LOCUS       pEMS2236        4538 bp    DNA     circular      8-JUL-2016
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11316606|
COMMENT     VNTDBDATE|-11316606|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2236|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
COMMENT     VNTOAUTHORNAME|GenScript USA Inc|
COMMENT     VNTOAUTHORTEL|732-885-9188|
COMMENT     VNTOAUTHORFAX|732-210-0262|
COMMENT     VNTOAUTHOREML|gene@genscript.com|
COMMENT     VNTOAUTHORWWW|www.genscript.com|
COMMENT     VNTOAUTHORAD1|GenScript USA Inc|
COMMENT     VNTOAUTHORAD2|120 Centennial Ave|
COMMENT     VNTOAUTHORAD3|Piscataway, NJ 08854, USA|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEMS2236" 0 4538 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT         "NotI" "SalI" "SmaI" "EcoRV" "HindIII" "ScaI" "XbaI" "PstI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 4538 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 2 449 0 3552 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 2270 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 2029 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 397 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 1 437 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "MluI" "acgcgt" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2191 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 2277 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1521 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2300 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4032 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 426 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 540 0 2286 0 0 "") 
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COMMENT        #17=(CRSite (CStringList) "cat" "cat" 1 0 57 38 0 154 0 183 0 230 0 240 
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COMMENT             4446 0 4483 0 4492 0 4497 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "gtg" "gtg" 1 0 79 13 0 22 0 118 0 128 0 177 0 
COMMENT             191 0 193 0 282 0 325 0 394 0 449 0 498 0 609 0 683 0 711 0 821 0 
COMMENT             855 0 873 0 888 0 921 0 965 0 1048 0 1135 0 1140 0 1163 0 1183 0 
COMMENT             1248 0 1251 0 1285 0 1290 0 1389 0 1450 0 1460 0 1493 0 1506 0 1511 
COMMENT             0 1562 0 1576 0 1650 0 1697 0 1796 0 1820 0 1855 0 2083 0 2143 0 
COMMENT             2266 0 2333 0 2335 0 2389 0 2403 0 2414 0 2476 0 2662 0 2776 0 2834 
COMMENT             0 2900 0 2942 0 2970 0 2972 0 3111 0 3130 0 3133 0 3255 0 3346 0 
COMMENT             3495 0 3557 0 3600 0 3687 0 3803 0 3806 0 3886 0 3947 0 4019 0 4026 
COMMENT             0 4057 0 4133 0 4218 0 4267 0 4460 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "cac" "cac" 1 0 59 36 0 59 0 180 0 204 0 370 0 
COMMENT             488 0 490 0 818 0 904 0 1053 0 1186 0 1255 0 1366 0 1413 0 1423 0 
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COMMENT             0 3403 0 3501 0 3624 0 3630 0 3810 0 3954 0 3972 0 4113 0 4213 0 
COMMENT             4221 0 4252 0 4317 0 4444 0 4464 0 4522 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "taa" "taa" 1 0 53 72 0 143 0 214 0 340 0 349 
COMMENT             0 380 0 799 0 833 0 900 0 1044 0 1244 0 1260 0 1329 0 1811 0 2180 0 
COMMENT             2309 0 2385 0 2392 0 2408 0 2420 0 2430 0 2489 0 2616 0 2644 0 2695 
COMMENT             0 2798 0 3016 0 3042 0 3079 0 3139 0 3365 0 3417 0 3422 0 3436 0 
COMMENT             3446 0 3461 0 3489 0 3564 0 3654 0 3724 0 3747 0 3763 0 3934 0 3979 
COMMENT             0 4005 0 4102 0 4128 0 4208 0 4308 0 4423 0 4475 0 4500 0 4507 0 0 
COMMENT             "") 
COMMENT        #21=(CRSite (CStringList) "tta" "tta" 1 0 44 142 0 300 0 339 0 802 0 
COMMENT             1043 0 1216 0 1722 0 2344 0 2429 0 2488 0 2624 0 2862 0 3008 0 3053 
COMMENT             0 3087 0 3195 0 3282 0 3364 0 3386 0 3416 0 3421 0 3435 0 3478 0 
COMMENT             3488 0 3584 0 3644 0 3701 0 3723 0 3762 0 3867 0 3900 0 3950 0 3963 
COMMENT             0 3987 0 4127 0 4181 0 4245 0 4357 0 4369 0 4378 0 4414 0 4485 0 
COMMENT             4488 0 4499 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "tga" "tga" 1 0 58 14 0 17 0 23 0 33 0 172 0 
COMMENT             194 0 395 0 692 0 826 0 868 0 1003 0 1020 0 1033 0 1073 0 1136 0 
COMMENT             1141 0 1148 0 1203 0 1220 0 1322 0 1390 0 1461 0 1471 0 1672 0 2144 
COMMENT             0 2336 0 2411 0 2415 0 2492 0 2557 0 2663 0 2740 0 3028 0 3126 0 
COMMENT             3186 0 3224 0 3318 0 3337 0 3381 0 3428 0 3457 0 3472 0 3496 0 3545 
COMMENT             0 3609 0 3872 0 4020 0 4027 0 4049 0 4191 0 4229 0 4268 0 4328 0 
COMMENT             4361 0 4389 0 4405 0 4468 0 4494 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "tca" "tca" 1 0 81 58 0 101 0 111 0 156 0 232 
COMMENT             0 250 0 369 0 487 0 528 0 649 0 674 0 903 0 971 0 976 0 1040 0 1052 
COMMENT             0 1095 0 1128 0 1191 0 1227 0 1282 0 1382 0 1394 0 1546 0 1694 0 
COMMENT             1703 0 1756 0 2001 0 2149 0 2209 0 2312 0 2318 0 2352 0 2424 0 2444 
COMMENT             0 2553 0 2597 0 2602 0 2606 0 2636 0 2749 0 2765 0 2770 0 2911 0 
COMMENT             2918 0 2934 0 2989 0 3304 0 3343 0 3359 0 3378 0 3389 0 3402 0 3440 
COMMENT             0 3492 0 3509 0 3530 0 3629 0 3647 0 3809 0 3833 0 3837 0 3857 0 
COMMENT             3927 0 3953 0 3957 0 3993 0 4034 0 4043 0 4091 0 4137 0 4140 0 4172 
COMMENT             0 4235 0 4251 0 4335 0 4351 0 4372 0 4386 0 4491 0 4521 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "tag" "tag" 1 0 26 427 0 886 0 1399 0 1887 0 
COMMENT             2321 0 2725 0 2914 0 2926 0 2945 0 3088 0 3105 0 3154 0 3217 0 3250 
COMMENT             0 3407 0 3537 0 3560 0 3742 0 3751 0 3766 0 3901 0 4055 0 4117 0 
COMMENT             4415 0 4431 0 4513 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "cta" "cta" 1 0 29 146 0 297 0 426 0 798 0 899 
COMMENT             0 1243 0 1726 0 1810 0 2177 0 2407 0 2419 0 2795 0 3019 0 3114 0 
COMMENT             3138 0 3142 0 3148 0 3153 0 3327 0 3406 0 3445 0 3504 0 3521 0 3567 
COMMENT             0 3720 0 3741 0 3792 0 4474 0 4504 0 0 "")) 
COMMENT       (CObList 
COMMENT        #26=(CFSignal (CObList) "bla(ApR)" 4 0 1 3478 4338 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3478..4338)") 
COMMENT        #27=(CFSignal (CObList) "rep(pMB1)" 33 0 1 2704 3318 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2704..3318)") 
COMMENT        #28=(CFSignal (CObList) "Ple321 TUBB3" 21 0 0 431 2275 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "431..2275")) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA Plasmid 'pEMS2236'" 1) 
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COMMENT                          "Currently local object. Original author: GenScript USA Inc" 
COMMENT                          1) 
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COMMENT                          "Created: 07/08/16 05:21PM" 1) 
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COMMENT                          "Last Modified: 07/08/16 05:21PM" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4538 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 7-JUL-2016" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "TEL: 732-885-9188  FAX: 732-210-0262" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:gene@genscript.com\">gene@genscript.com</a>" 
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COMMENT                          "WWW: <a href=\"http://www.genscript.com\">www.genscript.com</a>" 
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COMMENT                                        "   complement(3478..4338)" 1))) 
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COMMENT                     #58=(CFolderPar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #62=(CLinePar 
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COMMENT                                        "   431..2275" 1))) "Ple321 TUBB3"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #63=(CFolderPar 
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COMMENT                                        "   complement(2704..3318)" 1))) 
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COMMENT                                        " N1: 449 " 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 3552 " 1))))) "AhdI: 2 sites") 6 5 
COMMENT                          "GACNNNNNGTC" "CTGNNNNNCAG") 
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COMMENT                          "GGCCGGCC" "CCGGCCGG") 
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COMMENT                           3 "AGTACT" "TCATGA") 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Pst}I (2286)" "@N (@S)" 5 -3.32084 
COMMENT                                   -12.1963 0 -10 4.24599 0.932452 #215#)) 
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COMMENT               #217=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 0 100) 
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FEATURES             Location/Qualifiers
     CDS             complement(3478..4338)
                     /vntifkey="4"
                     /label=bla(ApR)
     rep_origin      complement(2704..3318)
                     /vntifkey="33"
                     /label=rep(pMB1)
     misc_feature    431..2275
                     /vntifkey="21"
                     /label=Ple321\TUBB3
BASE COUNT      977 a      1251 c      1336 g       974 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa 
      421 tgcatctaga ggccggccag gggacgtcgt gtcccctggt cctgggaggc tgtccctggc 
      481 ccaaggtcac acagcaggtg cgagagagct gggctggccc ttggttctca tcccctgcag 
      541 gagccaacct ggggctcggg cggggcgggt tgggggcgct gcttgccaaa ggatctgccc 
      601 tccgaacagt gttggggaac aggggatcgg ggggggaggc tggctttgtc aggaccaagg 
      661 ccagggaccc agctcagggc tggtgcagcg ctgattctgg gaaggagggg gtgcaggaga 
      721 cggtcctgga ggggaagggg ccagagaaag gcagcctccg gaggctgttg ccatggaaac 
      781 caggcaggaa caaaaagcta attacaacag ggccagccac gtggctgaca tgtaaattgc 
      841 aactttggct ccgggtggag gcgcctctga gggtgggagg cccagtagtg gggagactct 
      901 aatcacccaa ggagacagct gtgcctgcca aggagagcct gggggagggg agacctgggg 
      961 gacagtggcc tcaaatcaaa ccaggactcc cggagcagcc tctgacagct gccttggcct 
     1021 gatgggaggg gctgagggct cattaatgtg gtcactgggg ccaagcctcc cctgagcatc 
     1081 tgctcctggg agggtcaggg gtcgctgggg ggagggttcg cagctgctca ttttgtgaag 
     1141 tgaatgctga tttgggggcc gggtggggtt cgtctgtaca tcgtgcactg tcagaccctt 
     1201 cctgaaggat ttttgttact gaagtatcag aaggcccttg ttctaaggtg gtggcactgt 
     1261 aatatctggc ccaggaggag gtcagtgccg tgccggcgag tcctttgctc ccttccttct 
     1321 ctgaccagta aacgcatctc cagtcgctcc cccgaggccc ccctgcacgc agtctccgac 
     1381 ctcagagagt gagtcagata gaagccgctg cccaccctcc cccaccctgg ccgggttctt 
     1441 gcctggcttg tgcgtccttg tgacaattcc tgacacggat gcgcaccggg cagtggcacg 
     1501 tccaggtgct gtgcgcgccc gggcccacaa agctcctttg gacgctcatg gcagcccctt 
     1561 ggtggaggag gtcgggtgcc caccccctcc ccgcccactg cggaagccgg cgacccacgg 
     1621 agctcgctct cggccgccgc cacccctctg tgttcgcgcc cttccgagct ctgatccgac 
     1681 gctttgtttc ttctcagtgg gttcagggcc tgggccagcc tttacctacc tcccccaccc 
     1741 aaaaccggca aaagctcaga gcaccttgtc tgccaaaaga cagggagctg ggatggtgcg 
     1801 ggttggtctc taaaccggcg tggggaaaaa agaccctccg tacaaagccg cagggtgggg 
     1861 ctgtcgcaag ggcggaaccg agagggtagc tgggggcggg gttcccaggg ccaagagggg 
     1921 ccattgtcct ccctggagcc cggcgccccc acagccagct cctctgggag acagcccctc 
     1981 ctttcgaatg cgcggggccc tcagaccgcg cccggcccag cgctggggga tccttggctg 
     2041 cgggaggggc gccgcattgc gcgcggcggg cggggacgcg cggtgcggag cctgcgggcc 
     2101 gggcggggct ctgcggcggc gcctcccgat tggccacccg cggtgacatc agccgatgcg 
     2161 aagggcgggg ccgcggctat aagagcgcgc ggccgcggtc cccgaccctc agcagccagc 
     2221 ccggcccgcc cgcgcccgtc cgcagccgcc cgccagacgc gcccagtggc gcgccgtcga 
     2281 ctgcagaggc ctgcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 
     2341 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 
     2401 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 
     2461 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 
     2521 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 
     2581 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 
     2641 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
     2701 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     2761 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     2821 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     2881 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 
     2941 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     3001 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     3061 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     3121 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 
     3181 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     3241 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     3301 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
     3361 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 
     3421 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 
     3481 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 
     3541 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 
     3601 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 
     3661 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 
     3721 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 
     3781 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 
     3841 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 
     3901 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 
     3961 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 
     4021 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 
     4081 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 
     4141 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 
     4201 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 
     4261 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 
     4321 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 
     4381 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 
     4441 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 
     4501 aacctataaa aataggcgta tcacgaggcc ctttcgtc 
//
