LOCUS       pEMS2176        5438 bp    DNA     circular     22-NOV-2016
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11513515|
COMMENT     VNTDBDATE|-11513515|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2176|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
COMMENT     VNTOAUTHORNAME|GenScript USA Inc|
COMMENT     VNTOAUTHORTEL|732-885-9188|
COMMENT     VNTOAUTHORFAX|732-210-0262|
COMMENT     VNTOAUTHOREML|gene@genscript.com|
COMMENT     VNTOAUTHORWWW|www.genscript.com|
COMMENT     VNTOAUTHORAD1|GenScript USA Inc|
COMMENT     VNTOAUTHORAD2|120 Centennial Ave|
COMMENT     VNTOAUTHORAD3|Piscataway, NJ 08854, USA|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT         "XmnI" "PvuI") (CStringList "atg" "gtg") 
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COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5438 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 3133 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 2892 0 3143 0 3164 0 0 
COMMENT            "") #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 397 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 1 460 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "MluI" "acgcgt" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 2 562 0 3054 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 3177 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 8 581 0 676 0 714 0 1063 0 
COMMENT             1154 0 2384 0 3149 0 3170 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 3200 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4932 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 426 0 448 0 0 "") 
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COMMENT        #18=(CRSite (CStringList) "gtg" "gtg" 1 0 81 13 0 22 0 118 0 128 0 177 0 
COMMENT             191 0 193 0 282 0 325 0 394 0 698 0 1101 0 1313 0 1362 0 1473 0 
COMMENT             1547 0 1575 0 1685 0 1719 0 1737 0 1752 0 1785 0 1829 0 1912 0 1998 
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COMMENT             0 1233 0 1243 0 1352 0 1354 0 1682 0 1768 0 1917 0 2049 0 2118 0 
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COMMENT             0 4051 0 4141 0 4260 0 4303 0 4401 0 4524 0 4530 0 4710 0 4854 0 
COMMENT             4872 0 5013 0 5113 0 5121 0 5152 0 5217 0 5344 0 5364 0 5422 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "taa" "taa" 1 0 54 72 0 143 0 214 0 340 0 349 
COMMENT             0 380 0 505 0 1663 0 1697 0 1764 0 1908 0 2107 0 2123 0 2192 0 2674 
COMMENT             0 3043 0 3209 0 3285 0 3292 0 3308 0 3320 0 3330 0 3389 0 3516 0 
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COMMENT             4834 0 4879 0 4905 0 5002 0 5028 0 5108 0 5208 0 5323 0 5375 0 5400 
COMMENT             0 5407 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "tta" "tta" 1 0 48 142 0 300 0 339 0 431 0 
COMMENT             1125 0 1261 0 1271 0 1666 0 1907 0 2079 0 2585 0 3244 0 3329 0 3388 
COMMENT             0 3524 0 3762 0 3908 0 3953 0 3987 0 4095 0 4182 0 4264 0 4286 0 
COMMENT             4316 0 4321 0 4335 0 4378 0 4388 0 4484 0 4544 0 4601 0 4623 0 4662 
COMMENT             0 4767 0 4800 0 4850 0 4863 0 4887 0 5027 0 5081 0 5145 0 5257 0 
COMMENT             5269 0 5278 0 5314 0 5385 0 5388 0 5399 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "tga" "tga" 1 0 62 14 0 17 0 23 0 33 0 172 0 
COMMENT             194 0 395 0 532 0 661 0 871 0 1128 0 1556 0 1690 0 1732 0 1867 0 
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COMMENT             5091 0 5129 0 5168 0 5228 0 5261 0 5289 0 5305 0 5368 0 5394 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "tca" "tca" 1 0 88 58 0 101 0 111 0 156 0 232 
COMMENT             0 250 0 369 0 574 0 825 0 954 0 995 0 1042 0 1183 0 1226 0 1351 0 
COMMENT             1392 0 1513 0 1538 0 1767 0 1835 0 1840 0 1904 0 1916 0 1959 0 1991 
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COMMENT             0 5235 0 5251 0 5272 0 5286 0 5391 0 5421 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "tag" "tag" 1 0 29 427 0 449 0 1207 0 1272 0 
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COMMENT             0 4054 0 4117 0 4150 0 4307 0 4437 0 4460 0 4642 0 4651 0 4666 0 
COMMENT             4801 0 4955 0 5017 0 5315 0 5331 0 5413 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "cta" "cta" 1 0 31 146 0 297 0 426 0 448 0 604 
COMMENT             0 1662 0 1763 0 2106 0 2589 0 2673 0 3040 0 3307 0 3319 0 3695 0 
COMMENT             3919 0 4014 0 4038 0 4042 0 4048 0 4053 0 4227 0 4306 0 4345 0 4404 
COMMENT             0 4421 0 4467 0 4620 0 4641 0 4692 0 5374 0 5404 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 1 5051 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 280 0 4822 0 0 "")) 
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COMMENT        #28=(CFSignal (CObList) "bla(ApR)" 4 0 1 4378 5238 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4378..5238)") 
COMMENT        #29=(CFSignal (CObList) "rep(pMB1)" 33 0 1 3604 4218 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3604..4218)") 
COMMENT        #30=(CFSignal (CObList) "Ple343" 21 0 0 454 3138 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "454..3138")) (CObList) (CObList) (CObList) 
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COMMENT                          "Currently local object. Original author: GenScript USA Inc" 
COMMENT                          1) 
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COMMENT                          "Created: 11/22/16 10:10AM" 1) 
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COMMENT                          "Last Modified: 11/22/16 10:10AM" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                                        "   complement(3604..4218)" 1))) 
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COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 1195459399 
COMMENT       (CStringList) 1414809428 1096042324 
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COMMENT              3.76077e-316 4.57873e-310) 
COMMENT        #284=(CSampFrag "pEMS2176" 1689 3133 "AscI" 4822 "PvuI" (LOGPEN 0 0 0) 
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COMMENT        #285=(CSampFrag "pEMS2176" 896 4822 "PvuI" 280 "PvuI" (LOGPEN 0 0 0) 
COMMENT              1.6322e+069 9.79946e-313) 
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FEATURES             Location/Qualifiers
     CDS             complement(4378..5238)
                     /vntifkey="4"
                     /label=bla(ApR)
     rep_origin      complement(3604..4218)
                     /vntifkey="33"
                     /label=rep(pMB1)
     misc_feature    454..3138
                     /vntifkey="21"
                     /label=Ple343
BASE COUNT     1094 a      1568 c      1647 g      1129 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa 
      421 tgcatctaga ttacctcgcg aatgcatcta gatggccggc ccctgcgctg ggagccgagc 
      481 ccccggcagc tcgcggggat gcagtaagcc gggcgcagcc cgccgggagg atgagaaaac 
      541 ccgaagcccg gaaagccgag cggccgcctg cagtcacccc ggggggggca ggggcgggcc 
      601 gggctatgca gaaacaccgg ggcccgcggg acacaggacg ctgccccact gccgcagagc 
      661 tgaatctgga cccccgggct ttgctttcct ggggcctgtg ctcctccctc cccggggcgg 
      721 ggttcgcctg cagcggcgga ggggggagcc tttgtcctcc gcgcgggctg cggcaccgcc 
      781 tcctcgcggc tgcggggcgg ggctgggggc ggggcctggc cgtctcagcc aatgggaggc 
      841 ggagtccctg gctcgccccg ccctctcgcc tgaaagacct gcggagccgc gggccgggcg 
      901 ggaaccgcat tcgggtcctg gaggggcttg gaggccccag tctcgcagcc ccttcatcgg 
      961 ggacccccgc tcccacctgc cttggccact gccctcaaag cccggccccg ctttccccag 
     1021 gaccccaagc gctgcgcgag gtcagcacca aggacagcgc ccgggccgag cgcctggcct 
     1081 cgaacgccgg gttctgcttt gtgtttccag gtttttgttt ttggttatga gaggaggcct 
     1141 ggctctgtcg cccgggctgg cgtccagagg cgcgatctcg gctcactgca gcctcggcct 
     1201 cccgagtagc tgggaccgca ggcgctcacc accacgcctg gccacaattt tccttttttc 
     1261 ttattttctt ttagatggag tttggctctt gttgcccagg ctgggggacg tcgtgtcccc 
     1321 tggtcctggg aggctgtccc tggcccaagg tcacacagca ggtgcgagag agctgggctg 
     1381 gcccttggtt ctcatcccct gcaggagcca acctggggct cgggcggggc gggttggggg 
     1441 cgctgcttgc caaaggatct gccctccgaa cagtgttggg gaacagggga tcgggggggg 
     1501 aggctggctt tgtcaggacc aaggccaggg acccagctca gggctggtgc agcgctgatt 
     1561 ctgggaagga gggggtgcag gagacggtcc tggaggggaa ggggccagag aaaggcagcc 
     1621 tccggaggct gttgccatgg aaaccaggca ggaacaaaaa gctaattaca acagggccag 
     1681 ccacgtggct gacatgtaaa ttgcaacttt ggctccgggt ggaggcgcct ctgagggtgg 
     1741 gaggcccagt agtggggaga ctctaatcac ccaaggagac agctgtgcct gccaaggaga 
     1801 gcctggggga ggggagacct gggggacagt ggcctcaaat caaaccagga ctcccggagc 
     1861 agcctctgac agctgccttg gcctgatggg aggggctgag ggctcattaa tgtggtcact 
     1921 ggggccaagc ctcccctgag catctgctcc tgggagggtc aggggtcgct ggggggaggg 
     1981 tcgcagctgc tcattttgtg aagtgaatgc tgatttgggg gccgggtggg gttcgtctgt 
     2041 acatcgtgca ctgtcagacc cttcctgaag gatttttgtt actgaagtat cagaaggccc 
     2101 ttgttctaag gtggtggcac tgtaatatct ggcccaggag gaggtcagtg ccgtgccggc 
     2161 gagtcctttg ctcccttcct tctctgacca gtaaacgcat ctccagtcgc tcccccgagg 
     2221 cccccctgca cgcagtctcc gacctcagag agtgagtcag atagaagccg ctgcccaccc 
     2281 tcccccaccc tggccgggtt cttgcctggc ttgtgcgtcc ttgtgacaat tcctgacacg 
     2341 gatgcgcacc gggcagtggc acgtccaggt gctgtgcgcg cccgggccca caaagctcct 
     2401 ttggacgctc atggcagccc cttggtggag gaggtcgggt gcccaccccc tccccgccca 
     2461 ctgcggaagc cggcgaccca cggagctcgc tctcggccgc cgccacccct ctgtgttcgc 
     2521 gcccttccga gctctgatcc gacgctttgt ttcttctcag tgggttcagg gcctgggcca 
     2581 gcctttacct acctccccca cccaaaaccg gcaaaagctc agagcacctt gtctgccaaa 
     2641 agacagggag ctgggatggt gcgggttggt ctctaaaccg gcgtggggaa aaaagaccct 
     2701 ccgtacaaag ccgcagggtg gggctgtcgc aagggcggaa ccgagagggt agctgggggc 
     2761 ggggttccca gggccaagag gggccattgt cctccctgga gcccggcgcc cccacagcca 
     2821 gctcctctgg gagacagccc ctcctttcga atgcgcgggg ccctcagacc gcgcccggcc 
     2881 cagcgctggg ggatccttgg ctgcgggagg ggcgccgcat tgcgcgcggc gggcggggac 
     2941 gcgcggtgcg gagcctgcgg gccgggcggg gctctgcggc ggcgcctccc gattggccac 
     3001 ccgcggtgac atcagccgat gcgaagggcg gggccgcggc tataagagcg cgcggccgcg 
     3061 gtccccgacc ctcagcagcc agcccggccc gcccgcgccc gtccgcagcc gcccgccaga 
     3121 cgcgcccagt ggcgcgccat cggatcccgg gcccgtcgaa tcggatcccg ggcccgtcga 
     3181 ctgcagaggc ctgcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 
     3241 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 
     3301 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 
     3361 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 
     3421 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 
     3481 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 
     3541 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
     3601 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     3661 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     3721 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     3781 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 
     3841 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     3901 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     3961 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     4021 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 
     4081 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     4141 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     4201 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
     4261 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 
     4321 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 
     4381 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 
     4441 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 
     4501 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 
     4561 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 
     4621 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 
     4681 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 
     4741 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 
     4801 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 
     4861 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 
     4921 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 
     4981 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 
     5041 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 
     5101 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 
     5161 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 
     5221 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 
     5281 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 
     5341 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 
     5401 aacctataaa aataggcgta tcacgaggcc ctttcgtc 
//
