LOCUS         pRiboI_Sec_APEX2        5142 bp ds-DNA   circular     18-JUN-2018
DEFINITION  integrating mycobacterial complementation vector.
SOURCE      
  ORGANISM  
COMMENT     pMV306-pRibo-APEX2 from 1 to 5052
            
            
            
            
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             1934..2955
                     /vntifkey="4"
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=int
                     /ApEinfo_fwdcolor=#8000ff
                     /ApEinfo_revcolor=#8000ff
     misc_marker     3222..4037
                     /vntifkey="22"
                     /note="aph"
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=Kan
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=pink
     CDS             4301..4701
                     /vntifkey="4"
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=oriE
                     /ApEinfo_fwdcolor=#8000ff
                     /ApEinfo_revcolor=#8000ff
     misc_feature    276..282
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=RBS
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=green
     misc_feature    233..270
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=aptamer
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=green
     misc_feature    548..550
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=W41F mutation
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    761..763
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=E112K mutation
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    join(389..826,828..1180)
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=APEX
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=green
     misc_feature    389..430
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=V5 epitope
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     misc_feature    467..469
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=K14D mutation
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     CDS             1607..1676
                     /vntifkey="4"
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=attP
                     /ApEinfo_fwdcolor=#8000ff
                     /ApEinfo_revcolor=#8000ff
     misc_feature    299..382
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=mpt63 signal peptide
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=green
     misc_feature    828..829
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=To make APEX2, mutate Ala150Pro (GCC to CCC)
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
ORIGIN
        1 TCTAGAGGTG aCCACAACGC GCCCGCTTTG ATCGGGGACG TCTGCGGCCG ACCATTTACG
       61 GGTCTTGTTG TCGTTGGCGG TCATGGGCCG AACATACTCA CCCGGATCGG AGGGCCGAGG
      121 ACAAGGTCGA ACGAGGGGCA TGACCCGGTG CGGGGCTTCT TGCACTCGGC ATAGGCGAGT
      181 GCTAAGAATA ACGTTGGCAC TCGCGACCGG GATACGACTC ACTATAGGTA CCGGTGATAC
      241 CAGCATCGTC TTGATGCCCT TGGCAGCACC CTGCTAAGGA GGCAACAAGA TGcatatgAA
      301 GCTCACCACA ATGATCAAGA CGGCAGTAGC GGTCGTGGCC ATGGCGGCCA TCGCGACCTT
      361 TGCGGCACCG GTCGCGTTGG CTggatccGG CAAGCCCATC CCCAACCCCC TGCTGGGCCT
      421 GGACAGCACC GGAAAGTCTT ACCCAACTGT GAGTGCTGAT TACCAGGACG CCGTTGAGAA
      481 GGCGAAGAAG AAGCTCAGAG GCTTCATCGC TGAGAAGAGA TGCGCTCCTC TAATGCTCCG
      541 TTTGGCATTC CACTCTGCTG GAACCTTTGA CAAGGGCACG AAGACCGGTG GACCCTTCGG
      601 AACCATCAAG CACCCTGCCG AACTGGCTCA CAGCGCTAAC AACGGTCTTG ACATCGCTGT
      661 TAGGCTTTTG GAGCCACTCA AGGCGGAGTT CCCTATTTTG AGCTACGCCG ATTTCTACCA
      721 GTTGGCTGGC GTTGTTGCCG TTGAGGTCAC GGGTGGACCT AAGGTTCCAT TCCACCCTGG
      781 AAGAGAGGAC AAGCCTGAGC CACCACCAGA GGGTCGCTTG CCCGATCCCA CTAAGGGTTC
      841 TGACCATTTG AGAGATGTGT TTGGCAAAGC TATGGGGCTT ACTGACCAAG ATATCGTTGC
      901 TCTATCTGGG GGTCACACTA TTGGAGCTGC ACACAAGGAG CGTTCTGGAT TTGAGGGTCC
      961 CTGGACCTCT AATCCTCTTA TTTTCGACAA CTCATACTTC ACGGAGTTGT TGAGTGGTGA
     1021 GAAGGAAGGT CTCCTTCAGC TACCTTCTGA CAAGGCTCTT TTGTCTGACC CTGTATTCCG
     1081 CCCTCTCGTT GATAAATATG CAGCGGACGA AGATGCCTTC TTTGCTGATT ACGCTGAGGC
     1141 TCACCAAAAG CTTTCCGAGC TTGGGTTTGC TGATGCCTAA TGAagatgga gaccAAGCTT
     1201 ATCGATGTCG ACGTAGTTAA CTAGCGTACG ATCGACTGCC AGGCATCAAA TAAAACGAAA
     1261 GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATGCCATCA TGGCCGCGGT GATCAGCTAG
     1321 AGCCGTGAAC GACAGGGCGA ACGCCAGCCC GCCGACGGCG AGGGTTCCGA CCGCTGCAAC
     1381 TCCCGGTGCA ACCTTGTCCC GGTCTATTCT CTTCACTGCA CCAGCTCCAA TCTGGTGTGA
     1441 ATGCCCCTCG TCTGTTCGCG CAGGCGGGGG GCTCTATTCG TTTGTCAGCA TCGAAAGTAG
     1501 CCAGATCAGG GATGCGTTGC AACCGCGTAT GCCCAGGTCA GAAGAGTCGC ACAAGAGTTG
     1561 CAGACCCCTG GAAAGAAAAA TGGCCAGAGG GCGAAAACAC CCTCTGACCA GCGGAGCGGG
     1621 CGACGGGAAT CGAACCCGCG TAGCTAGTTT GGAAGAATGG GTGTCTGCCG ACCACATATA
     1681 TGGGCCGGTC AAGATAGGTT TTTACCCCCT CTCGGCTGCA TCCTCTAAGT GGAAAGAAAT
     1741 TGCAGGTCGT AGAAGCGCGT TGAAGCCTGA GAGTTGCACA GGAGTTGCAA CCCGGTAGCC
     1801 TTGTTCACGA CGAGAGGAGA CCTAGTTGGC ACGTCGCGGA TGGGGATCGC TGAAGACTCA
     1861 GCGCAGCGGG AGGATCCAAG CCTCATACGT CAACCCGCAG GACGGTGTGA GGTACTACGC
     1921 GCTGCAGACC TACGACAACA AGATGGACGC CGAAGCCTGG CTCGCGGGCG AGAAGCGGCT
     1981 CATCGAGATG GAGACCTGGA CCCCTCCACA GGACCGGGCG AAGAAGGCAG CCGCCAGCGC
     2041 CATCACGCTG GAGGAGTACA CCCGGAAGTG GCTCGTGGAG CGCGACCTCG CAGACGGCAC
     2101 CAGGGATCTG TACAGCGGGC ACGCGGAGCG CCGCATCTAC CCGGTGCTAG GTGAAGTGGC
     2161 GGTCACAGAG ATGACGCCAG CTCTGGTGCG TGCGTGGTGG GCCGGGATGG GTAGGAAGCA
     2221 CCCGACTGCC CGCCGGCATG CCTACAACGT CCTCCGGGCG GTGATGAACA CAGCGGTCGA
     2281 GGACAAGCTG ATCGCAGAGA ACCCGTGCCG GATCGAGCAG AAGGCAGCCG ATGAGCGCGA
     2341 CGTAGAGGCG CTGACGCCTG AGGAGCTGGA CATCGTCGCC GCTGAGATCT TCGAGCACTA
     2401 CCGGATCGCG GCATACATCC TGGCGTGGAC GAGCCTCCGG TTCGGAGAGC TGATCGAGCT
     2461 TCGCCGCAAG GACATCGTGG ACGACGGCAT GACGATGAAG CTCCGGGTGC GCCGTGGCGC
     2521 TTCCCGCGTG GGGAACAAGA TCGTCGTTGG CAACGCCAAG ACCGTCCGGT CGAAGCGTCC
     2581 TGTGACGGTT CCGCCTCACG TCGCGGAGAT GATCCGAGCG CACATGAAGG ACCGTACGAA
     2641 GATGAACAAG GGCCCCGAGG CATTCCTGGT GACCACGACG CAGGGCAACC GGCTGTCGAA
     2701 GTCCGCGTTC ACCAAGTCGC TGAAGCGTGG CTACGCCAAG ATCGGTCGGC CGGAACTCCG
     2761 CATCCACGAC CTCCGCGCTG TCGGCGCTAC GTTCGCCGCT CAGGCAGGTG CGACGACCAA
     2821 GGAGCTGATG GCCCGTCTCG GTCACACGAC TCCTAGGATG GCGATGAAGT ACCAGATGGC
     2881 GTCTGAGGCC CGCGACGAGG CTATCGCTGA GGCGATGTCC AAGCTGGCCA AGACCTCCTG
     2941 AAACGCAAAA AGCCCCCCTC CCAAGGACAC TGAGTCCTAA AGAGGGGGGT TTCTTGTCAG
     3001 TACGCGAAGA ACCACGCCTG GCCGCGAGCG CCAGCACCGC CGCTCTGTGC GGAGACCTGG
     3061 GCACCAGCCC CGCCGCCGCC AGGAGCATTG CCGTTCCCGC CAGCTAGCAA CAAAGCGACG
     3121 TTGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA AAATATATCA TCATGAACAA
     3181 TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG
     3241 AAACGTCTTG CTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA
     3301 AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC
     3361 CCCATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG
     3421 ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT
     3481 TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGG AAAACAGCAT
     3541 TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT
     3601 TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT
     3661 TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG
     3721 ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AATCTTTTGC
     3781 CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG
     3841 ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC
     3901 AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC
     3961 TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC
     4021 TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC
     4081 TGACTTGACG GGACGGCGGC TTTGTTGAAT AAATCGAACT TTTGCTGAGT TGAAGGATCA
     4141 GATCACGCAT CTTCCCGACA ACGCAGACCG TTCCGTGGCA AAGCAAAAGT TCAAAATCAC
     4201 CAACTGGTCC ACCTACAACA AAGCTCTCAT CAACCGTGGC TCCCTCACTT TCTGGCTGGA
     4261 TGATGGGGCG ATTCAGGCCT GGTATGAGTC AGCAACACCT TCTTCACGAG GCAGACCTCA
     4321 CTAGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
     4381 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
     4441 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
     4501 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
     4561 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
     4621 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
     4681 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
     4741 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
     4801 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
     4861 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
     4921 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
     4981 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA
     5041 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC
     5101 GACCGAGCGC AACGCGTGCG GCCGCGGTAC CAGATCTTTA AA 
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