LOCUS pAAV-hSynapsin1- 5989 bp DNA linear 25-APR-2018 FEATURES Location/Qualifiers misc_feature 948..954 /note="gcamp3.X" misc_feature 449..920 /note="synapsin promoter" misc_feature 443..448 /note="BglII" misc_feature 2513..2533 /note="Mao 115r" misc_feature 2377..2965 /note="WPRE" misc_feature 2999..3230 /note="SV40" misc_feature 3259..3425 /note="ITR" misc_feature 260..426 /note="ITR" misc_feature 1186..1461 /note="cpGFP 147-238" misc_feature 1462..1479 /note="Linker" misc_feature 1480..1920 /note="cpGFP 1-146" misc_feature 1123..1185 /note="RS20" misc_feature 1015..1122 /note="PRSET Leader" misc_feature 1021..1038 /note="6xHisTag" misc_feature 1921..2361 /note="CaM 2-148" misc_feature 1960..1962 /note="E in G6f" misc_feature 958..1014 /note="GAP43 Sequence" misc_feature 1252..1254 /note="K78H" source 1..5989 /dnas_title="pAAV-hSynapsin1-axon-GCaMP6s" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta 241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc 301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga 361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta 421 tctacgtagc catgctctag gaAGATCTct gcagagggcc ctgcgtatga gtgcaagtgg 481 gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac cccgacccac 541 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg 601 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt 661 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac 721 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg 781 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc 841 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt 901 cgtgcctgag agcgcagtcg aattcaagct gctagcaagg atccaccCGC CACCatgCTG 961 TGCTGTATGA GAAGAACCAA ACAGGTTGAA AAGAATGATG AGGACCAAAA GATTggttct 1021 catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat 1081 ctgtacgacg atgacgataa ggatctcgcc accatggtcg actcatcacg tcgtaagtgg 1141 aataagacag gtcacgcagt cagagctata ggtcggctga gctcactcga gaacgtctat 1201 atcaaggccg acaagcagaa gaacggcatc aaggcgaact tccacatccg ccacaacatc 1261 gaggacggcg gcgtgcagct cgcctaccac taccagcaga acacccccat cggcgacggc 1321 cccgtgctgc tgcccgacaa ccactacctg agcgtgcagt ccaaactttc gaaagacccc 1381 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 1441 ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagcaa gggcgaggag 1501 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 1561 ttcagcgtgt ccggcgaggg tgagggcgat gccacctacg gcaagctgac cctgaagttc 1621 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac 1681 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 1741 gccatgcccg aaggctacat ccaggagcgc accatcttct tcaaggacga cggcaactac 1801 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 1861 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacctgccg 1921 gaccaactga ctgaagagca gatcgcagaa tttaaagagg ctttctccct atttgacaag 1981 gacggggatg ggacaataac aaccaaggag ctggggacgg tgatgcggtc tctggggcag 2041 aaccccacag aagcagagct gcaggacatg atcaatgaag tagatgccga cggtgacggc 2101 acaatcgact tccctgagtt cctgacaatg atggcaagaa aaatgaaata cagggacacg 2161 gaagaagaaa ttagagaagc gttcggtgtg tttgataagg atggcaatgg ctacatcagt 2221 gcagcagagc ttcgccacgt gatgacaaac cttggagaga agttaacaga tgaagaggtt 2281 gatgaaatga tcagggaagc agacatcgat ggggatggtc aggtaaacta cgaagagttt 2341 gtacaaatga tgacagcgaa gtaaAAGCTT atcgataatc aacctctgga ttacaaaatt 2401 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 2461 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 2521 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 2581 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 2641 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 2701 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 2761 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg 2821 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 2881 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 2941 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgacccgg gcggccgctt 3001 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga 3061 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc 3121 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag 3181 atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat 3241 cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta actacaagga 3301 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg 3361 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc 3421 gcgcagcctt aattaaccta attcactggc cgtcgtttta caacgtcgtg actgggaaaa 3481 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 3541 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 3601 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 3661 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 3721 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 3781 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 3841 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 3901 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 3961 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 4021 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat 4081 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 4141 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 4201 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 4261 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 4321 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 4381 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 4441 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 4501 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 4561 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 4621 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 4681 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 4741 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 4801 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 4861 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 4921 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 4981 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 5041 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 5101 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 5161 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 5221 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 5281 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 5341 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 5401 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 5461 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 5521 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 5581 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 5641 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 5701 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 5761 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 5821 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 5881 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 5941 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag //