LOCUS              pRibo_APEX2        5136 bp ds-DNA   circular     18-JUN-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     pRibo mpt63-APEX2 from 1 to 5226
            
            
            
            
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    276..282
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=RBS
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=green
     misc_feature    233..270
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=aptamer
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=green
     misc_feature    3901..5110
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=oriM
                     /ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_revcolor=green
     misc_feature    2463..3249
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=oriE
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    1360..2175
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=kanR (aph)
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=green
     misc_feature    join(299..736,738..1090)
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=APEX
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=green
     misc_feature    299..340
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=V5 epitope
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     misc_feature    377..379
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=K14D mutation
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    458..460
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=W41F mutation
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    671..673
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=E112K mutation
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    738..739
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=To make APEX2, mutate Ala150Pro (GCC to CCC)
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
ORIGIN
        1 TCTAGAGGTG aCCACAACGC GCCCGCTTTG ATCGGGGACG TCTGCGGCCG ACCATTTACG
       61 GGTCTTGTTG TCGTTGGCGG TCATGGGCCG AACATACTCA CCCGGATCGG AGGGCCGAGG
      121 ACAAGGTCGA ACGAGGGGCA TGACCCGGTG CGGGGCTTCT TGCACTCGGC ATAGGCGAGT
      181 GCTAAGAATA ACGTTGGCAC TCGCGACCGG GATACGACTC ACTATAGGTA CCGGTGATAC
      241 CAGCATCGTC TTGATGCCCT TGGCAGCACC CTGCTAAGGA GGCAACAAGA TGcatatgGG
      301 CAAGCCCATC CCCAACCCCC TGCTGGGCCT GGACAGCACC GGAAAGTCTT ACCCAACTGT
      361 GAGTGCTGAT TACCAGGACG CCGTTGAGAA GGCGAAGAAG AAGCTCAGAG GCTTCATCGC
      421 TGAGAAGAGA TGCGCTCCTC TAATGCTCCG TTTGGCATTC CACTCTGCTG GAACCTTTGA
      481 CAAGGGCACG AAGACCGGTG GACCCTTCGG AACCATCAAG CACCCTGCCG AACTGGCTCA
      541 CAGCGCTAAC AACGGTCTTG ACATCGCTGT TAGGCTTTTG GAGCCACTCA AGGCGGAGTT
      601 CCCTATTTTG AGCTACGCCG ATTTCTACCA GTTGGCTGGC GTTGTTGCCG TTGAGGTCAC
      661 GGGTGGACCT AAGGTTCCAT TCCACCCTGG AAGAGAGGAC AAGCCTGAGC CACCACCAGA
      721 GGGTCGCTTG CCCGATCCCA CTAAGGGTTC TGACCATTTG AGAGATGTGT TTGGCAAAGC
      781 TATGGGGCTT ACTGACCAAG ATATCGTTGC TCTATCTGGG GGTCACACTA TTGGAGCTGC
      841 ACACAAGGAG CGTTCTGGAT TTGAGGGTCC CTGGACCTCT AATCCTCTTA TTTTCGACAA
      901 CTCATACTTC ACGGAGTTGT TGAGTGGTGA GAAGGAAGGT CTCCTTCAGC TACCTTCTGA
      961 CAAGGCTCTT TTGTCTGACC CTGTATTCCG CCCTCTCGTT GATAAATATG CAGCGGACGA
     1021 AGATGCCTTC TTTGCTGATT ACGCTGAGGC TCACCAAAAG CTTTCCGAGC TTGGGTTTGC
     1081 TGATGCCTAA TGAagatgga gaccAAGCTT ATCGATGTCG ACGTAGTTAA CTAGCGTACG
     1141 ATCGACTGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT
     1201 TATCTGTTGT TTGTCCGGCC ATCATGGCCG CGGTGATCAG CTAGCCAACA AAGCGACGTT
     1261 GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA ATATATCATC ATGAACAATA
     1321 AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA
     1381 ACGTCTTGCT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA
     1441 TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC
     1501 CATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT
     1561 GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT
     1621 ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGGAA AACAGCATTC
     1681 CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC
     1741 CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT
     1801 CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT
     1861 GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA TCTTTTGCCA
     1921 TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC
     1981 GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG
     2041 GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT
     2101 TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC
     2161 GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG
     2221 ACTTGACGGG ACGGCGGCTT TGTTGAATAA ATCGAACTTT TGCTGAGTTG AAGGATCAGA
     2281 TCACGCATCT TCCCGACAAC GCAGACCGTT CCGTGGCAAA GCAAAAGTTC AAAATCACCA
     2341 ACTGGTCCAC CTACAACAAA GCTCTCATCA ACCGTGGCTC CCTCACTTTC TGGCTGGATG
     2401 ATGGGGCGAT TCAGGCCTGG TATGAGTCAG CAACACCTTC TTCACGAGGC AGACCTCACT
     2461 AGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT
     2521 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT
     2581 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA
     2641 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG
     2701 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA
     2761 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG
     2821 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA
     2881 GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA
     2941 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA
     3001 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT
     3061 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC
     3121 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT
     3181 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA
     3241 CCGAGCGCAA CGCGTGAGCC CACCAGCTCC GTAAGTTCGG GTGCTGTGTG GCTCGTACCC
     3301 GCGCATTCAG GCGGCAGGGG GTCTAACGGG TCTAAGGCGG CGTGTACGGC CGCCACAGCG
     3361 GCTCTTAGCG GCCCGGAAAC GTCCTCGAAA CGACGCATGT GTTCCTCCTG GTTGGTACAG
     3421 GTGGTTGGGG GTGCTCGGCT GTCGCTGGTG TTTCATCATC AGGGCTCGAC GGGAGAGCGG
     3481 GGGAGTGTGC AGTTGTGGGG TGGCCCCTCA GCGAAATATC TGACTTGGAG CTCGTGTCGG
     3541 ACCATACACC GGTGATTAAT CGTGGTTTAT TATCAAGCGT GAGCCACGTC GCCGACGAAT
     3601 TTGAGCAGCT CTGGCTGCCG TACTGGTCCC TGGCAAGCGA CGATCTGCTC GAGGGGATCT
     3661 ACCGCCAAAG CCGCGCGTCG GCCCTAGGCC GCCGGTACAT CGAGGCGAAC CCAACAGCGC
     3721 TGGCAAACCT GCTGGTCGTG GACGTAGACC ATCCAGACGC AGCGCTCCGA GCGCTCAGCG
     3781 CCCGGGGGTC CCATCCGCTG CCCAACGCGA TCGTGGGCAA TCGCGCCAAC GGCCACGCAC
     3841 ACGCAGTGTG GGCACTCAAC GCCCCTGTTC CACGCACCGA ATACGCGCGG CGTAAGCCGC
     3901 GCCGTCGATG GCGACCGCAG TTACTCAGGC CTCATGACCA AAAACCCCGG CCACATCGCC
     3961 TGGGAAACGG AATGGCTCCA CTCAGATCTC TACACACTCA GCCACATCGA GGCCGAGCTC
     4021 GGCGCGAACA TGCCACCGCC GCGCTGGCGT CAGCAGACCA CGTACAAAGC GGCTCCGACG
     4081 CCGCTAGGGC GGAATTGCGC ACTGTTCGAT TCCGTCAGGT TGTGGGCCTA TCTTCCCGCC
     4141 CTCATGCGGA TCTACCTGCC GACCCGGAAC GTGGACGGAC TCGGCCGCGC GATCTATGCC
     4201 GAGTGCCACG CGCGAAACGC CGAATTTCCG TGCAACGACG TGTGTCCCGG ACCGCTACCG
     4261 GACAGCGAGG TCCGCGCCAT CGCCAACAGC ATTTGGCGTT GGATCACAAC CAAGTCGCGC
     4321 ATTTGGGCGG ACGGGATCGT GGTCTACGAG GCCACACTCA GTGCGCGCCA TGCGGCCATC
     4381 TCGCGGAAGG GCGCAGCAGC GCGCACGGCG GCGAGCACAG TTGCGCGGCG CGCAAAGTCC
     4441 GCGTCAGCCA TGGAGGCATT GCTATGAGCG ACGGCTACAG CGACGGCTAC AGCGACGGCT
     4501 ACAACTGGCA GCCGACTGTC CGCAAAAAGC GGCGCGTGAC CGCCGCCGAA GGCGCTCGAA
     4561 TCACCGGACT ATCCGAACGC CACGTCGTCC GGCTCGTGGC GCAGGAACGC AGCGAGTGGT
     4621 TCGCCGAGCA GGCTGCACGC CGCGAACGCA TCCGCGCCTA TCACGACGAC GAGGGCCACT
     4681 CTTGGCCGCA AACGGCCAAA CATTTCGGGC TGCATCTGGA CACCGTTAAG CGACTCGGCT
     4741 ATCGGGCGAG GAAAGAGCGT GCGGCAGAAC AGGAAGCGGC TCAAAAGGCC CACAACGAAG
     4801 CCGACAATCC ACCGCTGTTC TAACGCAATT GGGGAGCGGG TGTCGCGGGG GTTCCGTGGG
     4861 GGGTTCCGTT GCAACGGGTC GGACAGGTAA AAGTCCTGGT AGACGCTAGT TTTCTGGTTT
     4921 GGGCCATGCC TGTCTCGTTG CGTGTTTCGT TGCGTCCGTT TTGAATACCA GCCAGACGAG
     4981 ACGGGGTTCT ACGAATCTTG GTCGATACCA AGCCATTTCC GCTGAATATC GTGGAGCTCA
     5041 CCGCCAGAAT CGGTGGTTGT GGTGATGTAC GTGGCGAACT CCGTTGTAGT GCTTGTGGTG
     5101 GCATCCGTGG CGCGGCCGCG GTACCAGATC TTTAAA  
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