
     2581 acttcgacct agacatgtta ggctcagatg cattggacga cttcgattta gatatgttgg
     2641 gctccgatgc cctagatgac tttgatctag atatgctagg tagtggagga tccggaggat
     2701 cccagtatct gcccgacaca gatgatagac accgaatcga agagaaacgc aagcgaacgt
     2761 atgaaacctt caaatcgatc atgaagaaat cgcccttctc gggtccgacc gatcccaggc
     2821 ccccaccgag aaggattgcg gtcccgtccc gctcgtcggc cagcgtgccg aagcctgcgc
     2881 cgcagcccta ccccttcacg tcgagcctga gcacaatcaa ttatgacgag ttcccgacga
     2941 tggtgttccc ctcgggacaa atctcacaag cctcggcgct cgcaccagcg cctccccaag
     3001 tccttccgca agcgcctgcc ccagcgcctg caccggcaat ggtgtccgcc ctcgcacagg
     3061 cccctgcgcc cgtccccgtg ctcgcgcctg gaccgcccca ggcggtcgct ccaccggctc
     3121 cgaagccgac gcaggccgga gagggaacac tctccgaagc acttcttcaa ctccagtttg
     3181 atgacgagga tcttggagca ctccttggaa actcgacaga ccctgcggtg tttaccgacc
     3241 tcgcgtcagt agataactcc gaatttcagc agcttttgaa ccagggtatc ccggtcgcgc
     3301 cacatacaac ggagcccatg ttgatggaat accccgaagc aatcacgaga cttgtgacgg
     3361 gagcgcagcg gcctcccgat cccgcacccg cacctttggg ggcacctggc ctccctaacg
     3421 gacttttgag cggcgacgag gatttctcct ccatcgccga tatggatttc tcagccttgc
     3481 tgtcacagat ttccagcggc tctggcagcg gcagccggga ttccagggaa gggatgtttt
     3541 tgccgaagcc tgaggccggc tccgctatta gtgacgtgtt tgagggccgc gaggtgtgcc
     3601 agccaaaacg aatccggcca tttcatcctc caggaagtcc atgggccaac cgcccactcc
     3661 ccgccagcct cgcaccaaca ccaaccggtc cagtacatga gccagtcggg tcactgaccc
     3721 cggcaccagt ccctcagcca ctggacccag cgcccgcagt gactcccgag gccagtcacc
     3781 tgttggagga ccccgatgaa gagacgagcc aggctgtcaa agcccttcgg gagatggccg
     3841 atactgtgat tccccagaag gaagaggctg caatctgtgg ccaaatggac ctttcccatc
     3901 cgcccccaag gggccatctg gatgagctga caaccacact tgagtccatg accgaggatc
     3961 tgaacctgga ctcacccctg accccggaat tgaacgagat tctggatacc ttcctgaacg
     4021 acgagtgcct cttgcatgcc atgcatatca gcacaggact gtccatcttc gacacatctc
     4081 tgtttggcgg atcaggcggc tccgatgaga tggaagagtg ctctcagcac ggcggctccg
     4141 gcggctccga ccctaagaaa aaacggaaag ttgatccaaa aaagaagaga aaggtatagc
     4201 tagagtatcc ctcgaggggc ccaagcttac gcgtacccag ctttcttgta caaagtggtc
     4261 cctatagtga gtcgtattat aagctaggca ctggccgtcg ttttacaacg tcgtgactgg
     4321 gaaaactgct agcttgggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     4381 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     4441 tgtgttaaac tagctgcata tgcttgctgc ttgagagttt tgcttactga gtatgattta
     4501 tgaaaatatt atacacagga gctagtgatt ctaattgttt gtgtatttta gattcacagt
     4561 cccaaggctc atttcaggcc cctcagtcct cacagtctgt tcatgatcat aatcagccat
     4621 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     4681 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     4741 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4801 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcgatcc tgcattaatg
     4861 aatcggccaa cgcgcgggga gaggcggttt gcgtattggc tggcgtaata gcgaagaggc
     4921 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     4981 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5041 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5101 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5161 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5221 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5281 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5341 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5401 taacaaaata ttaacgttta caatttcagg tggcactttt cggggaaatg tgcgcggaac
     5461 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatgc caggtcttgg
     5521 actggtgaga acggcttgct cggcagcttc gatgtgtgct ggagggagaa taaaggtcta
     5581 agatgtgcga tagagggaag tcgcattgaa ttatgtgctg tgtagggatc gctggtatca
     5641 aatatgtgtg cccacccctg gcatgagaca ataaccctga taaatgcttc aataatattg
     5701 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5761 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5821 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5881 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5941 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6001 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6061 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6121 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6181 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6241 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6301 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6361 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6421 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6481 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6541 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6601 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6661 tgataatctc atgccataac ttcgtataat gtatgctata cgaagttatg gcatgaccaa
     6721 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6781 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6841 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6901 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6961 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7021 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7081 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7141 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     7201 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7261 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7321 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7381 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7441 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7501 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7561 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gagcttgcaa
     7621 ttcgcgcgtt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     7801 tagtacgagg ccctttcact cattagatgc atgt
//
