LOCUS Exported 10203 bp ds-DNA circular SYN 31-MAR-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 10203) AUTHORS Jared Toettcher TITLE Direct Submission JOURNAL Exported May 1, 2018 from SnapGene 4.1.8 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10203 /organism="recombinant plasmid" /mol_type="other DNA" misc_feature 550..729 /gene="cPPT" /label=cPPT misc_feature 705..728 /label=cPPT_5'seq promoter 762..1255 /gene="pSFFV" /label=SFFV /note="PROMOTER" misc_feature 1180..1203 /gene="*" /label=1166_seq primer_bind 1305..1340 /label=Fragment.FOR misc_recomb 1321..1324 /label=AttL1 CDS 1330..1581 /codon_start=1 /label=ErkKTR /translation="MKGRKPRDLELPLSPSLLGGQGPERTPGSGTSSGLQAPGPALSPS KRSGLEDPATPSKKPRTPSVSSRLERLTLQSSFQFPSTS" misc_feature join(1330..1332,1456..1461) /label=c-Jun(1-281) misc_feature 1333..1452 /label=ERKDS primer_bind complement(1559..1599) /label=Fragment.REV primer_bind 1584..1605 /label=EDPR17.FOR primer_bind 1596..1625 /label=Mutagenic Primer CDS 1600..2547 /codon_start=1 /gene="irFP" /label=iRFP /note="CDS" /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE" primer_bind complement(2525..2564) /label=EDPR18.REV misc_feature 2635..2657 /gene="**" /label=1167_seq misc_feature 2644..3235 /gene="wPRE" /label=WPRE gene 4466..5326 /gene="AmpR" /label=bla (AmpR) /note="CDS" primer_bind complement(7518..7531) /label=Fragment.FOR primer_bind 9371..9397 /label=Fragment.REV ORIGIN 1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc 61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg 121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg 181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc 241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt 301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct 361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat 421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag 481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat 541 ctcgacggtc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg 601 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa 661 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat 721 ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa taaaagattt 781 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta 841 gctgcagtaa cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt 901 cagatcaagg gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg 961 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc 1021 ccgaggccaa gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc 1081 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta 1141 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag 1201 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga 1261 tctggagctc tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcc 1321 ggctccacca tgaagggccg aaagcctcgg gacctggaac ttccacttag cccaagcctg 1381 ctgggtggcc agggacctga acggactcca ggatcaggaa caagctctgg tcttcaggca 1441 ccggggccag cgctttcgcc cagcaagcgt agcggtctcg aggacccagc gacgccatcg 1501 aagaagccac gtaccccatc ggtgtcgtca aggctggagc gcctgaccct ccagtccagc 1561 tttcaattcc catccactag tccggatccg gtcgccacca tggctgaagg ctccgtcgcc 1621 aggcagcctg acctcttgac ctgcgacgat gagccgatcc atatccccgg tgccatccaa 1681 ccgcatggac tgctgctcgc cctcgccgcc gacatgacga tcgttgccgg cagcgacaac 1741 cttcccgaac tcaccggact ggcgatcggc gccctgatcg gccgctctgc ggccgatgtc 1801 ttcgactcgg agacgcacaa ccgtctgacg atcgccttgg ccgagcccgg ggcggccgtc 1861 ggagcaccga tcactgtcgg cttcacgatg cgaaaggacg caggcttcat cggctcctgg 1921 catcgccatg atcagctcat cttcctcgag ctcgagcctc cccagcggga cgtcgccgag 1981 ccgcaggcgt tcttccgccg caccaacagc gccatccgcc gcctgcaggc cgccgaaacc 2041 ttggaaagcg cctgcgccgc cgcggcgcaa gaggtgcgga agattaccgg cttcgatcgg 2101 gtgatgatct atcgcttcgc ctccgacttc agcggcgaag tgatcgcaga ggatcggtgc 2161 gccgaggtcg agtcaaaact aggcctgcac tatcctgcct caaccgtgcc ggcgcaggcc 2221 cgtcggctct ataccatcaa cccggtacgg atcattcccg atatcaatta tcggccggtg 2281 ccggtcaccc cagacctcaa tccggtcacc gggcggccga ttgatcttag cttcgccatc 2341 ctgcgcagcg tctcgcccgt ccatctggaa ttcatgcgca acataggcat gcacggcacg 2401 atgtcgatct cgattttgcg cggcgagcga ctgtggggat tgatcgtttg ccatcaccga 2461 acgccgtact acgtcgatct cgatggccgc caagcctgcg agctagtcgc ccaggttctg 2521 gcctggcaga tcggcgtgat ggaagaggcg gccgcaactc ccacctgcaa catgcgtgac 2581 tgactgaggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat 2641 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 2701 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 2761 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 2821 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 2881 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 2941 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 3001 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct 3061 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 3121 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 3181 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata 3241 ccgtcgacca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 3301 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 3361 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 3421 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 3481 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat 3541 taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt 3601 attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct 3661 tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac 3721 gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca 3781 gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg 3841 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc 3901 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg 3961 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg 4021 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa 4081 tggtgcactc tcagtacaat ctagctctga tgccgcatag ttaagccagc cccgacaccc 4141 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca 4201 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg 4261 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat 4321 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 4381 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 4441 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 4501 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 4561 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 4621 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 4681 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 4741 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 4801 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 4861 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 4921 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 4981 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 5041 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 5101 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 5161 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 5221 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 5281 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 5341 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 5401 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 5461 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 5521 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 5581 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 5641 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 5701 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 5761 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 5821 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 5881 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 5941 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 6001 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 6061 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 6121 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 6181 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 6241 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 6301 ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 6361 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 6421 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 6481 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 6541 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 6601 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 6661 aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc 6721 agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc 6781 agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa 6841 acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat 6901 ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc 6961 cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat 7021 gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg 7081 aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt 7141 ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca 7201 aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg 7261 attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct 7321 tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt 7381 tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt 7441 caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc 7501 acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat 7561 taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc 7621 acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg 7681 ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact 7741 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata 7801 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga 7861 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc 7921 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt 7981 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc 8041 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca 8101 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct 8161 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc 8221 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt 8281 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc 8341 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 8401 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 8461 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 8521 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact 8581 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac 8641 ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa 8701 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa 8761 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc 8821 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc 8881 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt 8941 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt 9001 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga 9061 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg 9121 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt 9181 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 9241 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 9301 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 9361 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct 9421 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg 9481 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg 9541 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg 9601 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg 9661 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac 9721 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc 9781 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag 9841 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggtgat cttcagacct 9901 ggacgatata tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa 9961 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa 10021 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg 10081 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc 10141 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct 10201 ggg //