LOCUS       pEC254_pRS316_GAL_EGFP    6519 bp      DNA     circular   4/13/18
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    2001..2006
                     /label=SacII
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2029..2034
                     /label=BamHI
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    196..1292
                     /label=URA3 cassette
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(3743..3762)
                     /label=T3
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     1942..1959
                     /label=M13-fwd
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3780..3800)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     1966..1985
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4168..4796)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      1353..1793
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(1343..1798)
                     /label=M13 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(1803..1871)
                     /label=LacZ alpha
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(3806..3828)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(4948..5607)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5847..5875)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(3015..3056)
                     /label=V5 tag
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(2988..3008)
                     /label=TEV site
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      5937..6447
                     /label=CEN-ARS pRS
                     /SerialCloner_Color=&hFF6FCF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3057..3062
                     /label=EcoRI
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(2247..2963)
                     /label=yEGFP
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(3060..3684)
                     /label=GAL1 promoter
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(2047..2226)
                     /label=ADH1t
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2265..2265
                     /label=New Feature
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(2387..2406)
                     /label=oEC723
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="LDHHQLX" \ 
     misc_feature    complement(2270..2290)
                     /label=oEC724
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="HG*" \ 
     misc_feature    2783..2803
                     /label=oEC725
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="LRLAMEL" \ 
     misc_feature    6430..6450
                     /label=oEC979
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="VSYRQAI" \ 
     misc_feature    complement(50..67)
                     /label=oEC750
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="GDGHSL" \ 
     misc_feature    5903..5919
                     /label=oEC749
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="VPRTFX" \ 
     misc_feature    2696..2715
                     /label=oEC726
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="LAWQT*" \ 
     misc_feature    complement(3117..3140)
                     /label=oEC1013
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation" \ 
                     /SerialCloner_Desc=translation="DI*" \ 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 
      241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 
      301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat 
      361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag 
      421 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata 
      481 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat 
      541 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata 
      601 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt 
      661 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc 
      721 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg 
      781 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac 
      841 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat 
      901 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg 
      961 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg 
     1021 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg 
     1081 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg 
     1141 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg 
     1201 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta 
     1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt 
     1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta 
     1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 
     1441 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 
     1501 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 
     1561 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta 
     1621 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 
     1681 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 
     1741 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg 
     1801 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 
     1861 ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg ggtaacgcca 
     1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta 
     1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcccccggg 
     2041 ctgcagggtg tggtcaataa gagcgacctc atactatacc tgagaaagca acctgaccta 
     2101 caggaaagag ttactcaaga ataagaattt tcgttttaaa acctaagagt cactttaaaa 
     2161 tttgtataca cttatttttt ttataactta tttaataata aaaatcataa atcataagaa 
     2221 attcgcttat ttagaagtgg cgcgccttat ttgtacaatt catccatacc atgggtaata 
     2281 ccagcagcag taacaaattc taacaagacc atgtggtctc tcttttcgtt tggatctttg 
     2341 gataaggcag attgagtgga taagtaatgg ttgtctggta acaagactgg accatcacca 
     2401 attggagtat tttgttgata atggtcagct aattgaacag aaccatcttc aatgttgtgt 
     2461 ctaattttga agttaacttt gataccattc ttttgtttgt cagccatgat gtaaacattg 
     2521 tgagagttat agttgtattc caatttgtga cctaaaatgt taccatcttc tttaaaatca 
     2581 atacctttta attcgattct attaactaag gtatcacctt caaacttgac ttcagctctg 
     2641 gtcttgtagt taccgtcatc tttgaaaaaa atagttcttt cttgaacata accttctggc 
     2701 atggcagact tgaaaaagtc atgttgtttc atatgatctg ggtatctaga aaaacattga 
     2761 acaccataag ttaaagtagt gactaaggtt ggccatggaa ctggcaattt accagtagta 
     2821 caaataaatt ttaaggtcaa tttaccgtaa gtagcatcac cttcaccttc accggagaca 
     2881 gaaaatttgt gaccattaac atcaccatct aattcaacca aaattgggac aacaccagtg 
     2941 aataattctt cacctttaga catgttaatt aaaccagcac cgtcaccTCC TTGAAAATAC 
     3001 AAATTTTCtg aaccCGTAGA ATCGAGACCG AGGAGAGGGT TAGGGATAGG CTTACCgaat 
     3061 tcTCCTTGAC GTTAAAGTAT AGAGGTATAT TAACAATTTT TTGTTGATAC TTTTATGACA 
     3121 TTTGAATAAG AAGTAATACA AACTGAAAAT GTTGAAAGTA TTAGTTAAAG TGGTTATGCA 
     3181 GCTTTTCCAT TTATATATCT GTTAATAGAT CAAAAATCAT CGCTTCGCTG ATTAATTACC 
     3241 CCAGAAAcAA GGCTAAAAAA CTAATCGCAT TATCATCCTA TGGTTGTTAA TTTGATTCGT 
     3301 TAATTTGAAG GTTTGTGGGG CCAGGTTACT GCCAATTTTT CCTCTTCATA ACCATAAAAG 
     3361 CTAGTATTGT AGAATCTTTA TTGTTCGGAG CAGTGCGGCG CGAGGCACAT CTGCGTTTCA 
     3421 GGAACGCGAC CGGTGAAGAC GAGGACGCAC GGAGGAGAGT CTTCCGTCGG AGGGCTGTCG 
     3481 CCCGCTCGGC GGCTTCTAAT CCGTACTTCA ATATAGCAAT GAGCAGTTAA GCGTATTACT 
     3541 GAAAGTTCCA AAGAGAAGGT TTTTTTAGGC TAAGATAATG GGGCTCTTTA CATTTCCACA 
     3601 ACATATAAGT AAGATTAGAT ATGGATATGT ATATGGTGGT AATGCCATGT AATATGATTA 
     3661 TTAAACTTCT TTGCGTCCAT CCAAgatatc aagcttatcg ataccgtcga cctcgagggg 
     3721 gggcccggta cccagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc 
     3781 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatagg 
     3841 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac tcacattaat 
     3901 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 
     3961 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 
     4021 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 
     4081 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
     4141 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctcgg 
     4201 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     4261 actataaaga taccaggcgt tcccccctgg aagctccctc gtgcgctctc ctgttccgac 
     4321 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     4381 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     4441 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     4501 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     4561 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     4621 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     4681 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     4741 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     4801 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
     4861 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 
     4921 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 
     4981 gatctgtcta tttcgttcat ccatagttgc ctgactgccc gtcgtgtaga taactacgat 
     5041 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     5101 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     5161 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     5221 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 
     5281 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     5341 atcccccatg ttgtgaaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     5401 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     5461 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     5521 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 
     5581 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     5641 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
     5701 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
     5761 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
     5821 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
     5881 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc 
     5941 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa 
     6001 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg 
     6061 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc 
     6121 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg 
     6181 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt 
     6241 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt 
     6301 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca 
     6361 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac 
     6421 gaggcgcgtg taagttacag gcaagcgatc cgtcctaaga aaccattatt atcatgacat 
     6481 taacctataa aaataggcgt atcacgaggc cctttcgtc
//