LOCUS       pCS6-StaPLd-Casp        6801 bp    DNA     circular UNA 25-MAY-2018
DEFINITION  null (Circularized).
ACCESSION   urn.local...925ncak-3h
VERSION     urn.local...925ncak-3h
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     CDS             164..1024
                     /label="AmpR"
     misc_feature    2284^2285
                     /note="Geneious type: origin"
                     /label="origin"
     promoter        2285..2811
                     /label="CMV promoter"
     misc_feature    3007..3012
                     /note="Geneious type: kozak"
                     /label="kozak"
     CDS             3013..3852
                     /label="Casp9 large/small #1"
     misc_feature    3880..3906
                     /note="Geneious type: Affinity Tag"
                     /label="HA Tag"
     misc_feature    3925..3945
                     /note="Geneious type: Cleavage site"
                     /label="Cleavage site"
     CDS             3925..4632
                     /label="StaPL(AI) module"
     misc_feature    4129..4131
                     /note="Geneious type: Mutation"
                     /label="V36M"
     misc_feature    4183..4185
                     /note="Geneious type: Mutation"
                     /label="T54A"
     misc_feature    4387..4389
                     /note="Geneious type: Mutation"
                     /label="S122G"
     CDS             4633..5469
                     /label="Casp9 large/small #2"
     misc_feature    5470..5472
                     /note="Geneious type: Stop codon"
                     /label="STOP"
     rep_origin      6222..6677
                     /label="M13 Origin"
ORIGIN      
        1 gatgtgtgct ggagggagaa taaaggtcta agatgtgcga tagagggaag tcgcattgaa
       61 ttatgtgctg tgtagggatc gctggtatca aatatgtgtg cccacccctg gcatgagaca
      121 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      181 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      241 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      301 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      361 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      421 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      481 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      541 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
      601 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
      661 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
      721 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
      781 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
      841 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
      901 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
      961 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1021 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1081 atttaaaagg atctaggtga agatcctttt tgataatctc atgccataac ttcgtataat
     1141 gtatgctata cgaagttatg gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1201 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1261 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1321 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1381 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1441 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1501 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1561 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1621 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1681 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1741 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1801 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1861 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1921 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     1981 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2041 ttggccgatt cattaatgca gagcttgcaa ttcgcgcgtt tttcaatatt attgaagcat
     2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     2221 tatcatgaca ttaacctata aaaataggcg tagtacgagg ccctttcact cattagatgc
     2281 atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     2341 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     2401 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     2461 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     2521 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     2581 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     2641 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     2701 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     2761 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     2821 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
     2881 ctctagccta ggccgcggag cggataacaa tttcacacag gaaacagcta tgaccattag
     2941 gcctatttag gtgacactat agaacaagtt tgtacaaaaa agcaggctgg taccggtccg
     3001 gaattcgcca ccatggatgt cggtgctctt gagagtttga ggggaaatgc agatttggct
     3061 tacatcctga gcatggagcc ctgtggccac tgcctcatta tcaacaatgt gaacttctgc
     3121 cgtgagtccg ggctccgcac ccgcactggc tccaacatcg actgtgagaa gttgcggcgt
     3181 cgcttctcct cgctgcattt catggtggag gtgaagggcg acctgactgc caagaaaatg
     3241 gtgctggctt tgctggagct ggcgcggcag gaccacggtg ctctggactg ctgcgtggtg
     3301 gtcattctct ctcacggctg tcaggccagc cacctgcagt tcccaggggc tgtctacggc
     3361 acagatggat gccctgtgtc ggtcgagaag attgtgaaca tcttcaatgg gaccagctgc
     3421 cccagcctgg gagggaagcc caagctcttt ttcatccagg cctgtggtgg ggagcagaaa
     3481 gaccatgggt ttgaggtggc ctccacttcc cctgaagacg agtcccctgg cagtaacccc
     3541 gagccagatg ccaccccgtt ccaggaaggt ttgaggacct tcgaccagct ggacgccata
     3601 tctagtttgc ccacacccag tgacatcttt gtgtcctact ctactttccc aggttttgtt
     3661 tcctggaggg accccaagag tggctcctgg tacgttgaga ccctggacga catctttgag
     3721 cagtgggctc actctgaaga cctgcagtcc ctcctgctta gggtcgctaa tgctgtttcg
     3781 gtgaaaggga tttataaaca gatgcctggt tgctttaatt tcctccggaa aaaacttttc
     3841 tttaaaacat cagggggcag tggcgggagc gggcgcgcct atccctacga tgtgcccgat
     3901 tacgctggtg gatccggcgg gtcagatgag atggaagagt gctctcagca cggcggatct
     3961 ggaggttcta caggctgcgt ggtcatagtg ggcaggatcg tcttgtccgg atccggcact
     4021 agtgcgccca tcacggcgta cgcccagcag acgagaggcc tcctagggtg tataatcacc
     4081 agcctgactg gccgggacaa aaaccaagtg gagggtgagg tccagatcat gtcaactgct
     4141 acccaaacct tcctggcaac gtgcatcaat ggggtatgct gggcagtcta ccacggggcc
     4201 ggaacgagga ccatcgcatc acccaagggt cctgtcatcc agatgtatac caatgtggac
     4261 caagaccttg tgggctggcc cgctcctcaa ggttcccgct cattgacacc ctgtacctgc
     4321 ggctcctcgg acctttacct ggtcacgagg cacgccgatg tcattcccgt gcgccggcga
     4381 ggtgatggca ggggtagcct gctttcgccc cggcccattt cctacttgaa aggctcctcg
     4441 gggggtccgc tgttgtgccc cgcgggacac gccgtgggcc tattcagggc cgcggtgtgc
     4501 acccgtggag tggctaaagc ggtggacttt atccctgtgg agaacctaga gacaaccatg
     4561 agatccccgg tgttcacgga caactcctct ccaccagcag tcaccctgac gcacgggggt
     4621 tcaggcggta gtgatgtcgg tgctcttgag agtttgaggg gaaatgcaga tttggcttac
     4681 atcctgagca tggagccctg tggccactgc ctcattatca acaatgtgaa cttctgccgt
     4741 gagtccgggc tccgcacccg cactggctcc aacatcgact gtgagaagtt gcggcgtcgc
     4801 ttctcctcgc tgcatttcat ggtggaggtg aagggcgacc tgactgccaa gaaaatggtg
     4861 ctggctttgc tggagctggc gcggcaggac cacggtgctc tggactgctg cgtggtggtc
     4921 attctctctc acggctgtca ggccagccac ctgcagttcc caggggctgt ctacggcaca
     4981 gatggatgcc ctgtgtcggt cgagaagatt gtgaacatct tcaatgggac cagctgcccc
     5041 agcctgggag ggaagcccaa gctctttttc atccaggcct gtggtgggga gcagaaagac
     5101 catgggtttg aggtggcctc cacttcccct gaagacgagt cccctggcag taaccccgag
     5161 ccagatgcca ccccgttcca ggaaggtttg aggaccttcg accagctgga cgccatatct
     5221 agtttgccca cacccagtga catctttgtg tcctactcta ctttcccagg ttttgtttcc
     5281 tggagggacc ccaagagtgg ctcctggtac gttgagaccc tggacgacat ctttgagcag
     5341 tgggctcact ctgaagacct gcagtccctc ctgcttaggg tcgctaatgc tgtttcggtg
     5401 aaagggattt ataaacagat gcctggttgc tttaatttcc tccggaaaaa acttttcttt
     5461 aaaacatcat agaagcttta cgcgtaccca gctttcttgt acaaagtggt ccctatagtg
     5521 agtcgtatta taagctaggc actggccgtc gttttacaac gtcgtgactg ggaaaactgc
     5581 tagcttggga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     5641 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     5701 ctagctgcat atgcttgctg cttgagagtt ttgcttactg agtatgattt atgaaaatat
     5761 tatacacagg agctagtgat tctaattgtt tgtgtatttt agattcacag tcccaaggct
     5821 catttcaggc ccctcagtcc tcacagtctg ttcatgatca taatcagcca taccacattt
     5881 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     5941 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     6001 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6061 tccaaactca tcaatgtatc ttatcatgtc tggatcgatc ctgcattaat gaatcggcca
     6121 acgcgcgggg agaggcggtt tgcgtattgg ctggcgtaat agcgaagagg cccgcaccga
     6181 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     6241 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6301 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6361 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6421 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6481 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6541 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6601 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6661 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6721 tttatttttc taaatacatt caaatatgta tccgctcatg ccaggtcttg gactggtgag
     6781 aacggcttgc tcggcagctt c
//
