LOCUS       Exported                7279 bp ds-DNA    circular SYN 20-NOV-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    ppyCAG_RNASEH1_WKKD
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 14, 2018 from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     Ligation of : pCAG DNA Fragment #2 [6416 nt] : (Uncut 5'[0] / Uncut 
            3'[6416]) to human NLS-RNaseH1-V5 XhoI-Not1 [855 nt] : (Uncut 5'[0] 
            / Uncut 3'[855])
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1305..1532
                     /note="bGH PA"
     primer_bind     complement(1575..1595)
                     /note="M13-rev"
     misc_binding    complement(1601..1623)
                     /note="LacO"
     promoter        complement(1628..1657)
                     /note="lac"
     misc_feature    1798..1875
                     /note="SV40 ORI"
     rep_origin      complement(2308..2936)
                     /direction=LEFT
                     /note="ColE1 origin"
     CDS             complement(3088..3747)
                     /note="AmpR"
     misc_feature    5072..6313
                     /note="CAG pro"
                     6421..7275
                     /note="note=""Fragment extracted from human NLS-RNaseH1-V5 
                     XhoI-Not1 [855 nt] : (Uncut 5'[0] / Uncut 3'[855])"
     misc_feature    6428..6451
                     /note="NLS"
     misc_feature    6452..7228
                     /note="RNaseH1"
     misc_feature    7229..7270
                     /note="V5 Tag"
ORIGIN
        1 gctcgataag cttgatatcg aattccgccc cccccccctc tccctccccc ccccctaacg
       61 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
      121 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
      181 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
      241 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
      301 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
      361 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
      421 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
      481 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
      541 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
      601 gatgataata tggccacaac catgaccgag tacaagccca cggtgcgcct cgccacccgc
      661 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
      721 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
      781 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
      841 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
      901 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
      961 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     1021 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     1081 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     1141 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     1201 acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc
     1261 gcacgacccc atgcatcgat gatctagagc tcgctgatca gcctcgactg tgccttctag
     1321 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     1381 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     1441 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     1501 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa cctgcagccc
     1561 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1621 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1681 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1741 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     1801 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     1861 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     1921 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
     1981 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2041 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
     2101 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2161 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2221 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2281 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2341 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2401 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2461 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2521 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2581 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2641 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2701 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2761 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2821 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2881 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2941 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3001 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3061 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3121 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3181 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3241 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3301 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3361 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3421 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3481 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3541 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3601 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3661 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3721 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3781 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3841 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3901 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3961 atgaccgctt tggaaaaaca aagactgtat ttcctggaaa ttaatgttta ttcaataaac
     4021 tgtgtattca gctatattca catagtggtg aggctgaaat gaggcgggaa gaggcggttg
     4081 gggcttaatt atatcaattt gggtggcccc acagcgcctc caaggcgcca gtcctgtttt
     4141 gacaagttgc ctctggaagc ctctcttctt tttctccaga gtaagcggag gccaggggcc
     4201 cccggcctct gcttaatact aaaaaaaaca gctgttgtca tagtaatgat tgggtggaaa
     4261 cattccaggc ctgggtggag aggctttttg cttcctcttg caaaaccaca ctgacattcc
     4321 aggcctgggt ggagaggctt tttgcttcct cttgcaaaac cacactgccc tctggagggc
     4381 agttgcctag caactaatta aaagaggatg tcgcacggcc agctgcggtc agttagtcac
     4441 ttcctgctta actgacttga cattttctat tttaagagtc gggaggaaaa ttactgtgtt
     4501 ggaggccctc cgccatcttc tgaagctgaa tcgaattaac ttgtttattg cagcttataa
     4561 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4621 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatatca
     4681 tcgtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     4741 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     4801 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     4861 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4921 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4981 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     5041 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     5101 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     5161 agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
     5221 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     5281 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     5341 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     5401 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     5461 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     5521 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     5581 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     5641 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     5701 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     5761 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     5821 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     5881 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     5941 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     6001 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     6061 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     6121 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     6181 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     6241 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     6301 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     6361 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcctcga
     6421 gaccatgccc aagaagaaga ggaaggtgtc cttctatgcc gtgaggaggg gccgcaagac
     6481 cggggtcttt ctgaccgcga atgagtgcag agcacaggtg gaccggtttc ctgctgccag
     6541 atttgcggcg tttgccacag aggatgaggc ctgggccttt gtcaggaaat ctgcaagccc
     6601 ggaagtttca gaagggcatg aaaatcaaca tggacaagaa tcggaggcga aagccagcaa
     6661 gcgactccgt gagccactgg atggagatgg acatgaaagc gcagagccgt atgcaaagca
     6721 catgaagccg agcgtggagc cggcgcctcc agttagcaga gacacgtttt cctacatggg
     6781 agacttcgtc gtcgtctaca ctgatggctg ctgctccagt aatgggcgta gaaggccgcg
     6841 agcaggaatc ggcgtttact gggggccggg ccatccttta aatgtaggca ttagacttcc
     6901 tgggcggcag acaaaccaaa gagcggaaat tcatgcagcc tgcaaagcca ttgaacaagc
     6961 aaagactcaa aacatcaata aactggttct gtatacaaac agtatgttta cgataaatgg
     7021 tataactaac tgggttcaag gttggaagaa aaatgggtgg aagacaagtg cagggaaaga
     7081 ggtgatcaac aaagaggact ttgtggcact ggagaggctt acccagggga tggacattca
     7141 gtggatgcat gttcctggtc attcgggatt tataggcaat gaagaagcta acagattagc
     7201 cagagaagga gctaaacaat cggaagacgg taagcctatc cctaaccctc tcctcggtct
     7261 cgattctacg tgagcggcc
//
