LOCUS       pcDNA3.1-tCRMod-GGSGGS7 6914 bp ds-DNA     circular     07-MAY-2018
DEFINITION  .
KEYWORDS    "creator:Matthew Berginski" "marker:NeoR/KanR, AmpR"               
FEATURES             Location/Qualifiers
     CDS             complement(5921..6580)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1607..1732
                     /label="(GGSGGS)7 extensible domain"
                     /ApEinfo_revcolor=#9f1fc0
                     /ApEinfo_fwdcolor=#9f1fc0
     misc_binding    complement(4782..4804)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3406..3483
                     /label="SV40 ORI"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     terminator      2511..2738
                     /label="bGH PA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        complement(6820..6848)
                     /label="Amp prom"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     promoter        complement(4809..4838)
                     /label="lac"
                     /ApEinfo_revcolor=#4682b4
                     /ApEinfo_fwdcolor=#4682b4
     CDS             3625..4416
                     /label="Kan/neoR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     863..882
                     /label="T7"
                     /ApEinfo_revcolor=#008b8b
                     /ApEinfo_fwdcolor=#008b8b
     CDS             923..1606
                     /label=""Minimal" Clover (residues 1 - 228)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(4756..4776)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#008b8b
                     /ApEinfo_fwdcolor=#008b8b
     CDS             1733..2434
                     /label=""Minimal" mRuby2 (residues 3 - 236)"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
ORIGIN
        1 Gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttaccA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC
      961 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTCC GCGGCGAGGG
     1021 CGAGGGCGAT GCCACCAACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT
     1081 GCCCGTGCCC TGGCCCACCC TCGTGACCAC CTTCGGCTAC GGCGTGGCCT GCTTCAGCCG
     1141 CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT
     1201 CCAGGAGCGC ACCATCTCTT TCAAGGACGA CGGTACCTAC AAGACCCGCG CCGAGGTGAA
     1261 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA
     1321 CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTTCAAC AGCCACAACG TCTATATCAC
     1381 GGCCGACAAG CAGAAGAACG GCATCAAGGC TAACTTCAAG ATCCGCCACA ACGTTGAGGA
     1441 CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT
     1501 GCTGCTGCCC GACAACCACT ACCTGAGCCA TCAGTCCGCC CTGAGCAAAG ACCCCAACGA
     1561 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGggtg gatccggtgg
     1621 atcaggtgga tccggtggta gtggtggatc cggaggttct ggtggatccg gtggttcagg
     1681 tggatccggt ggatctggtg gatccggtgg tagtggtgga tccggaggta gcAAGGGCGA
     1741 AGAGCTGATC AAGGAAAATA TGCGTATGAA GGTGGTCATG GAAGGTTCGG TCAACGGCCA
     1801 CCAATTCAAA TGCACAGGTG AAGGAGAAGG CAATCCGTAC ATGGGAACTC AAACCATGAG
     1861 GATCAAAGTC ATCGAGGGAG GACCCCTGCC ATTTGCCTTT GACATTCTTG CCACGTCGTT
     1921 CATGTATGGC AGCCGTACTT TTATCAAGTA CCCGAAAGGC ATTCCTGATT TCTTTAAACA
     1981 GTCCTTTCCT GAGGGTTTTA CTTGGGAAAG AGTTACGAGA TACGAAGATG GTGGAGTCGT
     2041 CACCGTCATG CAGGACACCA GCCTTGAGGA TGGCTGTCTC GTTTACCACG TCCAAGTCAG
     2101 AGGGGTAAAC TTTCCCTCCA ATGGTCCCGT GATGCAGAAG AAGACCAAGG GTTGGGAGCC
     2161 TAATACAGAG ATGATGTATC CAGCAGATGG TGGTCTGAGG GGATACACTC ATATGGCACT
     2221 GAAAGTTGAT GGTGGTGGCC ATCTGTCTTG CTCTTTCGTA ACAACTTACA GGTCAAAAAA
     2281 GACCGTCGGG AACATCAAGA TGCCCGGTAT CCATGCCGTT GATCACCGCC TGGAAAGGTT
     2341 AGAGGAAAGT GACAATGAAA TGTTCGTAGT ACAACGCGAA CACGCAGTTG CCAAGTTCGC
     2401 CGGGCTTGGT GGTGGGATGG ACGAGCTGTA CAAGTAAGAA TTCTgcAGAt atCCagcaca
     2461 gtggcggccg ctcgagtcta gagggccCgt ttaaacccgc tgatcagcct cgactgtgcc
     2521 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     2581 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     2641 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2701 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     2761 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     2821 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     2881 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     2941 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3001 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3061 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3121 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3181 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     3241 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3301 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3361 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     3421 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     3481 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     3541 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     3601 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     3661 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     3721 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     3781 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     3841 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     3901 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     3961 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4021 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4081 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4141 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     4201 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4261 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     4321 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     4381 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     4441 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     4501 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     4561 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     4621 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4681 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     4741 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     4801 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     4861 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     4921 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4981 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5041 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5101 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5161 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5221 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5341 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5401 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5461 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5521 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5581 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5641 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
     5701 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5761 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5821 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6901 tgccacctga cgtc
//