LOCUS       peAA.11                 3052 bp    DNA     circular      2-DEC-2011
SOURCE      
  ORGANISM  
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            http://www.invitrogen.com/
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COMMENT                                            " N1: 1049 " 1))))) "BstXI: 1 site") 
COMMENT                            8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
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COMMENT                            4 "ATCGAT" "TAGCTA") 
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COMMENT                            1 5 "GAATTC" "CTTAAG") 
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COMMENT                                            " N2: 854 " 1))))) "FseI: 2 sites") 
COMMENT                            6 2 "GGCCGGCC" "CCGGCCGG") 
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COMMENT                            1 5 "AAGCTT" "TTCGAA") 
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COMMENT                            "CGTAACTATAACGGTCCTAAGGTAGCGAA" 
COMMENT                            "GCATTGATATTGCCAGGATTCCATCGCTT") 
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COMMENT                            1 "GGTACC" "CCATGG") 
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COMMENT                            26 24 "TCCRACNNNNNNNNNNNNNNNNNNNNN" 
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COMMENT                            2 6 "CGCCGGCG" "GCGGCCGC") 
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COMMENT                            5 "CCATGG" "GGTACC") 
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COMMENT                            6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                            1 "ATGCAT" "TACGTA") 
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COMMENT                            5 3 "TTAATTAA" "AATTAATT") 
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COMMENT                            1 6 "TCCNGGA" "AGGNCCT") 
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COMMENT                            "ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG" 
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COMMENT                            4 4 "GTTTAAAC" "CAAATTTG") 
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COMMENT                            2 6 "VCTCGAGB" "BGAGCTCV") 
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COMMENT                            1 "CTGCAG" "GACGTC") 
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COMMENT                            2 "CCTGCAGG" "GGACGTCC") 
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COMMENT                            2 6 "CRCCGGYG" "GYGGCCRC") 
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COMMENT                            5 "ACTAGT" "TGATCA") 
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FEATURES             Location/Qualifiers
     misc_feature    771..806
                     /vntifkey="21"
                     /label=LoxP
                     /note="*vntifkey: 21"
     misc_feature    138..173
                     /vntifkey="21"
                     /label=LoxP
                     /note="*vntifkey: 21"
     misc_feature    329..384
                     /vntifkey="21"
                     /label=telLR
     primer          413..472
                     /vntifkey="27"
                     /label=for_05
     primer          649..650
                     /vntifkey="27"
                     /label=for_09
     primer          complement(623..648)
                     /vntifkey="27"
                     /label=rev_08
     primer          complement(354..442)
                     /vntifkey="27"
                     /label=rev_04
     primer          complement(649..680)
                     /vntifkey="27"
                     /label=rev_09
     CDS             complement(2193..3050)
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    1005..1024
                     /vntifkey="21"
                     /label=symL
     misc_feature    856..875
                     /vntifkey="21"
                     /label=symL
     primer_bind     967..984
                     /vntifkey="28"
                     /label=vec.hrr.for07\annealing
     misc_feature    887..915
                     /vntifkey="21"
                     /label=I-CeuI
     primer_bind     complement(896..921)
                     /vntifkey="28"
                     /label=pA.hrf.rev07\annealing
BASE COUNT      753 a       785 c       745 g       769 t 
ORIGIN
        1 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
       61 tatttgaatg tatttagaaa aataaacaaa taggggaatt aaaaaaaagc ccgctcatta 
      121 ggcgggctac tagtcagata acttcgtata atgtatgcta tacgaagtta tggctcgagg 
      181 aattcctgca gggaacccat aaaatgcaat cgtagtccac ctgatcgtac ttagaaatga 
      241 gggtcccctt ttgcccacgc accagttcgc tcgtcgtttg cttttaagaa ccgcacgaac 
      301 cacagagcat ttaattaaaa cgccggcgta tcagcacaca attgcccatt atacgcgcgt 
      361 ataatggact attgtgtgct gatacattgc cattctccga gtgacgccgg cgagagaacc 
      421 tctagctcct ttacaaagta ctggttccct ttccagcggg atgccttatc taaacgcaat 
      481 gacagacgta ttcctcaggc cacatcgctt cctacttccg ctgggatcca tcggcgcgcc 
      541 attgacggcc gaagccgcca ttccatagtg agtccttcgt ctgtgtcttt ctgtgccaga 
      601 tcgtctagca aattgccgat ccatagtcgt acagaccgaa atggccggcc ttaattaact 
      661 taagtcaaat cacgcgacta ggctcagctc tattttagtg gtcatgggtt ttggtccgcc 
      721 cgagcggtgc acccgattag gaccatgtaa aacatttaag cttcccatgg ataacttcgt 
      781 ataatgtatg ctatacgaag ttatgggggt accgcggccg cgtagaggag atgtaggtgt 
      841 tggcaccggc cggccaattg tgagcgctca caattactcg agtatgcata actataacgg 
      901 tcctaaggta gcgacctagg tatcgattct agaaatatac ttctaatagt acaaggcctc 
      961 tcgagaggct gctgccaccg ctgagcaata actagcggcg cgccaattgt gagcgctcac 
     1021 aattcgccgg cggtttaaac ccatctaatt gggaattccc cctgcctcgc gcgtttcggt 
     1081 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa 
     1141 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg 
     1201 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg 
     1261 catcagagca gattgtactg agagtgcacc attgcggtgt gaaataccgc acagatgcgt 
     1321 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     1381 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     1441 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     1501 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     1561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     1621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     1681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     1741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     1801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     1861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     1921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
     1981 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     2041 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     2101 aaaaaaagga tctcaagaag atcctttgat cttttctacc ggtccgtcca aaaaaaaagg 
     2161 ctccaaaagg agcctttaat tgtatcggtt taccaatgct taatcagtga agcccctatt 
     2221 tcagcaatct gtctatttcg ttcgtccata gttgcctgac tacccgtcgt gtagataact 
     2281 acgatacggg aaggcttacc atctggtcca agtgcggcaa tgataccccg tgaaccacgc 
     2341 tctcctgccc cagatttatc agcaataaac cagccagcag gaagtgccga acgcagtagt 
     2401 ggtcctgcaa ctttatccgc ttcaagccag tctattaatt gttgccgtga agccagagta 
     2461 agtagttcgc cagttaatag tttgcgtaat gttgttgcca ttgccgcagg cattgtggtg 
     2521 tctcgctcgt cgtttggtat ggcttcattc agttctggtt cccaacggtc aaggcgagtt 
     2581 acatggtctc ccatattgtg caaaaaagcg gttaattcct tcggtccacc gatggttgtc 
     2641 agaagtaagt tcgccgcagt gttatcactc atcgttatgg cggcactaca taattctctt 
     2701 accgtcattc cgtccgtaag gtgcttttct gtcactgggg agtattcaac caagtcattc 
     2761 tgcgaatagt gtattcgtcg tccgagttgc tcttgccccg catctacacg ggataatact 
     2821 gctccacata gcagaacttt gaaagtgctc atcattggaa aacgctcttc agggcgaaaa 
     2881 ctctcaagga ttttaccgct attcaagtcc agttctatgt atcccactcg tgcccccaat 
     2941 tggtcctctg cgtcttttac tttcacgagc gtttctgggt gagcaaaaac aggaaggcaa 
     3001 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat ac 
//
