LOCUS       pAAV-hSynapsin1-        6730 bp    DNA     circular     25-APR-2018
FEATURES             Location/Qualifiers
     enhancer        2868..3456
                     /note="WPRE"
     misc_feature    3004..3024
                     /note="Mao 115r"
     misc_feature    3490..3721
                     /note="SV40"
     misc_feature    3750..3916
                     /note="ITR"
     misc_feature    27..193
                     /note="ITR"
     misc_feature    4680..5540
                     /note="Ampicillin"
     promoter        4610..4638
                     /note="Ampicillin Promoter"
     misc_feature    5695..6313
                     /note="pBR322_ori"
     misc_feature    4111..4417
                     /note="f1 origin"
     promoter        216..687
                     /note="hSynapsin1_Promoter"
     misc_feature    2071..2136
                     /note="PTV-1 2A"
     misc_feature    714..720
                     /note="gcamp3.X"
     misc_feature    724..831
                     /note="PRSET Leader"
     misc_feature    730..747
                     /note="6xHisTag"
     misc_feature    895..1170
                     /note="cpGFP 147-238"
     misc_feature    1171..1188
                     /note="Linker"
     misc_feature    1189..1629
                     /note="cpGFP 1-146"
     misc_feature    832..894
                     /note="RS20 Peptide"
     misc_feature    1630..2070
                     /note="CaM 2-148"
     misc_feature    1669..1671
                     /note="E in G6f"
     misc_feature    2137..2844
                     /label=mRuby3
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     source          1..6730
                     /dnas_title="pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3"
ORIGIN      
        1 CGCAGAATTT AAAGAGGtaa ttaggctgcg cgctcgctcg ctcactgagg ccgcccgggc
       61 aaagcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag
      121 agagggagtg gccaactcca tcactagggg ttccttgtag ttaatgatta acccgccatg
      181 ctacttatct acgtagccat gctctaggaa gatctctgca gagggccctg cgtatgagtg
      241 caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc
      301 gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg
      361 gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca
      421 gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg
      481 acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc
      541 gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac
      601 catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga
      661 gtcgtgtcgt gcctgagagc gcagtcgaat tcaagctgct agctagtacc ggtCGCCACC
      721 atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
      781 ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt
      841 cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ctcactcgag
      901 aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt ccacatccgc
      961 cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc
     1021 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg
     1081 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1141 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag
     1201 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1261 ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc
     1321 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1381 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1441 ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac
     1501 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1561 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1621 aacctgccgg accaactgac tgaagagcag atcgcagaat ttaaagaggc tttctcccta
     1681 tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct
     1741 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac
     1801 ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa aatgaaatac
     1861 agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc
     1921 tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat
     1981 gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac
     2041 gaagagtttg tacaaatgat gacagcgaag ggatccggag ccacgaactt ctctctgtta
     2101 aagcaagcag gagacgtgga agaaaacccc ggtcctGTGT CTAAGGGCGA AGAGCTGATC
     2161 AAGGAAAATA TGCGTATGAA GGTGGTCATG GAAGGTTCGG TCAACGGCCA CCAATTCAAA
     2221 TGCACAGGTG AAGGAGAAGG CAGACCGTAC GAGGGAGTGC AAACCATGAG GATCAAAGTC
     2281 ATCGAGGGAG GACCCCTGCC ATTTGCCTTT GACATTCTTG CCACGTCGTT CATGTATGGC
     2341 AGCCGTACCT TTATCAAGTA CCCGGCCGAC ATCCCTGATT TCTTTAAACA GTCCTTTCCT
     2401 GAGGGTTTTA CTTGGGAAAG AGTTACGAGA TACGAAGATG GTGGAGTCGT CACCGTCACG
     2461 CAGGACACCA GCCTTGAGGA TGGCGAGCTC GTCTACAACG TCAAGGTCAG AGGGGTAAAC
     2521 TTTCCCTCCA ATGGTCCCGT GATGCAGAAG AAGACCAAGG GTTGGGAGCC TAATACAGAG
     2581 ATGATGTATC CAGCAGATGG TGGTCTGAGA GGATACACTG ACATCGCACT GAAAGTTGAT
     2641 GGTGGTGGCC ATCTGCACTG CAACTTCGTG ACAACTTACA GGTCAAAAAA GACCGTCGGG
     2701 AACATCAAGA TGCCCGGTGT CCATGCCGTT GATCACCGCC TGGAAAGGAT CGAGGAGAGT
     2761 GACAATGAAA CCTACGTAGT GCAAAGAGAA GTGGCAGTTG CCAAATACAG CAACCTTGGT
     2821 GGTGGCATGG ACGAGCTGTA CAAGtaagct tactagagct tatcgataat caacctctgg
     2881 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2941 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3001 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3061 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3121 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3181 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3241 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     3301 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3361 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3421 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgacccg
     3481 ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     3541 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     3601 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     3661 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     3721 tcgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt
     3781 aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     3841 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     3901 agcgagcgag cgcgcagcct taattaacct aattcactgg ccgtcgtttt acaacgtcgt
     3961 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     4021 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     4081 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4141 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4201 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4261 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4321 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4381 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4441 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4501 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     4561 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4621 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4681 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4741 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4801 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4861 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4921 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4981 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5041 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5101 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5161 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5221 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5281 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5341 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5401 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5461 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5521 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5581 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5821 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6361 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6421 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6481 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6541 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     6601 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6661 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
     6721 attaaggcct
//

