LOCUS       peDK.11                 2573 bp    DNA     circular      2-DEC-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|608480331|
COMMENT     LSOWNER|
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COMMENT                            15 11 "ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG" 
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FEATURES             Location/Qualifiers
     misc_feature    908..943
                     /vntifkey="21"
                     /label=LoxP
                     /note="*vntifkey: 21"
     misc_feature    275..310
                     /vntifkey="21"
                     /label=LoxP
                     /note="*vntifkey: 21"
     misc_feature    466..521
                     /vntifkey="21"
                     /label=telLR
     primer          550..609
                     /vntifkey="27"
                     /label=for_05
     primer          786..787
                     /vntifkey="27"
                     /label=for_09
     primer          complement(760..785)
                     /vntifkey="27"
                     /label=rev_08
     primer          complement(491..579)
                     /vntifkey="27"
                     /label=rev_04
     primer          complement(786..817)
                     /vntifkey="27"
                     /label=rev_09
     CDS             complement(1124..1915)
                     /vntifkey="4"
                     /label=KanR
                     /note="*vntifkey: 4"
     misc_feature    67..86
                     /vntifkey="21"
                     /label=symL
     misc_feature    224..243
                     /vntifkey="21"
                     /label=symL
     primer_bind     186..203
                     /vntifkey="28"
                     /label=vec.hrr.for07\annealing
     misc_feature    95..131
                     /vntifkey="21"
                     /label=PI-SceI
                     /note="*vntifkey: 21"
     primer_bind     119..140
                     /vntifkey="28"
                     /label=annealing\pD.hrf.rev07
     misc_feature    complement(2503..52)
                     /vntifkey="21"
                     /label=ori
     misc_feature    2166..2282
                     /vntifkey="21"
                     /label=ori
BASE COUNT      604 a       706 c       640 g       623 t 
ORIGIN
        1 gagcttagta cgttaaacat gagagcttag tacgtgaaac atgagagctt agcccggggg 
       61 ccggccaatt gtgagcgctc acaattactc gagtatctat gtcgggtgcg gagaaagagg 
      121 taatgaaatg gcacctaggt atcgattcta gaaatatact tctaatagta caaggcctct 
      181 cgagaggctg ctgccaccgc tgagcaataa ctagcggcgc gccaattgtg agcgctcaca 
      241 attcgccggc ggtttaaacc catgtgcctg gcagataact tcgtataatg tatgctatac 
      301 gaagttatgg ctcgaggaat tcctgcaggg aacccataaa atgcaatcgt agtccacctg 
      361 atcgtactta gaaatgaggg tccccttttg cccacgcacc agttcgctcg tcgtttgctt 
      421 ttaagaaccg cacgaaccac agagcattta attaaaacgc cggcgtatca gcacacaatt 
      481 gcccattata cgcgcgtata atggactatt gtgtgctgat acattgccat tctccgagtg 
      541 acgccggcga gagaacctct agctccttta caaagtactg gttccctttc cagcgggatg 
      601 ccttatctaa acgcaatgac agacgtattc ctcaggccac atcgcttcct acttccgctg 
      661 ggatccatcg gcgcgccatt gacggccgaa gccgccattc catagtgagt ccttcgtctg 
      721 tgtctttctg tgccagatcg tctagcaaat tgccgatcca tagtcgtaca gaccgaaatg 
      781 gccggcctta attaacttaa gtcaaatcac gcgactaggc tcagctctat tttagtggtc 
      841 atgggttttg gtccgcccga gcggtgcacc cgattaggac catgtaaaac atttaagctt 
      901 cccatggata acttcgtata atgtatgcta tacgaagtta tgggtacgta gtgggcgaag 
      961 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt 
     1021 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg 
     1081 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa 
     1141 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc 
     1201 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct 
     1261 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt 
     1321 ccaccatgat attcggcaag caggcatcgc catgtgtcac gacgagatcc tcgccgtcgg 
     1381 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt 
     1441 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat 
     1501 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg 
     1561 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc 
     1621 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag 
     1681 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt 
     1741 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca 
     1801 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata 
     1861 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa 
     1921 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg 
     1981 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag 
     2041 ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa 
     2101 gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc 
     2161 agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgttcc 
     2221 gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgaagct aattctgtca 
     2281 gccgttaagt gttcctgtgt cactgaaaat tgctttgaga ggctctaagg gcttctcagt 
     2341 gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt aaaagcctta 
     2401 tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt tgctgattta 
     2461 tattaatttt attgttcaaa catgagagct tagtacgtga aacatgagag cttagtacgt 
     2521 tagccatgag agcttagtac gttagccatg agggtttagt tcgttaaaca tga 
//
