LOCUS       Exported                8166 bp ds-DNA     circular SYN 27-AUG-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pEUI(+)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2018 from SnapGene 4.2.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        10..339
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      190..325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             432..1031
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     polyA_signal    1261..1395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        2172..2190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     2216..2232
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    2251..2495
                     /label=10X UAS
                     /note="color: #ff00ff"
     promoter        2571..2589
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     polyA_signal    2703..2837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        complement(2950..2968)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        3106..3210
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             3211..4071
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 3211 .. 3279 / #ccffcc / signal sequence
                        2: 3280 .. 4071 / #ccffcc
                     Cleavage site after base 3279"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4242..4830
                     /direction=RIGHT
                     /label=pUC ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     enhancer        5117..5496
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        5497..5700
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        5745..5763
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    5783..7309
                     /label=GAL4-VP16-EcR
                     /note="color: #ff0000"
     promoter        complement(7325..7343)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    7369..7593
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      7639..8067
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttgcatg cctgcaggtc
      361 ggccgccacg accggtgccg ccaccatccc ctgacccacg cccctgaccc ctcacaagga
      421 gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
      481 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
      541 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
      601 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
      661 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
      721 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
      781 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
      841 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
      901 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
      961 ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac ctggtgcatg acccgcaagc
     1021 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     1081 atggctccga ccgaagccga cccgggcggc cccgccgacc ccgcacccgc ccccgaggcc
     1141 caccgactct agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     1201 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     1261 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     1321 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     1381 tatcatgtct ggatccccag gaagctcctc tgtgtcctca taaaccctaa cctcctctac
     1441 ttgagaggac attccaatca taggctgccc atccaccctc tgtgtcctcc tgttaattag
     1501 gtcacttaac aaaaaggaaa ttgggtaggg gtttttcaca gaccgctttc taagggtaat
     1561 tttaaaatat ctgggaagtc ccttccactg ctgtgttcca gaagtgttgg taaacagccc
     1621 acaaatgtca acagcagaaa catacaagct gtcagctttg cacaagggcc caacaccctg
     1681 ctcatcaaga agcactgtgg ttgctgtgtt agtaatgtgc aaaacaggag gcacattttc
     1741 cccacctgtg taggttccaa aatatctagt gttttcattt ttacttggat caggaaccca
     1801 gcactccact ggataagcat tatccttatc caaaacagcc ttgtggtcag tgttcatctg
     1861 ctgactgtca actgtagcat tttttggggt tacagtttga gcaggatatt tggtcctgta
     1921 gtttgctaac acaccctgca gctccaaagg ttccccacca acagcaaaaa aatgaaaatt
     1981 tgacccttga atgggttttc cagcaccatt ttcatgagtt ttttgtgtcc ctgaatgcaa
     2041 gtttaacata gcagttaccc caataacctc agttttaaca gtaacagctt cccacatcaa
     2101 aatatttcca caggttaagt cctcatttaa attaggcaaa ggaattgctc aactttgtat
     2161 agaaaagttg gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg
     2221 tcgacggtat cgataagctt aggcctccaa ggcggagtac tgtcctccgg gctggcggag
     2281 tactgtcctc cggcaaggtc ggagtactgt cctccgacac tagaggtcgg agtactgtcc
     2341 tccgacgcaa ggcggagtac tgtcctccgg gctgcggagt actgtcctcc ggcaaggtcg
     2401 gagtactgtc ctccgacact agaggtcgga gtactgtcct ccgacgcaag gcggagtact
     2461 gtcctccggg ctggcggagt actgtcctcc ggcaagggtc gactctagag ggtatataat
     2521 ggatcccatc gcgtctcagc ctcactttga gctcctccac acggagcttg atttaggtga
     2581 cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgattcg aattcacgtg
     2641 ctagcgctta agaaattcaa ggcctctcga gcctctagaa ctatagtgag tcgtattacg
     2701 tagatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     2761 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     2821 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     2881 aggtgtggga ggttttttaa ttcgcggccg cggcgccaat gcattgggcc cggtacccag
     2941 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     3001 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3061 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     3481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     3661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3721 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     3781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     5041 catctgtgcg gtatttcaca ccgcatagtt aggcgttttg cgctgcttcg cgatgtacgg
     5101 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     5161 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     5221 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     5281 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     5341 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     5401 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     5461 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     5521 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     5581 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     5641 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     5701 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
     5761 agggagaccc aagcttatcg atatgaagct acagtcttct atcgaacaag catgcgatat
     5821 ttgccgactt aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa
     5881 gaacaactgg gagtgtcgct actctcccaa aaccaaaagg tctccgctga ctagggcaca
     5941 tctgacagaa gtggaatcaa ggctagaaag actggaacag ctatttctac tgatttttcc
     6001 tcgagaagac cttgacatga ttttgaaaat ggattcttta caggatataa aagcattgtt
     6061 aacaggatta tttgtacaag ataatgtgaa taaagatgcc gtcacagata gattggcttc
     6121 agtggagact gatatgcctc taacattgag acagcataga ataagtgcga catcatcatc
     6181 ggaagagagt agtaacaaag gtcaaagaca gttgactgta gagttcctgg atatggccga
     6241 cttcgagttt gagcagatgt ttaccgatgc ccttggaatt gacgagtacg gtgggctgca
     6301 gatgaggcct gaatgtgtca tacaggagcc cagtaaaaat aaagacaggc aaagacaaaa
     6361 gaaagacaaa ggaatattat tacctgttag tacgaccaca gtcgaagacc acatgccccc
     6421 gatcatgcaa tgtgatccac ctccgcccga ggccgccagg attcacgaag tcgtcccgag
     6481 gtatctttcg gagaagctga tggagcagaa caggcagaag aacataccac cattgtcggc
     6541 gaatcagaag tctctgatcg cgaggctcgt gtggtaccag gagggatatg agcagccctc
     6601 cgacgaggat ctcaaaagag taacgcagac ttggcagtcg gatgaagagg acgaggaatc
     6661 cgatctaccc ttccgccaga tcacggagat gacgatctta acggtccagt tgatcgtcga
     6721 gttcgccaag ggtctaccgg gcttttcgaa gatatcacag tctgatcaaa tcaccttatt
     6781 aaaagcctcg tccagcgagg tgatgatgct gcgggtggcg aggcgatacg acgccgcgtc
     6841 cgacagcatt ctgttcgcca acaacaaggc gtacacgcgc gacaactacc gcaaggcggg
     6901 catggccgag gtcatcgaag acctcctaca cttctgccgg tgcatgttcg cgatgggcat
     6961 ggacaatgtg cactttgcac tgctcacggc catcgttata ttctcagatc ggcccgggct
     7021 cgagcagccg tcgctggtag aagagatcca gagatactac ctgaacacgt tgcgaattta
     7081 catcatcaac cagaacagcg cgtcgtcgcg ctgcgccgtg atctacggca ggatcctgag
     7141 cgtgctgacc gagctacgca cgctcggcac gcaaaactcc aacatgtgca tctcgctgaa
     7201 gctgaagaac aggaagctgc cgccgttcct cgaggagatc tgggacgtgg cggaggtggc
     7261 cacgacgcat cccacggtgc tgccgcccac caacccggtg gtgctatagt ctagagggcc
     7321 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
     7381 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     7441 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     7501 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     7561 gcaggcatgc cggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     7621 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7681 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7741 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc
     7801 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7861 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7921 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7981 tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc
     8041 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     8101 aaagtcccca ggctcctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8161 agccag
//
