LOCUS       pEMS2276        5407 bp    DNA     circular      1-DEC-2016
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11526501|
COMMENT     VNTDBDATE|-11526501|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2276|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
COMMENT     VNTOAUTHORNAME|GenScript USA Inc|
COMMENT     VNTOAUTHORTEL|732-885-9188|
COMMENT     VNTOAUTHORFAX|732-210-0262|
COMMENT     VNTOAUTHOREML|gene@genscript.com|
COMMENT     VNTOAUTHORWWW|www.genscript.com|
COMMENT     VNTOAUTHORAD1|GenScript USA Inc|
COMMENT     VNTOAUTHORAD2|120 Centennial Ave|
COMMENT     VNTOAUTHORAD3|Piscataway, NJ 08854, USA|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEMS2276" 0 5407 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT         "NotI" "SalI" "SmaI" "EcoRV" "HindIII" "ScaI" "XbaI" "PstI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5407 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 3123 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 1149 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 3133 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 397 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 1 408 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "MluI" "acgcgt" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 3146 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 1503 0 2133 0 3049 0 
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COMMENT        #12=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 782 0 3169 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4901 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2372 0 0 "") 
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COMMENT             0 0 "") 
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COMMENT             0 5268 0 5315 0 5352 0 5361 0 5366 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "gtg" "gtg" 1 0 81 13 0 22 0 118 0 128 0 177 0 
COMMENT             191 0 193 0 282 0 325 0 394 0 451 0 463 0 498 0 519 0 572 0 842 0 
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COMMENT             0 4493 0 4499 0 4679 0 4823 0 4841 0 4982 0 5082 0 5090 0 5121 0 
COMMENT             5186 0 5313 0 5333 0 5391 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "taa" "taa" 1 0 81 72 0 143 0 214 0 340 0 349 
COMMENT             0 380 0 481 0 485 0 566 0 671 0 682 0 686 0 692 0 798 0 809 0 855 0 
COMMENT             910 0 980 0 1000 0 1046 0 1064 0 1070 0 1095 0 1101 0 1306 0 1325 0 
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COMMENT             0 3911 0 3948 0 4008 0 4234 0 4286 0 4291 0 4305 0 4315 0 4330 0 
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COMMENT             0 4997 0 5077 0 5177 0 5292 0 5344 0 5369 0 5376 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "tta" "tta" 1 0 71 142 0 300 0 339 0 484 0 507 
COMMENT             0 538 0 632 0 681 0 909 0 962 0 1008 0 1039 0 1045 0 1063 0 1069 0 
COMMENT             1180 0 1211 0 1227 0 1291 0 1324 0 1496 0 1657 0 1684 0 1687 0 1728 
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COMMENT             0 5114 0 5226 0 5238 0 5247 0 5283 0 5354 0 5357 0 5368 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "tga" "tga" 1 0 85 14 0 17 0 23 0 33 0 172 0 
COMMENT             194 0 395 0 430 0 437 0 452 0 464 0 470 0 520 0 527 0 544 0 558 0 
COMMENT             573 0 581 0 609 0 709 0 787 0 835 0 849 0 870 0 883 0 893 0 923 0 
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COMMENT             5363 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "tca" "tca" 1 0 85 58 0 101 0 111 0 156 0 232 
COMMENT             0 250 0 369 0 524 0 548 0 594 0 715 0 772 0 795 0 829 0 931 0 1108 
COMMENT             0 1144 0 1204 0 1208 0 1217 0 1387 0 1445 0 1651 0 1672 0 1738 0 
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COMMENT             3618 0 3634 0 3639 0 3780 0 3787 0 3803 0 3858 0 4173 0 4212 0 4228 
COMMENT             0 4247 0 4258 0 4271 0 4309 0 4361 0 4378 0 4399 0 4498 0 4516 0 
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COMMENT        #24=(CRSite (CStringList) "tag" "tag" 1 0 43 461 0 598 0 628 0 633 0 
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COMMENT             5382 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "cta" "cta" 1 0 52 146 0 297 0 578 0 597 0 627 
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COMMENT       (CObList 
COMMENT        #26=(CFSignal (CObList) "bla(ApR)" 4 0 1 4347 5207 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4347..5207)") 
COMMENT        #27=(CFSignal (CObList) "rep(pMB1)" 33 0 1 3573 4187 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3573..4187)") 
COMMENT        #28=(CFSignal (CObList) "Ple346" 21 0 0 402 3128 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "402..3128")) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA Plasmid 'pEMS2276'" 1) 
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COMMENT                          "Currently local object. Original author: GenScript USA Inc" 
COMMENT                          1) 
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COMMENT                          "Created: 12/01/16 10:36AM" 1) 
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COMMENT                          "Last Modified: 12/01/16 10:36AM" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5407 bp" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 28-NOV-2016" 1))) 
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COMMENT                          "TEL: 732-885-9188  FAX: 732-210-0262" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:gene@genscript.com\">gene@genscript.com</a>" 
COMMENT                          1) 
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COMMENT                          "WWW: <a href=\"http://www.genscript.com\">www.genscript.com</a>" 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #62=(CLinePar 
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COMMENT                                        "   402..3128" 1))) "Ple346"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                        "   complement(3573..4187)" 1))) 
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FEATURES             Location/Qualifiers
     CDS             complement(4347..5207)
                     /vntifkey="4"
                     /label=bla(ApR)
     rep_origin      complement(3573..4187)
                     /vntifkey="33"
                     /label=rep(pMB1)
     misc_feature    402..3128
                     /vntifkey="21"
                     /label=Ple346
BASE COUNT     1350 a      1348 c      1460 g      1249 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cggccggccc ttggtatgtt 
      421 cttttggact gaaaactgat ggagttggaa gtgacttttg tagtgaaaat gaggctggag 
      481 taattaatat tgttccagtg ttctggttat gcgcaggagt gaatcatgat acccaaatta 
      541 caatgactca tctcttctga gagaataaat tgtgattcta tgaaagtttt gcttcactag 
      601 gaattttttg aagggttctg tttgttctag tttagaaacc tacacaggca atatgggctt 
      661 tgcaaaggta taaaattttc ttaaataaaa ctaaacaaag atctattttg attttcagcg 
      721 ttggattttt tttttttctt gcctttcctt tgcttctggg agaaagtatc ttcagacagg 
      781 aagctttgag ctactcataa attctcccta acactgcaga aacatatttc actatgaaat 
      841 ggtggagatg acagtaagag cctcttgttt gaggggcaaa gctgagacat cctgaatgct 
      901 atggaagttt aatcttccat gctgacgcaa tcacgggaat attgtgctcc tggggttttc 
      961 tttattgcca gctactgtct aatgccagga caaaaggaat aatttgatta cagccgagga 
     1021 aagcacagtt tgttcctgtt acttttaaga tgtgcacgca tattaaggtt aacaagattt 
     1081 gtggttgtga agtataattc taaagcctca caagcatgaa gggtgtgtgg acgttttcct 
     1141 gagtcatcct agggaacaaa agggagtggg cagagctagt taccagagtt ggaaagggat 
     1201 gcatcagtca ttacattcag gggctgttag ttggttcctt ttgcaaatgg gctgtgaact 
     1261 gcagatgtcc ctgggagaga aaatagaaaa ttactagcaa attcctaaga gaacctggcc 
     1321 tctttaaaga agagaagaca cattggggct ggggatgaca tcccaaaact cccagcgccg 
     1381 gggagctcag gctcgggcgg tgcctggctc ccctccccgg ttcccgccgc aggaggctgc 
     1441 tgagtcactc ccgcctgagc cacggccgcc cccagccagc cacgcgattg gcgggttagc 
     1501 ccgggcgcag cgccctcttc ccagctcccg cacccccagg gcttcccgca ggcagagggt 
     1561 gggagccccg gcgagcgacc agttggggct tggtaggaac cccctaaaaa tgcgccccac 
     1621 ttgtgcattc gtgcatgggc gcattttggt tcagaattaa ttgagagttg ttcactctac 
     1681 tatttattaa ctagatgatc cgatagttgc tgcaaagtaa cttgtggtta atgcaggtca 
     1741 gtagagagct gatctcactg caggccggag gcaacgccca attcccacgt cttcccctcc 
     1801 cccacccaac aagggctggg cagctttaat gcggaacgcc ggtgcagcag cacggagcac 
     1861 actcccagac tacagtggcg cccgcactca cgcccttcaa gtgccccacc cctcccacac 
     1921 agtggccaag gagccaagcc ctatccctct gcgatctgtc cattgtgcca accctgcatt 
     1981 gcagggaagt cttagcctgt gattccagct tcgctcctgc aaagatagtc actacaggca 
     2041 ggtttaaatt gtatttactc ggagagaaga gggggaaggg aaccccctgc cccctgccaa 
     2101 cctgacaggc caggctgggt gacaagcccc ccgggtggct gcacctccca ctcgccgacc 
     2161 tgggcgctgt ccttggtgct gccggcgcca tagcgagcac aagcgcctta ccttctgccc 
     2221 ctgcggaagc tgcggacgtc ccccaaacgt taaaaacgcc atatcccact ttttatacaa 
     2281 ataactaccc cggcctgttc tctttgtccc cttcccgaag ccttcaggca gaccgttccg 
     2341 caaagtcttt ttttttgttt tgttttgtct tctagaccca gctcctcggc gctcatctcc 
     2401 agctgcttta agacaagggg tgggggaagg ggagggaggc aagaaaagat gagggtgggg 
     2461 gaggggaaaa gagggaatgc aaggggaagg agggaggaga cggggagaag gaaagattgg 
     2521 aagaaaagga tctccgagga aggggctgag agaagggcag ggtgaactgg actaaaggcc 
     2581 agagtaggaa ggagaagagg ggccaaaaaa gaaggggatg aaattaagca cagaagatgg 
     2641 gtaaagaaaa aagtatcagg gaaagggcaa aataagagaa agccttgagg ataagagggt 
     2701 agaaggctaa agaacaaggg gaccactggg tcggggaagc gctgcctgaa cggcgggaca 
     2761 gtgacaaaga aagggcgctg gcgatattcg caccaagggt gcgaaacgca atcgggaggt 
     2821 gagaaatgga aagaaggcga atgcccggct acaagtagcc tgggactgaa aggggacctg 
     2881 ggggaggggc tgggcccagg gcagaaaagt ccaggttccc atgcggcctg ggcccacgtg 
     2941 gagcgggcgc tgaatcaccg ttcagccgcc cccctcccct cctccccgac cggtgcccgc 
     3001 agtccccgcc tcctcggccg ccgcctccac ggggcggggc cctggcccgg gaccagcgcc 
     3061 gcggctataa atgggctgcg gcgaggccgg cagaacgctg tgacagccac acgccccaag 
     3121 ggcgcgccat cggatcccgg gcccgtcgac tgcagaggcc tgcatgcaag cttggcgtaa 
     3181 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 
     3241 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 
     3301 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 
     3361 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 
     3421 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
     3481 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 
     3541 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
     3601 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
     3661 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
     3721 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
     3781 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
     3841 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
     3901 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
     3961 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 
     4021 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
     4081 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 
     4141 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 
     4201 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 
     4261 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 
     4321 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 
     4381 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 
     4441 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 
     4501 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 
     4561 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 
     4621 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 
     4681 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 
     4741 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 
     4801 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 
     4861 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 
     4921 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 
     4981 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 
     5041 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 
     5101 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 
     5161 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 
     5221 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 
     5281 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 
     5341 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 
     5401 tttcgtc 
//
