LOCUS       VPR-dSpCas9[StaP       13958 bp ds-DNA     circular     29-MAY-2018
DEFINITION  .
ACCESSION   urn.local...92rbig6-r
VERSION
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     promoter        8..1243
                     /gene="CAGp"
                     /note="PROMOTER CAG promoter"
                     /note="CAG promoter"
                     /label="CAG promoter"
     CDS             1359..1955
                     /label="PuroR"
     enhancer        1995..2586
                     /label="WPRE"
     promoter        2605..3104
                     /label="PGK promoter"
     misc_feature    3128..3133
                     /note="Geneious type: Kozak"
                     /label="kozak"
     CDS             3134..4702
                     /label="VPR"
     misc_feature    3293..3319
                     /note="Geneious type: Mutation"
                     /label="Internal NLS removed"
     CDS             4730..5428
                     /label="TagBFP"
     CDS             5450..9187
                     /label="dSpCas9 N-term"
     misc_feature    5477..5479
                     /note="Geneious type: Mutation"
                     /label="D10A"
     misc_feature    7967..7969
                     /note="Geneious type: Mutation"
                     /label="H840A"
     misc_feature    9215..9241
                     /note="Geneious type: Affinity Tag"
                     /label="HA Tag"
     misc_feature    9260..9280
                     /note="Geneious type: Cleavage site"
                     /label="Cleavage site"
     CDS             9260..9967
                     /label="StaPL(TI) module"
     misc_feature    9485..9487
                     /note="Geneious type: Mutation"
                     /label="F43L"
     misc_feature    9596..9598
                     /note="Geneious type: Mutation"
                     /label="Q80K"
     misc_feature    9722..9724
                     /note="Geneious type: Mutation"
                     /label="S122R"
     misc_feature    9860..9862
                     /note="Geneious type: Mutation"
                     /label="D168Y"
     CDS             9968..10333
                     /label="dSpCas9 C-term"
     misc_feature    10337..10363
                     /note="Geneious type: Affinity Tag"
                     /label="HA tag"
     CDS             10382..10432
                     /label="NLS"
     misc_feature    10457..10459
                     /note="Geneious type: Stop codon"
                     /label="STOP"
     promoter        10471..10718
                     /gene="3' PB"
                     /note="PROMOTER 3' PB repeat"
                     /note="3' PB repeat"
                     /note="Geneious type: Piggyback"
                     /label="3' PB"
     misc_feature    complement(11200..11788)
                     /note="Geneious type: Origin"
                     /label="ori"
     CDS             complement(11962..12819)
                     /gene="AmpR"
                     /note="CDS beta-lactamase AmpR confers resistance to
                     ampici llin, carbenicillin, and related antibiotics"
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGAS LIKHW"
                     /label="AmpR"
     misc_feature    complement(12950..13405)
                     /note="Geneious type: Origin"
                     /label="f1 ori"
     promoter        13617..13938
                     /gene="5' PB"
                     /note="PROMOTER 5' PB repeat"
                     /note="5' PB repeat"
                     /note="Geneious type: Piggyback"
                     /label="5' PB"
ORIGIN      
        1 ccatgggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
       61 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      121 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
      181 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
      241 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc
      301 ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc
      361 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
      421 ggtttaatga cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga
      481 gggccctttg tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
      541 gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
      601 ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
      661 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
      721 gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag
      781 cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg
      841 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
      901 ggctcggggg aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag
     1081 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     1141 cgtccccttc tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg
     1201 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     1261 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttgttgtgc
     1321 tgtctcatca ttttggcaaa gaattcgcca ccatgaccat gaccgagtac aagcccacgg
     1381 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     1441 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     1501 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     1561 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     1621 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     1681 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     1741 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     1801 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     1861 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     1921 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt ttaaaccatg caagcttgat
     1981 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2041 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2101 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2161 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2221 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2281 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2341 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2401 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2461 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2521 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2581 ccgcatcctg caggtaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     2641 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     2701 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     2761 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     2821 ggacgtgaca aatggaagta gcacgtctca ctaggctcgt gcagatggac agcaccgctg
     2881 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     2941 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     3001 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     3061 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     3121 tgtcgacgcc accatggatg ctttagacga ttttgactta gatatgcttg gttcagacgc
     3181 gttagacgac ttcgacctag acatgttagg ctcagatgca ttggacgact tcgatttaga
     3241 tatgttgggc tccgatgccc tagatgactt tgatctagat atgctaggta gtggagggtc
     3301 aggtggtagt ggcggatccc agtatctgcc cgacacagat gatagacacc gaatcgaaga
     3361 gaaacgcaag cgaacgtatg aaaccttcaa atcgatcatg aagaaatcgc ccttctcggg
     3421 tccgaccgat cccaggcccc caccgagaag gattgcggtc ccgtcccgct cgtcggccag
     3481 cgtgccgaag cctgcgccgc agccctaccc cttcacgtcg agcctgagca caatcaatta
     3541 tgacgagttc ccgacgatgg tgttcccctc gggacaaatc tcacaagcct cggcgctcgc
     3601 accagcgcct ccccaagtcc ttccgcaagc gcctgcccca gcgcctgcac cggcaatggt
     3661 gtccgccctc gcacaggccc ctgcgcccgt ccccgtgctc gcgcctggac cgccccaggc
     3721 ggtcgctcca ccggctccga agccgacgca ggccggagag ggaacactct ccgaagcact
     3781 tcttcaactc cagtttgatg acgaggatct tggagcactc cttggaaact cgacagaccc
     3841 tgcggtgttt accgacctcg cgtcagtaga taactccgaa tttcagcagc ttttgaacca
     3901 gggtatcccg gtcgcgccac atacaacgga gcccatgttg atggaatacc ccgaagcaat
     3961 cacgagactt gtgacgggag cgcagcggcc tcccgatccc gcacccgcac ctttgggggc
     4021 acctggcctc cctaacggac ttttgagcgg cgacgaggat ttctcctcca tcgccgatat
     4081 ggatttctca gccttgctgt cacagatttc cagcggctct ggcagcggca gccgggattc
     4141 cagggaaggg atgtttttgc cgaagcctga ggccggctcc gctattagtg acgtgtttga
     4201 gggccgcgag gtgtgccagc caaaacgaat ccggccattt catcctccag gaagtccatg
     4261 ggccaaccgc ccactccccg ccagcctcgc accaacacca accggtccag tacatgagcc
     4321 agtcgggtca ctgaccccgg caccagtccc tcagccactg gatccagcgc ccgcagtgac
     4381 tcccgaggcc agtcacctgt tggaggatcc cgatgaagag acgagccagg ctgtcaaagc
     4441 ccttcgggag atggccgata ctgtgattcc ccagaaggaa gaggctgcaa tctgtggcca
     4501 aatggacctt tcccatccgc ccccaagggg ccatctggat gagctgacaa ccacacttga
     4561 gtccatgacc gaggatctga acctggactc acccctgacc ccggaattga acgagattct
     4621 ggataccttc ctgaacgacg agtgcctctt gcatgccatg catatcagca caggactgtc
     4681 catcttcgac acatctctgt ttcatatgtc aagcgctgca ggtgctacca tgagcgagct
     4741 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
     4801 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     4861 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
     4921 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
     4981 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     5041 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     5101 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
     5161 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
     5221 cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc
     5281 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga
     5341 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
     5401 ccctagcaaa ctggggcaca agcttaattc aagcgctgca ggtgccacca tggacaagaa
     5461 gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga
     5521 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa
     5581 gaagaacctg atcggcgccc tgctgttcga cagcggagaa acagccgagg ccacccggct
     5641 gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga
     5701 gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc
     5761 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga
     5821 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga
     5881 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt
     5941 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct
     6001 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag
     6061 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa
     6121 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggcaacc tgattgccct
     6181 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact
     6241 gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga
     6301 ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga
     6361 catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag
     6421 atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc
     6481 tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacatcga
     6541 tggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga
     6601 cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac
     6661 cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg
     6721 gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct
     6781 gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg
     6841 gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa
     6901 gggcgccagc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa
     6961 cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt acaacgagct
     7021 gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca
     7081 gaaaaaagcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct
     7141 gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga
     7201 agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa
     7261 ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac
     7321 actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga
     7381 cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg
     7441 gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa
     7501 gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt
     7561 taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat
     7621 tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt
     7681 ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc
     7741 cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat
     7801 cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac
     7861 ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt
     7921 ggaccaggaa ctggacatca accggctgtc cgactacgat gtggacgcta tcgtgcctca
     7981 gagctttctg aaggacgact ccatcgataa caaagtgctg actcggagcg acaagaaccg
     8041 gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg
     8101 ccagctgctg aatgccaagc tgattaccca gaggaagttc gacaatctga ccaaggccga
     8161 gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac
     8221 ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga
     8281 cgagaacgac aaactgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc
     8341 cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc
     8401 ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct
     8461 ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa
     8521 gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa
     8581 ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga
     8641 gacaaacggc gaaacaggcg agatcgtgtg ggataagggc cgggactttg ccaccgtgcg
     8701 gaaagtgctg tctatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg
     8761 cttcagcaaa gagtctatcc tgcccaagag gaacagcgac aagctgatcg ccagaaagaa
     8821 ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct
     8881 ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct
     8941 ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc
     9001 caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt
     9061 cgagctggaa aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa
     9121 cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa
     9181 gctgaagggg ggcagtggcg ggagcgggcg cgcctatccc tacgatgtgc ccgattacgc
     9241 tggtggatcc ggcgggtcag atgagatgga agagtgctct cagcacggcg gatctggagg
     9301 ttctacaggc tgcgtggtca tagtgggcag gatcgtcttg tccggatccg gcactagtgc
     9361 gcccatcacg gcgtacgccc agcagacgag aggcctccta gggtgtataa tcaccagcct
     9421 gactggccgg gacaaaaacc aagtggaggg tgaggtccag atcgtgtcaa ctgctaccca
     9481 aaccttgctg gcaacgtgca tcaatggggt atgctggaca gtctaccacg gggccggaac
     9541 gaggaccatc gcatcaccca agggtcctgt catccagatg tataccaatg tggacaaaga
     9601 ccttgtgggc tggcccgctc ctcaaggttc ccgctcattg acaccctgta cctgcggctc
     9661 ctcggacctt tacctggtca cgaggcacgc cgatgtcatt cccgtgcgcc ggcgaggtga
     9721 tcgcaggggt agcctgcttt cgccccggcc catttcctac ttgaaaggct cctcgggggg
     9781 tccgctgttg tgccccgcgg gacacgccgt gggcctattc agggccgcgg tgtgcacccg
     9841 tggagtggct aaagcggtgt actttatccc tgtggagaac ctagagacaa ccatgagatc
     9901 cccggtgttc acggacaact cctctccacc agcagtcacc ctgacgcacg ggggttcagg
     9961 cggtagtggc tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaaaca
    10021 ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga
    10081 cgctaatctg gacaaggtgc tgagcgccta caacaagcac agagacaagc ctatcagaga
    10141 gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt
    10201 caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga
    10261 cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca
    10321 gctgggaggc gacgcctatc cctatgacgt gcccgattat gccagcctgg gcagcggctc
    10381 ccccaagaaa aaacgcaagg tggaagatcc taagaaaaag cggaaagtgg acggcattgg
    10441 tagtgggagc aacggatgac tcgagctagt taaaagtttt gttactttat agaagaaatt
    10501 ttgagttttt gttttttttt aataaataaa taaacataaa taaattgttt gttgaattta
    10561 ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt aataaataaa
    10621 cctcgatata cagaccgata aaacacatgc gtcaatttta cgcatgatta tctttaacgt
    10681 acgtcacaat atgattatct ttctagggtt aatctagtat acgcgtatgc ggccgcttaa
    10741 ttaatccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
    10801 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    10861 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    10921 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    10981 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    11041 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    11101 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    11161 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    11221 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    11281 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    11341 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    11401 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    11461 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    11521 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    11581 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    11641 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    11701 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    11761 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    11821 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    11881 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    11941 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    12001 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    12061 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    12121 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    12181 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    12241 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    12301 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    12361 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    12421 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    12481 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    12541 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    12601 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    12661 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    12721 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    12781 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    12841 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    12901 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
    12961 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
    13021 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
    13081 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
    13141 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
    13201 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
    13261 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
    13321 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
    13381 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
    13441 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
    13501 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
    13561 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg ggcgcgccat tctagattaa
    13621 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
    13681 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
    13741 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt
    13801 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
    13861 ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt
    13921 atagatatca actagaatgc tagcatgggc ccagatct
//
