LOCUS       pEC245_pRS306-OsTIR1.xdna    7047 bp      DNA     circular   4/13/18
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    411..1214
                     /label=URA3
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    196..409
                     /label=URA3pr
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1928..2165
                     /label=MCS
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    50..67
                     /label=oEC750 R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="GDGHSL" \ 
     misc_feature    4271..4287
                     /label=oEC749
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="VPRTFX" \ 
     misc_feature    1601..1618
                     /label=partial oEC752 R
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="RGAVKH" \ 
     misc_feature    1296..1314
                     /label=oEC753
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="GVKYRTX" \ 
     misc_feature    4374..5073
                     /label=ADH1pr
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5074..6798
                     /label=OsTIR1
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVGNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEELRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVEDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLR \ 
     misc_feature    5786..5806
                     /label=oEC773rev
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="GI*" \ 
     misc_feature    5656..5674
                     /label=oEC772
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="AGSLERX" \ 
     misc_feature    6845..7047
                     /label=Adh1t
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5590..5590
                     /label=New Feature
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="X" \ 
     misc_feature    6799..6804
                     /label=new_stop(s)
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="*" \ 
     misc_feature    6489..6510
                     /label=oEC946
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="EWMQEFKX" \ 
     misc_feature    1004..1023
                     /label=oEC992
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="DYDTRCX" \ 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 
      301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat 
      361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag 
      421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata 
      481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat 
      541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata 
      601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt 
      661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc 
      721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg 
      781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac 
      841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat 
      901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg 
      961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg 
     1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg 
     1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg 
     1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg 
     1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta 
     1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt 
     1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta 
     1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 
     1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 
     1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 
     1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta 
     1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 
     1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 
     1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg 
     1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 
     1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca 
     1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta 
     1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcccccggg 
     2041 ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc 
     2101 cagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct 
     2161 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat 
     2221 aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc 
     2281 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 
     2341 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     2401 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     2461 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     2521 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctcggcc cccctgacga 
     2581 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     2641 ccaggcgttc ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     2701 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg 
     2761 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     2821 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     2881 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     2941 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 
     3001 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     3061 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
     3121 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     3181 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     3241 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     3301 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     3361 tcgttcatcc atagttgcct gactgcccgt cgtgtagata actacgatac gggagggctt 
     3421 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     3481 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     3541 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     3601 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 
     3661 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     3721 gtgaaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     3781 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     3841 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     3901 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 
     3961 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     4021 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     4081 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     4141 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
     4201 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     4261 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 
     4321 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcGGGTGTA 
     4381 CAATATGGAC TTCCTCTTTT CTGGCAACCA AACCCATACA TCGGGATTCC TATAATACCT 
     4441 TCGTTGGTCT CCCTAACATG TAGGTGGCGG AGGGGAGATA TACAATAGAA CAGATACCAG 
     4501 ACAAGACATA ATGGGCTAAA CAAGACTACA CCAATTACAC TGCCTCATTG ATGGTGGTAC 
     4561 ATAACGAACT AATACTGTAG CCCTAGACTT GATAGCCATC ATCATATCGA AGTTTCACTA 
     4621 CCCTTTTTCC ATTTGCCATC TATTGAAGTA ATAATAGGCG CATGCAACTT CTTTTCTTTT 
     4681 TTTTTCTTTT CTCTCTCCCC CGTTGTTGTC TCACCATATC CGCAATGACA AAAAAATGAT 
     4741 GGAAGACACT AAAGGAAAAA ATTAACGACA AAGACAGCAC CAACAGATGT CGTTGTTCCA 
     4801 GAGCTGATGA GGGGTATCTC GAAGCACACG AAACTTTTTC CTTCCTTCAT TCACGCACAC 
     4861 TACTCTCTAA TGAGCAACGG TATACGGCCT TCCTTCCAGT TACTTGAATT TGAAATAAAA 
     4921 AAAAGTTTGC TGTCTTGCTA TCAAGTATAA ATAGACCTGC AATTATTAAT CTTTTGTTTC 
     4981 CTCGTCATTG TTCTCGTTCC CTTTCTTCCT TGTTTCTTTT TCTGCACAAT ATTTCAAGCT 
     5041 ATACCAAGCA TACAATCAAC TATCTCATAT ACAATGACGT ACTTCCCGGA GGAGGTGGTG 
     5101 GAGCACATCT TCAGCTTCCT GCCGGCGCAG CGCGACCGCA ACACGGTCTC GCTCGTCTGC 
     5161 AAGGTGTGGT ACGAGATCGA GAGGCTGAGC CGCCGCGGCG TCTTCGTGGG CAACTGCTAC 
     5221 GCCGTGCGCG CCGGCCGCGT CGCCGCGCGG TTCCCCAACG TGCGGGCGCT CACGGTGAAG 
     5281 GGGAAGCCCC ACTTCGCCGA CTTCAACCTC GTGCCCCCCG ACTGGGGCGG CTACGCGGGG 
     5341 CCGTGGATCG AGGCGGCCGC GAGGGGATGC CACGGCCTGG AGGAGCTCAG GATGAAGCGG 
     5401 ATGGTGGTGT CCGACGAGAG CCTCGAGCTG CTGGCTCGCT CGTTCCCGCG GTTCAGGGCT 
     5461 CTTGTTCTTA TCAGCTGCGA GGGGTTCAGC ACTGACGGGC TAGCCGCCGT CGCGAGCCAT 
     5521 TGCAAGCTTC TGAGGGAGTT GGATTTGCAG GAAAATGAAG TGGAGGATCG AGGGCCTAGG 
     5581 TGGCTTTCCT GCTTCCCTGA TTCCTGCACA TCACTTGTCT CATTGAATTT TGCCTGCATC 
     5641 AAAGGGGAGG TTAATGCTGG TTCACTGGAG AGACTTGTTA GCAGGTCCCC AAACCTGCGG 
     5701 AGTTTGAGGC TGAATCGATC TGTATCGGTA GATACACTTG CAAAGATACT ACTGCGTACC 
     5761 CCTAACTTGG AGGATTTGGG GACAGGGAAT TTGACAGATG ACTTCCAAAC TGAGTCCTAC 
     5821 TTTAAGCTTA CCAGTGCTCT GGAGAAATGC AAGATGTTGA GGAGTTTGTC TGGATTCTGG 
     5881 GATGCTTCTC CTGTTTGCCT GTCATTTATC TACCCCCTGT GTGCTCAACT GACAGGATTG 
     5941 AACTTGAGCT ATGCCCCCAC ACTTGATGCT TCTGACCTTA CAAAAATGAT TAGCCGCTGT 
     6001 GTGAAGCTCC AACGCCTTTG GGTACTGGAT TGTATCTCGG ACAAAGGCTT GCAAGTGGTG 
     6061 GCCTCCAGTT GCAAAGACTT GCAAGAACTC AGGGTATTTC CATCAGATTT CTACGTAGCT 
     6121 GGTTATTCTG CAGTGACAGA GGAGGGACTT GTTGCAGTAT CCTTGGGCTG TCCAAAACTG 
     6181 AACTCACTAC TGTACTTCTG TCACCAAATG ACTAATGCTG CACTAGTTAC TGTCGCCAAG 
     6241 AACTGTCCAA ATTTCACACG ATTCAGACTT TGTATTCTTG AGCCAGGGAA GCCTGATGTT 
     6301 GTGACAAGCC AACCATTAGA TGAAGGCTTT GGAGCTATTG TTCGTGAGTG CAAGGGATTA 
     6361 CAACGTTTGT CAATATCTGG TCTTCTCACA GACAAAGTTT TCATGTATAT TGGGAAATAT 
     6421 GCAAAACAAC TTGAGATGCT TTCTATAGCA TTTGCTGGTG ACAGTGATAA GGGTATGATG 
     6481 CATGTTATGA ATGGATGCAA GAATTTAAGG AAACTGGAGA TAAGAGATAG CCCGTTTGGT 
     6541 GATGCTGCAC TCTTGGGGAA TTTTGCTAGG TACGAGACAA TGCGATCCCT TTGGATGTCA 
     6601 TCTTGCAATG TCACGTTAAA GGGGTGCCAA GTCCTTGCGT CAAAGATGCC GATGCTCAAT 
     6661 GTTGAGGTCA TAAATGAGCG GGATGGTAGC AATGAAATGG AGGAAAACCA TGGAGATCTG 
     6721 CCTAAAGTGG AGAAATTATA TGTGTACCGC ACAACTGCTG GGGCGAGGGA TGATGCACCA 
     6781 AATTTTGTTA AAATCCTAtG atAgGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 
     6841 GTAAcacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta 
     6901 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct 
     6961 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg 
     7021 ctcttattga ccacacctct accggca
//