LOCUS       pCS6-ZF(VEGFA)-S        7276 bp    DNA     circular UNA 24-MAY-2018
DEFINITION  pCS6 vector based.
ACCESSION   urn.local...91qczkh-9u
VERSION     urn.local...91qczkh-9u
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..527
                     /label="CMV promoter"
     misc_feature    723..728
                     /note="Geneious type: kozak"
                     /label="kozak"
     CDS             729..1010
                     /label="Zinc Finger (VEGFA)"
     misc_feature    1038..1064
                     /note="Geneious type: Affinity Tag"
                     /label="HA Tag"
     misc_feature    1083..1103
                     /note="Geneious type: Cleavage site"
                     /label="Cleavage site"
     CDS             1083..1790
                     /label="StaPL(TI) module"
     misc_feature    1308..1310
                     /note="Geneious type: Mutation"
                     /label="F43L"
     misc_feature    1419..1421
                     /note="Geneious type: Mutation"
                     /label="Q80K"
     misc_feature    1545..1547
                     /note="Geneious type: Mutation"
                     /label="S122R"
     misc_feature    1683..1685
                     /note="Geneious type: Mutation"
                     /label="D168Y"
     CDS             1791..3218
                     /label="tdTomato RFP"
     CDS             3237..3527
                     /label="KRAB"
     misc_feature    3546..3566
                     /note="Geneious type: Cleavage site"
                     /label="Cleavage site"
     CDS             3591..3638
                     /label="NLS"
     misc_feature    3639..3641
                     /note="Geneious type: stop_codon"
                     /note="Geneious type: Stop codon"
                     /label="STOP"
     rep_origin      4413..4868
                     /label="M13 Origin"
     CDS             5156..6016
                     /label="AmpR"
ORIGIN      
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agcctaggcc gcggagcgga taacaatttc acacaggaaa cagctatgac cattaggcct
      661 atttaggtga cactatagaa caagtttgta caaaaaagca ggctggtacc ggtccggaat
      721 tcgccaccat gggcaaaaag aagcagcaca tctgccacat ccagggatgc ggcaaggtgt
      781 acggcaccac cagcaacctg aggaggcacc ttaggtggca caccggggag aggcccttca
      841 tgtgcacctg gagctactgc ggaaaaaggt tcaccaggag cagcaacttg cagaggcaca
      901 agaggaccca cacaggcgag aagaagttcg cctgccccga gtgccccaag aggtttatga
      961 ggagcgacca tctgagcagg cacatcaaga cccaccagaa caagaaaggc gggggcagtg
     1021 gcgggagcgg gcgcgcctat ccctacgatg tgcccgatta cgctggtgga tccggcgggt
     1081 cagatgagat ggaagagtgc tctcagcacg gcggatctgg aggttctaca ggctgcgtgg
     1141 tcatagtggg caggatcgtc ttgtccggat ccggcactag tgcgcccatc acggcgtacg
     1201 cccagcagac gagaggcctc ctagggtgta taatcaccag cctgactggc cgggacaaaa
     1261 accaagtgga gggtgaggtc cagatcgtgt caactgctac ccaaaccttg ctggcaacgt
     1321 gcatcaatgg ggtatgctgg acagtctacc acggggccgg aacgaggacc atcgcatcac
     1381 ccaagggtcc tgtcatccag atgtatacca atgtggacaa agaccttgtg ggctggcccg
     1441 ctcctcaagg ttcccgctca ttgacaccct gtacctgcgg ctcctcggac ctttacctgg
     1501 tcacgaggca cgccgatgtc attcccgtgc gccggcgagg tgatcgcagg ggtagcctgc
     1561 tttcgccccg gcccatttcc tacttgaaag gctcctcggg gggtccgctg ttgtgccccg
     1621 cgggacacgc cgtgggccta ttcagggccg cggtgtgcac ccgtggagtg gctaaagcgg
     1681 tgtactttat ccctgtggag aacctagaga caaccatgag atccccggtg ttcacggaca
     1741 actcctctcc accagcagtc accctgacgc acgggggttc aggcggtagt atggtgagca
     1801 agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga
     1861 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1921 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
     1981 cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca
     2041 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     2101 gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga
     2161 agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct
     2221 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc
     2281 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg
     2341 ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct
     2401 cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc
     2461 tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg
     2521 aggacaacaa catggccgtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct
     2581 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     2641 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     2701 tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg
     2761 attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     2821 acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca
     2881 aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca
     2941 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga
     3001 tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct
     3061 acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca
     3121 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc
     3181 accacctgtt cctgtacggc atggacgagc tgtacaaggg aggctccgga ggtagcatgg
     3241 acgccaagag cctgaccgcc tggagcagga cactggtgac cttcaaggac gtgttcgtgg
     3301 acttcacccg agaggagtgg aagctgctgg acaccgccca gcagatcgtg tacaggaacg
     3361 tgatgctgga aaactacaag aacctggtga gcctgggcta ccagctgacc aagcccgacg
     3421 tgatcctgag gctggagaag ggcgaggagc cctggttggt ggaaagggag atccaccagg
     3481 agacccaccc cgacagcgag accgcatttg agatcaagag cagcgtgggc ggatcaggcg
     3541 gctccgatga gatggaagag tgctctcagc acggcggctc cggcggctcc gaccctaaga
     3601 aaaaacggaa agttgatcca aaaaagaaga gaaaggtata gctagagtat ccctcgaggg
     3661 gcccaagctt acgcgtaccc agctttcttg tacaaagtgg tccctatagt gagtcgtatt
     3721 ataagctagg cactggccgt cgttttacaa cgtcgtgact gggaaaactg ctagcttggg
     3781 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     3841 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actagctgca
     3901 tatgcttgct gcttgagagt tttgcttact gagtatgatt tatgaaaata ttatacacag
     3961 gagctagtga ttctaattgt ttgtgtattt tagattcaca gtcccaaggc tcatttcagg
     4021 cccctcagtc ctcacagtct gttcatgatc ataatcagcc ataccacatt tgtagaggtt
     4081 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     4141 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     4201 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     4261 atcaatgtat cttatcatgt ctggatcgat cctgcattaa tgaatcggcc aacgcgcggg
     4321 gagaggcggt ttgcgtattg gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4381 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     4441 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4501 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4561 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4621 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4681 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4741 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4801 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4861 tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4921 ctaaatacat tcaaatatgt atccgctcat gccaggtctt ggactggtga gaacggcttg
     4981 ctcggcagct tcgatgtgtg ctggagggag aataaaggtc taagatgtgc gatagaggga
     5041 agtcgcattg aattatgtgc tgtgtaggga tcgctggtat caaatatgtg tgcccacccc
     5101 tggcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5161 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5221 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5281 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5341 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5401 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5461 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5521 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5581 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5641 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5701 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5761 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5821 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5881 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5941 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6001 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6061 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgccata
     6121 acttcgtata atgtatgcta tacgaagtta tggcatgacc aaaatccctt aacgtgagtt
     6181 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6241 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6301 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6361 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6421 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6481 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6541 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6601 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6661 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6721 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6781 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6841 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6901 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6961 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7021 tctccccgcg cgttggccga ttcattaatg cagagcttgc aattcgcgcg tttttcaata
     7081 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7141 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     7201 agaaaccatt attatcatga cattaaccta taaaaatagg cgtagtacga ggccctttca
     7261 ctcattagat gcatgt
//
