LOCUS       Exported                7485 bp ds-DNA     circular SYN 14-MAY-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    P1794_7_ZZ-LTL-BICDR1-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 14, 2018 from SnapGene Viewer 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..26
                     /label=I-CeuI
                     /note="ApEinfo_fwdcolor=#fff811
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#fff811"
     misc_feature    232..255
                     /label=8xHis
     misc_feature    262..675
                     /label=ZZ
                     /note="ApEinfo_fwdcolor=#ff0080
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    676..765
                     /label=Linker TEV
                     /note="ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    766..2496
                     /label=BICDR1
                     /note="ApEinfo_fwdcolor=#0080ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    2497..3186
                     /label=GFP
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    3192..3199
                     /label=mutiple cloning site
                     /note="ApEinfo_fwdcolor=#8c96c6
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#8c96c6"
     polyA_signal    complement(3259..3450)
                     /label=SV40 late polyA
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink"
     misc_feature    complement(3516..3538)
                     /label=T7R (T7 terminator primer)
                     /note="ApEinfo_fwdcolor=#fff80a
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#fff80a"
     C_region        3574..3740
                     /label=Tn7L
                     /note="ApEinfo_fwdcolor=#08eaff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#08eaff
                     vntifkey=2"
     enhancer        3741..4452
                     /label=1629
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=9"
     misc_feature    complement(4461..5293)
                     /label=Ori
                     /note="ApEinfo_fwdcolor=#ff081d
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff081d
                     vntifkey=21"
     enhancer        5349..6313
                     /label=lef2-603
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=9"
     misc_feature    6338..6536
                     /label=Tn7R
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=cyan"
     CDS             complement(6626..7378)
                     /label=PromGen CDS
                     /label=PromGenCDS
                     /note="ApEinfo_fwdcolor=#827cb4
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#827cb4
                     vntifkey=4"
     misc_feature    complement(7448..7479)
                     /label=LoxP
                     /note="ApEinfo_fwdcolor=#ff081d
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff081d
                     vntifkey=21"
     misc_recomb     7449..7482
                     /label=LoxP(1)
                     /note="ApEinfo_fwdcolor=steel blue
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=LoxP
                     ApEinfo_revcolor=orange"
ORIGIN
        1 taactataac ggtcctaagg tagcgagttt aaacactagt atcgattcgc gacctactcc
       61 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
      121 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
      181 taccgtccca ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat
      241 caccatcacc atcacggttc tatgtgcatg gcaggccttg cgcaacacga tgaagccgtg
      301 gacaacaaat tcaacaaaga acaacaaaac gcgttctatg agatcttaca tttacctaac
      361 ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc
      421 gctaaccttt tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacaac
      481 aaattcaaca aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac
      541 gaagaacaac gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac
      601 cttttagcag aagctaaaaa gctaaatgat gctcaggcgc cgaaagtaga cgcaaattcc
      661 gcggggaagt caaccgatta tgatattcca actactgcta gcgagaacct atatttccag
      721 ggagattatg atatccctac gactgagaat ctttattttc agggcatgtc cgccttctgc
      781 ctgggcttgg ccggccgagc ttcagcaccc gccgagccgg acagcgcctg ctgcatggag
      841 ctgcccgctg gggccgggga cgcagtccgg agtcccgcca ctgctgccgc cctcgtctcc
      901 ttccccggag gtcccgggga actggaactg gccttagagg aggagctggc gttgctggcg
      961 gccggggagc ggtcgtccga gcccggcgag catcctcagg cggagccgga gtctccggtt
     1021 gaggggcacg ggccgccgct gccgccgccg cctacccagg atcccgagct gctgtcggtg
     1081 atccgccaga aggagaagga tctggtgttg gcggcccgac taggcaaggc gctgctcgag
     1141 aggaaccagg acatgagccg gcagtacgag cagatgcaca aggagctgac ggacaagcta
     1201 gagcacctag aacaagagaa acatgaactg agaaggcgat tcgagaatcg agaaggggag
     1261 tgggaaggac gggtgtctga gctggagacg gacgtgaagc agctacagga tgagttggag
     1321 aggcagcagc tccaccttcg ggaagcagac cgagaaaaaa cacgagctgt ccaggaactg
     1381 tcagaacaga accagaggct gctggaccaa ctcagcaggg catctgaagt tgagagacag
     1441 ctctccatgc aggtccatgc tctcaaagaa gacttccggg agaaaaattc ctccaccaac
     1501 cagcatatca tccggctgga gagtctgcag gccgagatca agatgctgtc agatcgaaaa
     1561 cgggagctgg agcatcggct cagcgccacc ttggaggaga atgacctgct tcaagggact
     1621 gtggaggagc tgcaggaccg ggtgctgatc ctagaaaggc agggccatga caaagaccta
     1681 cagctgcacc aaagccagct ggagctccag gaggtgcggc tgtcctaccg gcagctgcag
     1741 ggcaaggtgg aggaactcac ggaggagagg agtttgcaga gctcggctgc gaccagcacg
     1801 tccctgctgt cagagatcga gcagagcatg gaggccgagg agctggagca ggagagagag
     1861 cagctgaggc tgcagctctg ggaagcctac tgccaggttc gatatctctg ctcacacctc
     1921 cgagggaatg acagtgccga ctcagccgta tccacagact cctccatgga cgagtcctcg
     1981 gagacctcgt cggccaagga cgtgcctgcc ggcagcctgc gcactgccct caacgatctc
     2041 aagaggctga tacagagcat cgtggacggt gtggagccca cggtgacact gctgagcgtg
     2101 gagatgaccg cactgaagga ggaaagggac cgactcagag tgacctctga ggacaaggag
     2161 ccaaaggagc agcttcagaa ggccatcagg gaccgggacg aggccattgc caagaagaac
     2221 gctgtggagc tggaactcgc caagtgcaag atggatatga tgtctctgaa cagccagcta
     2281 ctggatgcca ttcagcaaaa actgaacctc tctcagcagc tggaggcttg gcaggatgac
     2341 atgcacaggg tcattgacag gcagctgatg gacacgcacc tgaaggaaca gagccggcct
     2401 gctgctgctg ccttccctag gggccacggc gtagggcggg ggcaggaacc cagcaccgca
     2461 gatggcaaac ggctcttctc cttcttcagg aagattatgt ctaaaggtga agaattattc
     2521 actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct
     2581 gtctccggtg aaggtgaagg tgatgctact tacggtaaat tgaccttaaa atttatttgt
     2641 actactggta aattgccagt tccatggcca accttagtca ctactttcgg ttatggtgtt
     2701 caatgttttg cgagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg
     2761 ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc
     2821 agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt aaaaggtatt
     2881 gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta taactctcac
     2941 aatgtttaca tcatggctga caaacaaaag aatggtatca aagttaactt caaaattaga
     3001 cacaacattg aagacggttc tgttcaatta gctgaccatt atcaacaaaa tactccaatt
     3061 ggtgatggtc cagtcttgtt accagacaac cattacttat ccactcaatc tgccttatcc
     3121 aaggatccaa acgaaaagag agaccacatg gtcttgttag aatttgttac tgctgctggt
     3181 attacctaat gagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt
     3241 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     3301 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3361 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3421 caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctagaagatc
     3481 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
     3541 tatcataacc cctagggtat acccatctaa ttggaaccag ataagtgaaa tctagttcca
     3601 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
     3661 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
     3721 aactattttg tccgcccaca accggtggag gaaattctcc ttgaagtttc cctggtgttc
     3781 aaagtaaagg agtttgcacc agacgcacct ctgttcactg gtccggcgta ttaaaacacg
     3841 atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac
     3901 aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga
     3961 gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata
     4021 gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct
     4081 ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat
     4141 ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa
     4201 atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta
     4261 aacacgttaa atagagcttg gacatattta acatcgggcg tgttagcttt attaggccga
     4321 ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata
     4381 gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag
     4441 ctttttggaa ttaccggttg acttgggtca actgtcagac caagtttact catatatact
     4501 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4561 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4621 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4681 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4741 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4801 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4861 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4921 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4981 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5041 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5101 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5161 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5221 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5281 tgctcacatg ttctttcctg cgttatcccc tgattgactt gggtcgctct tcctgtggat
     5341 gcgcaggtat gtacaggaag aggtttatac taaactgtta cattgcaaac gtggtttcgt
     5401 gtgccaagtg tgaaaaccga tgtttaatca aggctctgac gcatttctac aaccacgact
     5461 ctaagtgtgt gggtgaagtc atgcatcttt taatcaaatc ccaagatgtg tataaaccac
     5521 caaactgcca aaaaatgaaa actgtcgaca agctctgtcc gtttgctggc aactgcaagg
     5581 gtctcaatcc tatttgtaat tattgaataa taaaacaatt ataaatgtca aatttgtttt
     5641 ttattaacga tacaaaccaa acgcaacaag aacatttgta gtattatcta taattgaaaa
     5701 cgcgtagtta taatcgctga ggtaatattt aaaatcattt tcaaatgatt cacagttaat
     5761 ttgcgacaat ataattttat tttcacataa actagacgcc ttgtcgtctt cttcttcgta
     5821 ttccttctct ttttcatttt tctcttcata aaaattaaca tagttattat cgtatccata
     5881 tatgtatcta tcgtatagag taaatttttt gttgtcataa atatatatgt cttttttaat
     5941 ggggtgtata gtaccgctgc gcatagtttt tctgtaattt acaacagtgc tattttctgg
     6001 tagttcttcg gagtgtgttg ctttaattat taaatttata taatcaatga atttgggatc
     6061 gtcggttttg tacaatatgt tgccggcata gtacgcagct tcttctagtt caattacacc
     6121 attttttagc agcaccggat taacataact ttccaaaatg ttgtacgaac cgttaaacaa
     6181 aaacagttca cctccctttt ctatactatt gtctgcgagc agttgtttgt tgttaaaaat
     6241 aacagccatt gtaatgagac gcacaaacta atatcacaaa ctggaaatgt ctatcaatat
     6301 atagttgctg attgcgcaga tgccctgcgt aagcgggtgt gggcggacaa taaagtctta
     6361 aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca gaatagttgt
     6421 aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt tatggctaaa
     6481 gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa
     6541 gggcatgtaa agactatatt cgcggcgttg tgacaattta ccgaacaact ccgcggccgg
     6601 gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg
     6661 catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta
     6721 atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct tgaggagatt
     6781 gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatagat
     6841 atagatctca ctacgcggct gctcaaactt gggcagaacg taagccgcga gagcgccaac
     6901 aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga gcaagttccc
     6961 gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga actcacgacc
     7021 gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc tacatgtgcg
     7081 aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct gctgcgtaac
     7141 atcgttgctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     7201 aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag tcataacaag
     7261 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
     7321 tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct tatgtcaact
     7381 gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg
     7441 aagtcgccat aacttcgtat agcatacatt atacgaagtt atctg
//
